Citation: Shuo Tian, Shuyun Chen, Yunsen Wang, Dianping Tang. Liposomal photoelectrochemical immunoassay for low-abundance proteins with ternary transition metal sulfides for signal amplification[J]. Chinese Chemical Letters, ;2025, 36(7): 110418. doi: 10.1016/j.cclet.2024.110418 shu

Liposomal photoelectrochemical immunoassay for low-abundance proteins with ternary transition metal sulfides for signal amplification

Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, China

Corresponding author: Dianping Tang, dianping.tang@fzu.edu.cn
Received Date: 17 May 2024
Revised Date: 23 August 2024
Accepted Date: 5 September 2024
Available Online: 6 September 2024

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Development of accurate analytical protocols for cancer biomarkers is used for the initial prescreening of malignant tumors, disease surveillance, and efficacy assessment with significant clinical benefits. In this work, we reported a liposome-mediated signal-off photoelectrochemical (PEC) immunoassay for the sensitive detection of carcinoembryonic antigen (CEA) using ternary transition metal sulfide CuS/ZnCdS as the photoactive material. Good photocurrents were acquired on the basis of specific oxidation reaction of dopamine on the CuS/ZnCdS. The energy band relationship of CuS/ZnCdS was determined, and the well-matched oxidation potential of dopamine was verified. To achieve accurate recovery of low-abundance CEA, systematic PEC evaluation from human serum samples was performed by combining with classical immunoreaction and liposome-induced dopamine amplification strategy with high stability and selectivity. Under optimum conditions, PEC immunoassay displayed good photocurrent responses toward target CEA with a dynamic linear range of 0.1–50 ng/mL with a detection limit of 31.6 pg/mL. Importantly, this system by combining with a discussion of energy level matching between semiconductor energy bands and small-molecules opens a new horizon for development of high-efficient PEC immunoassays.
- Photoelectrochemical immunoassay,
- Liposome labelling,
- Carcinoembryonic antigen,
- Signal amplification,
- Ternary transition metal sulfides
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