Citation: Yijian Zhao, Jvzhe Li, Yunyi Shi, Jie Hu, Meiyi Liu, Yao Shen, Xinglin Hou, Qiuyue Wang, Qi Wang, Zhiyi Yao. A label-free and ratiometric fluorescent sensor based on porphyrin-metal-organic frameworks for sensitive detection of ochratoxin A in cereal[J]. Chinese Chemical Letters, ;2025, 36(4): 110132. doi: 10.1016/j.cclet.2024.110132 shu

A label-free and ratiometric fluorescent sensor based on porphyrin-metal-organic frameworks for sensitive detection of ochratoxin A in cereal

Beijing Laboratory of Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

yaozy@cau.edu.cn

Received Date: 22 February 2024
Revised Date: 29 May 2024
Accepted Date: 14 June 2024
Available Online: 16 June 2024

Figures(5)

The fabrication of bioreceptor-free method for accurate and sensitive detection of ochratoxin A (OTA) in cereal is critical, but still a significant challenge to mitigate risks to food industries and public health. In this study, a smartphone-ratiometric fluorescence sensor for the ultrasensitive detection of OTA is developed based on a porphyrinic metal-organic framework and silica nanoparticle composite (Zr-MOF/SiNPs) away from the use of antibodies and aptamers. Due to the excellent recognition ability of Zr-MOF and good storage stability of SiNPs, OTA is detected by Zr-MOF/SiNPs with a wide linear range of 0.05–1000 ng/mL and low detection limit of 0.016 ng/mL. Moreover, the red–blue ratio values of the fluorescence images are extracted through the smartphone color recognizer application with a limit of detection of 1.74 ng/mL, lower than the permissible content of OTA in cereal prescribed by World Health Organization. This sensing platform has been successfully applied in maize samples with superior repeatability and satisfactory recoveries, providing a novel way for simple and label-free analysis of OTA in cereal.
- Ochratoxin A,
- Porphyrinic metal-organic framework,
- Silica nanoparticle,
- Ratiometric fluorescent sensing,
- Food safety
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