Citation: Yi Liu, Xiaolin Yu, Wenyun Mu, Minsi Meng, Baixue Li, Jie Liu, Haixin Qian, Lin Weng, Tingting Yu, Nan Hu, Xin Chen, Yi Hao. Substrate-independent nano-coating with persistent antibacterial and tooth whitening activities for dental health[J]. Chinese Chemical Letters, ;2026, 37(5): 111338. doi: 10.1016/j.cclet.2025.111338 shu

Substrate-independent nano-coating with persistent antibacterial and tooth whitening activities for dental health

Yi Liu ^{a, b, c, d, 1} ,
Xiaolin Yu ^{b, d, e, 1} ,
Wenyun Mu ^{c, 1} ,
Minsi Meng ^{a, c, 1} ,
Baixue Li ^{a, c} ,
Jie Liu ^c ,
Haixin Qian ^f ,
Lin Weng ^c ,
Tingting Yu ^a ,
Nan Hu ^a ,
Xin Chen ^{c, *,} ,
Yi Hao ^{f, *,}

a.
College of Chemical Engineering, Sichuan University of Science & Engineering, Zigong 643000, China
b.
Institute of Precision Medicine, Zigong Academy of Big Data and Artificial Intelligence in Medical Science, Zigong Fourth People's Hospital, Zigong 643000, China
c.
Department of Chemical Engineering, Shaanxi Key Laboratory of Energy Chemical Process Intensification, Institution of Polymer Science in Chemical Engineering, School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an 710049, China
d.
Sichuan Clinical Research Center for Clinical Laboratory, Zigong Fourth People's Hospital, Zigong 643000, China
e.
Department of Laboratory Medicine, Zigong Fourth People's Hospital, Zigong 643000, China
f.
Department of Prosthodontics, Shanghai Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center of Stomatology, Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, Shanghai 200240, China

chenx2015@xjtu.edu.cn

haoyi5315@163.com

Received Date: 11 March 2025
Revised Date: 15 May 2025
Accepted Date: 16 May 2025
Available Online: 17 May 2025

Figures(5)

Bacteria and stains on tooth and various dental materials severely harm dental health and beauty and require feasible solutions. In this study, a simple strategy was developed to produce nano-coating on different substrates for persistent antibacterial and whitening. The coating is formed by the lysozyme (Lys), hemoglobin (Hb), and glucose oxidase (GOD) via co-assembly, in which the phase transition of Lys initiated the co-assembly to anchor other two proteins. During therapy, the GOD continuously oxidizes glucose in the oral environment to cut off the nutrition of bacteria meanwhile generating H₂O₂, which would be further catalyzed by the ferrous ions in Hb to produce reactive oxygen species (ROS) for effective decomposition of surrounding bacteria and stains. Moreover, the Hb can perform persistent release of oxygen, which not only enhances the efficiency of glucose oxidation to produce more ROS but directly suppresses anaerobic bacteria via reversing the local hypoxia environment in the mouth. The experimental results indicated that our strategy is able to form nano-film of proteins both on the surface of dental orthosis and human tooth, which further causes obvious reduction of the bacteria not only on the coated substrate but in the surrounding tissue with up to 100% of the bacteriostatic rate. In addition, both the dental orthosis and human tooth were also rapidly cleaned due to the local ROS generation, leading to a sustained anti-staining property in the long term.
- Substrate-independent coating,
- Nano-assembly of proteins,
- Manipulation of oral microenvironment,
- Persistent antibacterial,
- Teeth whitening
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