Citation: LI Jian-Wen, WEI Shou-Lian, Jiang Jie-Tao. Preparation and Application of Semicarbazide Electrochemical Sensor Based on Molecularly Imprinting Technique[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(1): 103-109. doi: 10.11895/j.issn.0253-3820.150180 shu

Preparation and Application of Semicarbazide Electrochemical Sensor Based on Molecularly Imprinting Technique

1.
¹ Guangdong Food and Drug Vocational College, Guangzhou 510620, China;
2.
² Faculty of Chemistry and Chemical Engineering, Zhaoqing University, Zhaoqing 526061, China

Corresponding author: WEI Shou-Lian,
Received Date: 8 April 2015
Available Online: 6 November 2015
Fund Project: 本文系广东省教育厅科技创新项目(No.2012kjcx0104) (No.2012kjcx0104)肇庆市科技计划项目(No.2013C019)资助 (No.2013C019)

A molecularly imprinted electrochemical sensor was prepared by electropolymerization using semicarbazide (SEM) as template molecule and o-phenylenediamine as functional monomer in 0.1 mol/L NaAc-HAc buffer solution (pH 5.2). The performance and surface feature of the proposed imprinted sensor was investigated using cyclic voltammetry (CV), differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS) and scanning electron microscope. The sensor had a rough and porous surface morphology, and showed good recognition and selectivity to SEM. The sensor performance was optimized by using DPV method in 0.5 mol/L KCl solution with 5 mmol/L K₃[Fe(CN)₆] as the electron transport medium. The optimized conditions were that the molar ratio of functional monomer to template molecule was 1:2, the elution liquid was 0.2 mol/L NaOH, and elution and incubation time was 15 and 12 min, respectively. The DPV peak current difference of the sensor had a linear relationship with the concentration of SEM in the range of 3.75-188 ng/L and the detection limit was 1.5 ng/L (S/N=3). The prepared imprinted sensor was used to detect SEM in commercially available fresh shrimp muscles as well as shrimp contaminated with drugs with satisfactory results, and the recoveries of SEM were in the range of 80%-97%.
- o-Phenylenediamine,
- Electropolymerization,
- Molecular imprinted electrode,
- Shrimp,
- Semicarbazide
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

Preparation and Application of Semicarbazide Electrochemical Sensor Based on Molecularly Imprinting Technique

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

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通讯作者: 陈斌, bchen63@163.com