Citation: Rui Li, Ruijie Lu, Libin Yang, Jianwen Li, Zige Guo, Qiquan Yan, Mengjun Li, Yazhuo Ni, Keying Chen, Yaoyang Li, Bo Xu, Mengzhen Cui, Zhan Li, Zhiying Zhao. Immobilization of chitosan nano-hydroxyapatite alendronate composite microspheres on polyetheretherketone surface to enhance osseointegration by inhibiting osteoclastogenesis and promoting osteogenesis[J]. Chinese Chemical Letters, ;2025, 36(4): 110242. doi: 10.1016/j.cclet.2024.110242 shu

Immobilization of chitosan nano-hydroxyapatite alendronate composite microspheres on polyetheretherketone surface to enhance osseointegration by inhibiting osteoclastogenesis and promoting osteogenesis

Rui Li ^{a, b, 1,*,} ,
Ruijie Lu ^{a, 1} ,
Libin Yang ^b ,
Jianwen Li ^c ,
Zige Guo ^a ,
Qiquan Yan ^a ,
Mengjun Li ^a ,
Yazhuo Ni ^a ,
Keying Chen ^a ,
Yaoyang Li ^a ,
Bo Xu ^a ,
Mengzhen Cui ^a ,
Zhan Li ^{d, *,} ,
Zhiying Zhao ^{e, *,}

a.
School and Hospital of Stomatology Tianjin Medical University, Tianjin 300070, China
b.
Department of Stomatology, Shizuishan Second People's Hospital, Shizuishan 753000, China
c.
Department of Radiological Shizuishan Second People's Hospital, Shizuishan 753000, China
d.
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China
e.
Baodi Hospital, Tianjin Medical University, Tianjin 301899, China

kqxiufulirui@tmu.edu.cn

zhanli590@gmail.com

809534649@qq.com

Received Date: 14 April 2024
Revised Date: 8 July 2024
Accepted Date: 11 July 2024
Available Online: 14 July 2024

Figures(4)

Polyetheretherketone (PEEK) is a desirable candidate to replace conventional metal implants owing to its excellent mechanical properties. However, the intrinsic bioinertness of PEEK results in inferior or delayed osseointegration, which limits its further clinical application. To address these challenges, one leading strategy is to construct a biofunctionalized surface on PEEK that provides a coordinated osteoblast-osteoclast interactions microenvironment. Herein, alendronate (ALN), a common bone absorption inhibitor, was loaded in biomedical inorganic/organic microspheres, consisting of bioactive inorganic nano-hydroxyapatite core, and chitosan (CS) shell. Polydopamine (PDA) modification was employed to ensure the adherence of the microspheres to the PEEK surface. The delivery of ALN and Ca²⁺ from these microspheres simultaneously suppressed osteoclastogenesis and promoted osteogenesis, resulting in a coordinated cascade of osteoblast-osteoclast interactions crucial for the per-implant osseointegration. In vitro experiments demonstrated that the PEEK surface exhibited satisfactory biocompatibility and enhanced the proliferation and osteogenic differentiation of rat bone mesenchymal stem cells while inhibiting the osteoclast differentiation. Moreover, the in vivo rat femoral drilling model demonstrated superior osseointegration three months after implantation. By considering the bone remodeling processes, this study proposes a novel biofunctionalized PEEK surface that regulates the activities of both osteoblasts and osteoclasts to promote osseointegration.
- Polyetheretherketone,
- Controlled drug delivery,
- Surface modification,
- Osseointegration,
- Osteoclasts
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