Citation: Qian Pang, Fangjun Huo, Yongkang Yue, Caixia Yin. ONOO⁻ and viscosity dual-response fluorescent probe for arthritis imaging in vivo[J]. Chinese Chemical Letters, ;2025, 36(9): 110713. doi: 10.1016/j.cclet.2024.110713 shu

ONOO⁻ and viscosity dual-response fluorescent probe for arthritis imaging in vivo

Qian Pang ^a ,
Fangjun Huo ^b ,
Yongkang Yue ^{a, *,} ,
Caixia Yin ^{a, *,}

a.
Institute of Molecular Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China
b.
Research Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China

ykyue@sxu.edu.cn

yincx@sxu.edu.cn

Received Date: 1 October 2024
Revised Date: 27 November 2024
Accepted Date: 3 December 2024
Available Online: 9 December 2024

Figures(7)

Rheumatoid arthritis (RA) is a chronic inflammatory disease with multi-system damage and autoimmune features. The main clinical manifestations of RA include joint pain, swelling, and stiffness, and RA may lead to joint deformity and dysfunction in severe cases. The pathologic development of RA involves complex interactions of multiple biomarkers, and detecting a single biomarker may produce false-positive results due to other confounding factors. Therefore, fluorescent probes that can detect multiple biomarkers simultaneously are crucial for precise RA diagnosis. Peroxynitrite (ONOO⁻) and viscosity are inflammation-related factors in cells. In this study, we developed a dual responsive near-infrared fluorescent probe, YLS, for ONOO⁻ and viscosity. The probe features dual-channel turn-on fluorescence responses at 625 and 760 nm upon the presence of ONOO⁻ and viscosity, respectively. Supported by YLS, we found that during RA pathology, lymphocyte infiltration not only increases the concentration of proteins in the joint fluid resulting in elevated viscosity; at the same time, the overproduction of ONOO⁻ exacerbates oxidative stress and inflammatory responses. This multiparameter assay is expected to improve the diagnostic accuracy of the early stages of RA, thus providing a scientific basis for early intervention and personalized treatment.
- Rheumatoid arthritis,
- ONOO⁻,
- Viscosity,
- Fluorescent probe,
- Dual-channel
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

ONOO− and viscosity dual-response fluorescent probe for arthritis imaging in vivo

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通讯作者: 陈斌, bchen63@163.com

ONOO⁻ and viscosity dual-response fluorescent probe for arthritis imaging in vivo