Citation: Jinwen Xiao, Xiaojun Ai, Conglai Zhou, Ruiqi Feng, Junrong Chen, Juan Wu, Xiongbo Song, Long Chen. Fabrication and characteristics of decellularized amniotic membrane-based nanofiber composite for tissue repair[J]. Chinese Chemical Letters, ;2026, 37(5): 111291. doi: 10.1016/j.cclet.2025.111291 shu

Fabrication and characteristics of decellularized amniotic membrane-based nanofiber composite for tissue repair

Jinwen Xiao ^{a, 1} ,
Xiaojun Ai ^{a, 1} ,
Conglai Zhou ^{a, 1} ,
Ruiqi Feng ^a ,
Junrong Chen ^b ,
Juan Wu ^c ,
Xiongbo Song ^b ,
Long Chen ^{b, *,}

a.
Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
b.
Department of Orthopedics, Guizhou Provincial People's Hospital, Guiyang 550000, China
c.
Wuhan Kangchuang Biotechnology, Wuhan 430074, China

longchen@gzu.edu.cn

Received Date: 8 March 2025
Revised Date: 4 May 2025
Accepted Date: 8 May 2025
Available Online: 9 May 2025

Figures(4)

Decellularized amniotic membrane (dAM) holds significant potential in tissue engineering; however, its inherent mechanical limitations and rapid degradation hinder its clinical translation. This study integrates dAM with high molecular weight polymer polycaprolactone (PCL) and natural gelatin (Gel) nanofibers using electrospinning technology and a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) covalent crosslinking system to produce two composite biomaterials. Both PCL-dAM and Gel-dAM composites demonstrate enhanced strain, tensile strength, and elasticity compared to pure dAM, showcasing improved mechanical properties and significantly reduced degradation rates, with Gel-dAM exhibiting superior overall performance. Gel-dAM also shows considerably better compatibility with fibroblasts, macrophages, and tendon stem cells than PCL-dAM, suggesting that it more effectively supports cell adhesion, proliferation, and differentiation, thus providing a more favorable microenvironment for tissue repair. In macrophage immune modulation, Gel-dAM significantly promotes the polarization of macrophages toward the M2 phenotype, exhibiting potential anti-inflammatory and repair-enhancing effects, thereby offering new insights into the use of dAM in tissue regeneration. These advancements open new possibilities for the clinical application of dAM, particularly in tissue repair and wound dressing.
- Decellularized amniotic membrane,
- Electrospinning,
- Nanofiber,
- Polycaprolactone,
- Gelatin
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沈阳化工大学材料科学与工程学院沈阳 110142