Citation: WEI Lin-Hong, LIU Lin, LÜ Hong-Ying, TENG Zhen-Yuan, KANG Hui-Min, WANG Cheng-Yin, HU Xiao-Ya. An Impedance Immunosensor for Detection of Uniconazole Based on Screen Printed Electrode[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(2): 258-264. doi: 10.11895/j.issn.0253-3820.150391 shu

An Impedance Immunosensor for Detection of Uniconazole Based on Screen Printed Electrode

1.
¹ College of Chemistry and Chemical Engineering, Jiangsu Key Laboratory of Environmental Engineering and Materials, Yangzhou University, Yangzhou 225002, China;
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² College of Biological and Chemical Engineering, Yangzhou Vocational University, Yangzhou 225009, China

Corresponding author: WANG Cheng-Yin,
Received Date: 12 May 2015
Available Online: 18 November 2015
Fund Project: 本文系国家自然科学基金(No. 21375116)江苏省高等职业院校国内高级访问学者计划资助项目(No.2014FX092)江苏省环境材料与环境工程重点实验室2014年度开放基金项目(No.K14019)资助项目 (No. 21375116)江苏省高等职业院校国内高级访问学者计划资助项目(No.2014FX092)江苏省环境材料与环境工程重点实验室2014年度开放基金项目(No.K14019)

Polyaniline/chitosan composite membrane was modified on the surface of a screen printed carbon electrode by cyclic voltammetry (CV), and then anti-uniconazole antibodies were immobilized on the electrode based on electrostatic attraction. The impedance immunosensor for the determination of uniconazole was thus constructed. CV and electrochemical impedance spectroscopy (EIS) were used to characterize the electrochemical properties of immunosensor. Several factors affecting the immunoreaction were investigated, including the pH of phosphate buffer saline (PBS), incubation temperature and time. The performance of the proposed immunosensor was investigated under the optimized conditions. The immunosensor exhibited advantages of high accuracy, high sensitivity and specificity, good reproducibility and stability. The immunosensor could detect uniconazole in a linear range of 0.01-100 mg/L (R=0.998), and the detection limit was 8 μg/L (3σ). The average recoveries were 98.5%-104.6% and the relative standard deviations were 3.3-4.3% at the spiked level of 0.05, 0.10 and 1.0 mg/kg in cabbage, and the detection results were consistent with the results obtained by gas chromatography.
- Immunosensor,
- Electrochemical impedance spectroscopy,
- Uniconazole,
- Screen printed electrode
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