Citation: Meitong Wu, Ke Wu, Shumin Feng, Li Xu, Mi Lei, Jianmei Chen, Shuang Li, Mian Qin, Dahui Liu, Guoqiang Feng. A NIR and ratiometric fluorescent probe for quantitative detection of SO₂ derivatives in Chinese medicinal materials and bioimaging in vivo[J]. Chinese Chemical Letters, ;2026, 37(1): 110979. doi: 10.1016/j.cclet.2025.110979 shu

A NIR and ratiometric fluorescent probe for quantitative detection of SO₂ derivatives in Chinese medicinal materials and bioimaging in vivo

Meitong Wu ^{a, 1} ,
Ke Wu ^{a, 1} ,
Shumin Feng ^{a, c, *,} ,
Li Xu ^a ,
Mi Lei ^a ,
Jianmei Chen ^a ,
Shuang Li ^a ,
Mian Qin ^a ,
Dahui Liu ^{a, c, *,} ,
Guoqiang Feng ^{b, *,}

a.
School of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China
b.
College of Chemistry, Central China Normal University, Wuhan 430079, China
c.
Hubei Shizhen Laboratory, Hubei University of Chinese Medicine, Wuhan 430065, China

fsm3318@hbucm.edu.cn

liudahui@hbucm.edu.cn

gf256@mail.ccnu.edu.cn

Received Date: 12 November 2024
Revised Date: 18 February 2025
Accepted Date: 19 February 2025
Available Online: 19 February 2025

Figures(5)

Sulfur dioxide (SO₂) and its derivatives have been recognized as harmful environmental pollutants. However, they are often produced during the processing of traditional Chinese medicines, potentially compromising the quality of these medicinal materials and contributing to various health issues. Due to a lack of effective monitoring and imaging tools, the physiological effects of excessive SO₂ residues in traditional Chinese medicine remain unclear. Therefore, developing a rapid and effective tool for detecting SO₂ is crucial for understanding its metabolic pathways and effects in vivo. In this study, we developed a near infrared (NIR) and ratiometric fluorescent probe, NIR-RS, which exhibits high sensitivity, selectivity, and rapid response for SO₂ detection. Notably, NIR-RS accurately quantifies SO₂ contents in Pinelliae rhizoma (P. rhizoma) samples, with recovery rates from 98.46% to 102.40%, and relative standard deviations (RSDs) < 5.0%. For bioimaging applications, NIR-RS has low cytotoxicity and good mitochondrial-targeting ability, making it suitable for imaging exogenous and endogenous SO₂ in mitochondria. Additionally, NIR-RS was successfully applied to image SO₂ content of P. rhizoma samples within cells, revealing that high SO₂ residue elevated mitochondria adenosine triphosphate (ATP) content, these findings reveal that P. rhizoma with excessive SO₂ can affect the organism's growth mechanisms through alterations in ATP pathways. In vivo, SO₂ was found to predominantly accumulate in the liver following gavage with P. rhizoma solution, with accumulation levels increasing in proportion to SO₂ residue concentration. High SO₂ concentrations in P. rhizoma can cause pulmonary fibrosis and gastric mucosal damage. This work provides a valuable tool for regulating SO₂ content in P. rhizoma and may help researcher better understand the metabolism of SO₂ derivatives and explore their physiological roles in biological systems.
- Fluorescent probe,
- SO₂ derivatives metabolism,
- Quantitative analysis,
- Pinelliae rhizoma,
- Fluorescent imaging
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A NIR and ratiometric fluorescent probe for quantitative detection of SO2 derivatives in Chinese medicinal materials and bioimaging in vivo

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通讯作者: 陈斌, bchen63@163.com

A NIR and ratiometric fluorescent probe for quantitative detection of SO₂ derivatives in Chinese medicinal materials and bioimaging in vivo