Citation: Ruo-yu Cheng, Wen-guo Cui. Hydrogen Bond Mechanically Enhanced Cholecalciferol Lipo-Hydrogel for Bone Regeneration[J]. Acta Polymerica Sinica, ;2018, 0(10): 1315-1327. doi: 10.11777/j.issn1000-3304.2018.18066 shu

Hydrogen Bond Mechanically Enhanced Cholecalciferol Lipo-Hydrogel for Bone Regeneration

Ruo-yu Cheng ¹ ,
Wen-guo Cui ^1,2,3,,

1.
Orthopedic Institute, Medicine Department, Soochow University, Suzhou 215006
2.
Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025
3.
State Key Laboratory of Molecular Engineering of polymer, Department of Macromolecular Science, Fudan Univeristy, Shanghai 200433

Corresponding author: Wen-guo Cui, wgcui80@hotmail.com
Received Date: 26 February 2018
Revised Date: 27 March 2018
Available Online: 6 September 2018

Hydrogel is an excellent candidate for drug delivery and tissue regeneration. However, hydrogel used as drug carriers is facing some problems, including limited optional drug and burst drug release. In addition, hydrogel is still confronting some challenges in the area of mechanical property. Cholecalciferol is a type of vitamin able to promote ossification. However, as a lipophilic drug, it should be taken according to the recommended dosage. That means the carriers must be capable of making hydrophobic drug homodispersed with controlling drug release. In this study, we designed a novel hydrogen bond based mechanical reinforced hydrogel, modified by release-controlling cholecalciferol liposome, as an multifunctional scaffold for promoting osteogenesis during the process of bone regeneration. Specifically, drug-loaded liposome was fabricated and the characteristics of the liposomes were investigated. Further, photo-crosslinkable gelatin derivative (GelMA) was combined with drug loaded liposome by micro-cross linking double network, generated by the interaction between hydrogen bond and hydrogel network, leading to double enhancement in mechanical property both for compression and streching. Furthermore, the ability to control release and support uniform distribution of hydrophobic drug (cholecalciferol) was observed in the composite hydrogel, specifically the sustained release of cholecalciferol lasted for three weeks at least without significant brust release. In vitro study also revealed that, this composite hydrogel was proved to have excellent biocompatibility without significant influence on MC3T3-E1 cell adhesion, proliferation, compared with GelMA hydrogel and control groups. After 21 days cultivation, MC3T3-E1 cells cultured with this composite hydrogel exhibited remarkably facilitated osteogenic differentiation, according to the result of ALP stain, ALP analysis and Alizarin red staining. Thus, it is reasonable to believe that this liposome composite hydrogel could provide a promising strategy for extending the application of hydrogel in drug delivery and tissue reconstruction.
- Liposome,
- Hydrogel,
- Control release,
- Mechanical enhancement,
- Bone regeneration
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