Citation: Wei Zhang, Lei Yang, Zhihang Jin, Shusheng Zhang, Fawei Zhu, Zhanxian Li, Mingming Yu. Fluorescent probes reveal the differential impact of ferroptosis inhibition on drug-induced liver and kidney injury[J]. Chinese Chemical Letters, ;2026, 37(4): 111220. doi: 10.1016/j.cclet.2025.111220 shu

Fluorescent probes reveal the differential impact of ferroptosis inhibition on drug-induced liver and kidney injury

Wei Zhang ^a ,
Lei Yang ^b ,
Zhihang Jin ^a ,
Shusheng Zhang ^b ,
Fawei Zhu ^{b, *,} ,
Zhanxian Li ^{a, *,} ,
Mingming Yu ^{a, *,}

a.
College of Chemistry, Zhengzhou University, Zhengzhou 450001, China
b.
Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China

zhufawei@lyu.edu.cn

lizx@zzu.edu.cn

yumm@zzu.edu.cn

Received Date: 7 January 2025
Revised Date: 13 April 2025
Accepted Date: 15 April 2025
Available Online: 16 April 2025

Figures(6)

Ferroptosis has emerged as a significant pathway in various pathological conditions. Studying the effects of inhibiting ferroptosis on liver injury is instrumental in gaining a deeper understanding of the mechanisms. This study the design and synthesis of a multi-channel near-infrared emitting fluorescent probe TXVQ. When the probe TXVQ responds to HSO₃⁻, the fluorescence intensity at 500 nm of TXVQ increases with the addition of HSO₃⁻. As the concentration of H₂O₂ increases, the fluorescence intensity of TXVQ at 600 nm is enhanced. Concurrently, as viscosity rises, the fluorescence intensity of the probe TXVQ at 725 nm will gradually increase. The probe TXVQ, with its ability to monitor HSO₃⁻, H₂O₂ and viscosity through three distinct fluorescent channels, is advantageous for its application in the biological field. Subsequently, cellular experiments have demonstrated that the probe TXVQ can monitor changes in intracellular HSO₃⁻, H₂O₂ and viscosity. At the cellular level, it is found that inhibiting ferroptosis had no alleviating effect on drug-induced liver injury (DILI), but it had a certain alleviating effect on acute kidney injury (AKI). In a murine model, the effects of ferroptosis inhibition on DILI and AKI indicate that inhibiting ferroptosis reduced kidney injury but not liver injury, highlighting its potential in kidney therapeutics. TXVQ can detect various levels of HSO₃⁻, H₂O₂ and viscosity through three different fluorescent channels, making it a powerful tool for diagnosing and treating kidney diseases, as well as deepening the understanding of the role of ferroptosis in liver and kidney pathologies.
- Ferroptosis inhibition,
- Drug-induced liver injury,
- Kidney injury,
- HSO₃⁻,
- H₂O₂,
- Viscosity
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