Citation: Meiling Zhao, Yao Lu, Yutao Zhang, Haoyun Xue, Zhiqian Guo. Ultra-high signal-to-noise ratio near-infrared chemiluminescent probe for in vivo sensing singlet oxygen[J]. Chinese Chemical Letters, ;2025, 36(5): 110105. doi: 10.1016/j.cclet.2024.110105 shu

Ultra-high signal-to-noise ratio near-infrared chemiluminescent probe for in vivo sensing singlet oxygen

State Key Laboratory of Bioreactor Engineering, Key Laboratory for Advanced Materials and Institute of Fine Chemicals, Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China

1539232010@qq.com

guozq@ecust.edu.cn

Received Date: 29 April 2024
Revised Date: 7 June 2024
Accepted Date: 11 June 2024
Available Online: 14 June 2024

Figures(5)

Singlet oxygen (¹O₂), as the primary reactive oxygen species in photodynamic therapy, can effectively induce excessive oxidative stress to ablate tumors and kill germs in clinical treatment. However, monitoring endogenous ¹O₂ is greatly challenging due to its extremely short lifetime and high reactivity in biological condition. Herein, we report an ultra-high signal-to-ratio near-infrared chemiluminescent probe (DCM-Cy) for the precise detection of endogenous ¹O₂ during photodynamic therapy (PDT). The methoxy moiety was removed from enolether unit in DCM-Cy to suppress the potential self-photooxidation reaction, thus greatly eliminating the photoinduced background signals during PDT. Additionally, the compact cyclobutane modification of DCM-Cy resulted in a significant 6-fold increase in cell permeability compared to conventional adamantane-dioxane probes. Therefore, our "step-by-step" strategy for DCM-Cy addressed the limitations of traditional chemiluminescent (CL) probes for ¹O₂, enabling effectively tracking of endogenous ¹O₂ level changes in living cells, pathogenic bacteria and mice in PDT.
- Fluorescent probe,
- Near-infrared,
- Chemiluminescent,
- Singlet oxygen,
- Bioimaging
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