Citation: Yue Cao, Yue Lin, Li Chen, Huimin Niu, Renli Wei, Shuqian Qiu, Anjie Wang, Xiaoai Cao, Xiaofeng Lai, Yongshou Chen, Juanjuan Lin, Shuiliang Wang, Zhenyu Lin, Shenghang Zhang. Screening of glial fibrillary acidic protein specific aptamer and application in the development of fluorescent biosensor based on isothermal amplification strategy[J]. Chinese Chemical Letters, ;2026, 37(5): 111616. doi: 10.1016/j.cclet.2025.111616 shu

Screening of glial fibrillary acidic protein specific aptamer and application in the development of fluorescent biosensor based on isothermal amplification strategy

Yue Cao ^{a, 1} ,
Yue Lin ^{c, 1} ,
Li Chen ^{a, b, 1} ,
Huimin Niu ^{a, b} ,
Renli Wei ^b ,
Shuqian Qiu ^b ,
Anjie Wang ^a ,
Xiaoai Cao ^a ,
Xiaofeng Lai ^{a, b} ,
Yongshou Chen ^{a, b} ,
Juanjuan Lin ^{a, b} ,
Shuiliang Wang ^{a, d, *,} ,
Zhenyu Lin ^{c, *,} ,
Shenghang Zhang ^{a, b, *,}

a.
Department of Clinical Laboratory Medicine, Fuzong Clinical Medical College of Fujian Medical University, Fuzhou 350025, China
b.
Fujian Key Laboratory of Aptamers Technology, Fuzong Teaching Hospital of Fujian University of Traditional Chinese Medicine (900th Hospital), Fuzhou 350025, China
c.
Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, China
d.
Department of Clinical Laboratory Medicine, Dongfang Hospital of School of Medicine, Xiamen University, Fuzhou 350025, China

shuiliang.wang@xmu.edu.cn

zylin@fzu.edu.cn

fzzyyzsh@126.com

Received Date: 29 April 2025
Revised Date: 25 June 2025
Accepted Date: 19 July 2025
Available Online: 19 July 2025

Figures(4)

Glial fibrillary acidic protein (GFAP) can serve as a promising early blood biomarker for Alzheimer's disease (AD). Existing assays mostly rely on antibody-based detection technologies, the preparation of antibodies is relatively complex, costly, and requires high storage conditions. In this study, we screened an aptamer specifically targeting GFAP (K_D = 0.621 µmol/L) through systematic evolution of ligands by exponential enrichment (SELEX) technique for the first time and then applied which to develop a simple but sensitive fluorescent sensor by combining isothermal exponential amplification reaction (EXPAR) with hybridization chain reaction (HCR). The platform achieved a broad linear detection range (10 pg/mL to 10 µg/mL) and a low detection limit (0.24 pg/mL). The results detected by the proposed sensor were highly correlated with that detected by ELISA method (R = 0.9989, P < 0.0001). The work overcomes the limitations of antibody-based technologies and provides a promising solution for early diagnosis of AD.
- Glial fibrillary acidic protein,
- Aptamer,
- SELEX,
- Fluorescence,
- Alzheimer's disease
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