Citation: LIU Chun-Bo, SHEN Qin-Peng, ZHANG Feng-Mei, ZHANG An-Feng, HE Pei, SI Xiao-Xi, LIU Zhi-Hua, YANG Guang-Yu, MIAO Ming-Ming. Determination of Benzo[a]pyrene in Mainstream Cigarette Smoke by Online Comprehensive Two-dimensional Liquid-Gas Chromatography[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(2): 241-246. doi: 10.11895/j.issn.0253-3820.150666 shu

Determination of Benzo[a]pyrene in Mainstream Cigarette Smoke by Online Comprehensive Two-dimensional Liquid-Gas Chromatography

1.
Key Lab of Tobacco Chemistry of Yunnan, Technology Center, China Tobacco Yunnan Industrial Co., Ltd., Kunming 650231, China

Corresponding author: YANG Guang-Yu,
Received Date: 21 August 2015
Available Online: 5 November 2015
Fund Project: 本文系国家自然科学基金(No. 21365026)资助项目 (No. 21365026)

An online comprehensive two-dimensional liquid-gas chromatography method was developed to determine the benzo[a]pyrene (B[a]P) in mainstream cigarette smoke. The B[a]P in mainstream cigarette smoke was collected by a cambridge filter pad, then the pad was extracted with cyclohexane and the extract was quantitatively analyzed by online LC-GC/MS with B[a]P-D12 as an internal standard. After direct injection into the performance liquid chromatography, the benzo[a]pyrene in the samples were separated by micro silica gel column and cut into the gas chromatography. After draining the solvent, the benzo[a]pyrene was separated by capillary column gas chromatography warming and detected by mass spectrometry detection. By using silica gel column chromatography and gas chromatography-mass spectrometry in combination, the benzo[a]pyrene in the mainstream cigarette smoke was directly detected without any sample pretreatment. The sample injection volume of the method could reach 40 μL each time, which was far greater than that of conventional gas chromatography-mass spectrometry (2.0 μL of sample injection volume), resulting in an increasing analysis sensitivity of 20 times. The linearity range of the method (R²=0.999) was in the range of 0.08 ng/L to 50 ng/L. The recoveries at three spiked levels ranged from 94.2% to 105.5% and the limit of detection was 0.09 ng/cig. This method was applied for the determination of benzo[a]pyrene in 14 kind cigarettes in Chinese marked and the reference cigarettes 2R4F, and the determination results were consistent with GB/T21130-2007 method.
- Online comprehensive two-dimensional liquid-gas chromatography,
- Benzo[a]pyrene,
- Cigarette,
- Mainstream smoke
1. [1]
  1 LIU Jian-Fu, YU Xi, LIU De-Hua. Chinese Journal of Chromatography, 2002, 20(2): 187-189 刘建福, 喻昕, 刘德华. 色谱, 2002, 20(2): 187-189
2. [2]
  2 SHIJia-Xin, LIU Bai-Zhan, XIE Wen-Yan. Chinese Journal of Chromatography, 2010, 28(6): 623-627 史佳沁, 刘百战, 谢雯燕. 色谱, 2010, 28(6): 623-627
3. [3]
  3 DU Xue-Qing, WANG Xin-Ming, TANG Jian-Hui. Environmental Chemistry, 2006, 25(6): 785-788 杜雪晴, 王新明, 唐建辉. 环境化学, 2006, 25(6): 785-788
4. [4]
  4 Wenzl T, Simon R, Anklam E, Kleiner J. TrAC-Trend. Anal. Chem., 2006, 25: 716-725
5. [5]
  5 Jung K H, Noh J H, Eun J W, Kim J K, Bae H J, Xie H, Jang J J, Ryu J C, Park W S, Lee J Y. Environmental Science & Technology, 2010, 45: 300-306
6. [6]
  6 FAN Hu, LIU Shao-Min, SHENG Liang-Quan. Tobacco Science Technology, 2004, 9: 36-39 樊虎, 刘少民, 盛良全. 烟草科技, 2004, 9: 36-39
7. [7]
  7 ZHANG Jun-Song, DAI Yong, JIA Chun-Xiao. Tobacco Science Technology, 2003, 4: 22-24 张峻松, 戴勇, 贾春晓. 烟草科技, 2003, 4: 22-24
8. [8]
  8 ZHANG Xia, LIU Zhi-Hua, KONG Wei-Song. Chinese Journal of Analysis Laboratory, 2013, 10: 100-102 张霞, 刘志华, 孔维松. 分析试验室, 2013, 10: 100-102
9. [9]
  9 CHENG Qian, ZHANG Hong-Fei, HOU Hong-Wei. Tobacco Science Technology, 2012, 2: 29-32 程倩, 张洪非, 侯宏卫. 烟草科技, 2012, 2: 29-32
10. [10]
  10 LU Hong. Analytical Instrumentation, 2010, 3: 65-69 鲁红. 分析仪器, 2010, 3: 65-69
11. [11]
  11 ZHAO Zi-Gang, LÜ Jian-Hua, WANG Jian. Science and Technology of Cereals, Oils and Foods, 2010, 18(2): 47-50 赵子刚, 吕建华, 王建. 粮油食品科技, 2010, 18(2): 47-50
12. [12]
  12 Hyötyläinen T, Riekkola M L. Anal. Bioanal. Chem., 2004, 378: 1936-1938
13. [13]
  13 Hyötyläinen T, Riekkola M L. J. Chromatogr. A, 2003, 1000: 357-384
14. [14]
  14 Purcaro G, Moret S, Conte L. J Chromatogr. A, 2012, 1255: 100-111
15. [15]
  15 LUO Yan-Bo, ZHENG Hao-Bo, JIANG Xing-Yi. Chinese J. Anal. Chem., 2015, 43(10): 1538-1544 罗彦波, 郑浩博, 姜兴益. 分析化学, 2015, 43(10): 1538-1544
16. [16]
  16 GB/T 5606.1-2004, Cigarette Part 1: sampling 卷烟第1部分抽样. 中华人民共和国国家标准. GB/T 5606.1-2004
17. [17]
  17 GB/T 19609-2004, Cigarette Total Particle Phase is Measured by Routine Analysis Using Smoking Machine and Tar Content 卷烟用常规分析用吸烟机测定总粒相物和焦油. 中华人民共和国国家标准. GB/T 19609-2004
18. [18]
  18 The Principle and Application of GPC-GCMS System. Island Ferry International Trading (Shanghai) co., LTD GPC-GCMS系统原理及应用. 岛津国际贸易(上海)有限公司
19. [19]
  19 GB/T 21130-2007, Cigarette Total Grain Content in Flue Gas with the Determination of Benzene and [a] Pyrene 卷烟烟气总粒相物中苯并[a]芘的测定. 中华人民共和国国家标准. GB/T 21130-2007
20. [20]
  20 Chen P X, Moldoveanu S C. Beiträge zur Tabakforschung International, 2003, 20: 448-458
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沈阳化工大学材料科学与工程学院沈阳 110142