Citation: Ying Wang, Zhengna Duan, Jin Shen, Ruilong Song, Xiaoya Hu, Yun Shu. Metal-organic framework mediated magnetic nanozyme enhanced lateral flow immunoassay diagnosis[J]. Chinese Chemical Letters, ;2026, 37(7): 112230. doi: 10.1016/j.cclet.2025.112230 shu

Metal-organic framework mediated magnetic nanozyme enhanced lateral flow immunoassay diagnosis

Ying Wang ^a ,
Zhengna Duan ^a ,
Jin Shen ^a ,
Ruilong Song ^b ,
Xiaoya Hu ^a ,
Yun Shu ^{a, *,}

a.
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China
b.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, School of Veterinary Medicine, Yangzhou University, Yangzhou 225002, China

shuyun@yzu.edu.cn

Received Date: 14 July 2025
Revised Date: 10 October 2025
Accepted Date: 7 December 2025
Available Online: 8 December 2025

Figures(5)

Iron oxide based magnetic catalytic colorimetric (MCC) nanomaterials have attracted extensive attention as signal labels in lateral flow immunoassays (LFIA). However, the main challenge in this field is its weak catalytic activity leading to relatively low color intensity. Herein, a new strategy of using the metal-organic framework (MOF) as mediate interfacing layer for loading Pt nanoparticles (Pt NPs) on the magnetic nanoparticles (MNPs) was proposed to obtain MCC nanomaterials with high catalytic activity. First, Cu-Co MOF was grown in situ with MNPs as the core, and then the MOF interfacing layer facilitates the uniform growth of Pt NPs on it. The obtained MNPs@MOF@Pt nanocomposite exhibited enhanced color signal intensity, excellent peroxidase-like activity and strong magnetic separation ability. After integration with a dual-antibody sandwich LFIA platform, the highly sensitive detection of interleukin-6 was achieved with a detection limit of 8.3 and 2.8 pg/mL for the pre-catalytic and post-catalytic tests respectively, which were 58-fold and 171-fold higher than that of the LFIA based on gold nanoparticles. Moreover, it exhibited good specificity and high accuracy for detection of clinical serum samples. Therefore, the magnetic nanozyme material proposed in this study demonstrated great potential for application in point-of-care diseases diagnosis.
- Lateral flow immunoassay,
- Multifunctional magnetic nanozyme,
- Colorimetric catalytic signal,
- Point-of-care testing
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沈阳化工大学材料科学与工程学院沈阳 110142