Citation: Xiaoyan Liu, Cong Xu, Ruhe Zhang, Yilu Zheng, Hengyu Liu, Haolin Chen, Meng Zhao, Jun Wu, Dongjun Lin. Black phosphorus nanosheets-based platform for B-cell lymphoma chemo-photothermal therapy[J]. Chinese Chemical Letters, ;2026, 37(5): 111401. doi: 10.1016/j.cclet.2025.111401 shu

Black phosphorus nanosheets-based platform for B-cell lymphoma chemo-photothermal therapy

Xiaoyan Liu ^{a, b, 1} ,
Cong Xu ^{a, 1} ,
Ruhe Zhang ^{a, 1} ,
Yilu Zheng ^{a, 1} ,
Hengyu Liu ^a ,
Haolin Chen ^{c, *,} ,
Meng Zhao ^{a, d, *,} ,
Jun Wu ^{a, e, f, *,} ,
Dongjun Lin ^{a, *,}

a.
Department of Hematology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, China
b.
Department of Hematology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China
c.
Department of Anesthesiology, General Hospital of Southern Theater Command of PLA, Guangzhou 510010, China
d.
Key Laboratory of Stem Cells and Tissue Engineering (Ministry of Education), Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China
e.
Bioscience and Biomedical Engineering Thrust, The Hong Kong University of Science and Technology (Guangzhou), Guangzhou 511400, China
f.
Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong 999077, China

chenhlin8@mail2.sysu.edu.cn

zhaom38@mail.sysu.edu.cn

junwuhkust@ust.hk

lindj@mail.sysu.edu.cn

Received Date: 24 March 2025
Revised Date: 27 May 2025
Accepted Date: 29 May 2025
Available Online: 30 May 2025

Figures(5)

In the treatment of B-cell lymphoma, chemotherapy as a monotherapy encounters significant challenges like drug resistance, side effects, and limited cytotoxicity. A novel strategy combining chemotherapy and photothermal therapy uses nanomaterials to convert light into heat, locally heating tumor tissues to induce thermal ablation while enhancing the effectiveness of chemotherapeutic agents and reducing toxic side effects on normal cells. Here, we developed a multifunctional black phosphorus nanosheets (BP NSs) for chemo-photothermal synergistic therapy of lymphoma. BP NSs were synthesized from bulk black phosphorus crystal powders utilizing a modified liquid exfoliation technique and functionalized with polyethylene glycol (PEG) to improve stability. The PEGylated BP NSs were loaded with two chemotherapeutic agents, gemcitabine (Gem) and doxorubicin (DOX), forming GD-BP@PEG NSs. The nanosheets exhibit excellent physical stability, efficient photothermal conversion, and pH/near-infrared (NIR) dual-responsive drug release. In vitro cell experiments demonstrated that GD-BP@PEG NSs significantly increased cytotoxicity and apoptosis, especially with NIR laser irradiation. Furthermore, in vivo studies in A20 lymphoma-bearing BALB/c nude mice revealed GD-BP@PEG NSs passively accumulated with high concentrations at the tumor site, efficiently inhibiting lymphoma growth with minimal systemic toxicity, demonstrating significant advantages over single treatments of chemotherapy or photothermal therapy alone. In summary, this pH/NIR dual-triggered BP NSs system could serve as a promising nanoplatform for chemo-photothermal synergistic treatment of B-cell lymphoma.
- Black phosphorus nanosheets,
- pH/NIR dual-triggered,
- B-cell lymphoma,
- Chemotherapy,
- Photothermal therapy
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