Citation: Jian-xu Zhang, Ting-ting Sun, Zhi-gang Xie. Dye-protein Hybrid Nanoparticles for Photothermal Therapy of Tumor Cells[J]. Acta Polymerica Sinica, ;2019, 50(6): 633-641. doi: 10.11777/j.issn1000-3304.2019.19001 shu

Dye-protein Hybrid Nanoparticles for Photothermal Therapy of Tumor Cells

State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022

Corresponding author: Ting-ting Sun, suntt@ciac.ac.cn Zhi-gang Xie, xiez@ciac.ac.cn
Received Date: 3 January 2019
Revised Date: 12 February 2019
Available Online: 28 March 2019

To make up for the inherent drawbacks (e.g. hydrophobicity, toxicity, and instability) of near-infrared Cypate dyes, as photothermal agents, stable hybrid nanoparticles (CBNPs) with small size were prepared from bovine serum albumin (BSA) and Cypate dyes under hydrothermal conditions. Free Cypate exhibited strong fluorescence whereas fluorescence quenching in CBNPs caused a sharp decrease in emission peak intensity, so the dye molecules were successfully assembled into protein nanoparticles. As-prepared CBNPs were characterized with dynamic light scattering (DLS) and transmission electron microscopy (TEM). The hydrodynamic diameter of CBNPs was around 25 – 40 nm with a low polydispersity index of 0.2, slightly higher than the measured values from TEM observation. BSA encapsulation could significantly improve the water solubility of Cypate dye and the stability of dye aqueous solutions. Besides, these nanoparticles showed good colloidal stability and biocompatibility. Photothermal experiments suggested that the protein nanoparticles had good photothermal performance and were able to generate enough heat under near-infrared laser irradiation. The photothermal conversion efficiency (η) of CBNPs under 808 nm laser irradiation reached up to 50%, which made them outperform the small molecules of neat Cypate dyes in terms of their photothermal conversion capabilities. Confocal laser scanning microscopy further revealed that the protein nanoparticles could be efficiently internalized by cancer cells in a time-dependent manner, which is very important for their therapeutic functions. Photothermal treatment toward human cervical carcinoma (HeLa) cells and human liver hepatocellular carcinoma (HepG2) cells under laser irradiation was extamined by MTT method, in which the protein nanoparticles showed an effective inhibition effect on tumor cell proliferation at the cellular level. Live/dead cell staining experiments conducted on HeLa cells also showed the same results intuitively. The dye-protein hybrid nanoparticles developed in this study as a novel nano-agent possess promising prospects in the field of tumor photothermal therapy.
- Protein nanoparticles,
- Cypate dyes,
- Supramolecular assembly,
- Photothermal therapy
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