Citation: Shaobin He, Xiaoyun Guo, Qionghua Zheng, Huanran Shen, Yuan Xu, Fenglin Lin, Jincheng Chen, Haohua Deng, Yiming Zeng, Wei Chen. Engineering nickel-supported osmium bimetallic nanozymes with specifically improved peroxidase-like activity for immunoassay[J]. Chinese Chemical Letters, ;2025, 36(4): 110096. doi: 10.1016/j.cclet.2024.110096 shu

Engineering nickel-supported osmium bimetallic nanozymes with specifically improved peroxidase-like activity for immunoassay

Shaobin He ^{a, b, e, 1} ,
Xiaoyun Guo ^{a, 1} ,
Qionghua Zheng ^a ,
Huanran Shen ^a ,
Yuan Xu ^c ,
Fenglin Lin ^d ,
Jincheng Chen ^b ,
Haohua Deng ^a ,
Yiming Zeng ^{c, e, *,} ,
Wei Chen ^{a, *,}

a.
Fujian Key Laboratory of Drug Target Discovery and Structural and Functional Research, School of Pharmacy, Fujian Medical University, Fuzhou 350004, China
b.
Laboratory of Clinical Pharmacy, Department of Pharmacy, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China
c.
Department of Pulmonary and Critical Care Medicine, The Second Affiliated Hospital of Fujian Medical University, Center of Respiratory Medicine of Fujian Province, Quanzhou 362000, China
d.
Department of Pharmacy, Quanzhou Women’s and Children’s Hospital, Quanzhou 362000, China
e.
Fujian Key Laboratory of Lung Stem Cells, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China

^**

zeng_yi_ming@126.com

chenandhu@163.com

Received Date: 5 February 2024
Revised Date: 28 April 2024
Accepted Date: 5 June 2024
Available Online: 5 June 2024

Figures(5)

Researchers have shown significant interest in modulating the peroxidase-like activity of nanozymes. Among these, bimetallic nanozymes have shown superior peroxidase-like activity over monometallic counterparts, offering enhanced performance and cost-efficiency in nanozyme designs. Herein, bimetallic nanozymes comprising nickel (Ni) and osmium (Os) incorporated into hyaluronate (HA) have been developed, resulting in HA-Ni_n/Os nanoclusters. Subsequently, comprehensive characterizations have been conducted. Further investigation has revealed that HA-Ni_n/Os efficiently catalyzed 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation with hydrogen peroxide (H₂O₂), confirming its peroxidase-like behavior and role as a nanozyme. Impressively, HA-Ni₂/Os (Ni/Os = 2:1) displays heightened substrate affinity, accelerated reaction rates, enhanced hydroxyl radical production in acidic conditions, and exhibits activity unit of 1224 U/mg, representing more than two-fold increase compared to non-Ni-supported Os nanozyme. Theoretical calculations indicate that Ni support enhances the peroxidase-like process of Os nanozyme by improving H₂O₂ adsorption and TMB oxidation. Crucially, the support of Ni does not significantly alter the other enzyme-like activities of Os nanozymes, thereby enabling Ni to selectively enhance their peroxidase-like activity. In terms of application, the peroxidase-like ability of HA-Ni₂/Os, facilitated by HA's carboxyl groups enabling crosslinking, proves effective in a squamous carcinoma antigen immunoassay. Moreover, HA-Ni₂/Os exhibit reliable stability, promising as a peroxidase substitute. This work underscores the advantages of incorporating Ni into Os, specifically enhancing peroxidase-like activity, highlighting the potential of Os bimetallic nanozymes for peroxidase-based applications.
- Nanozyme,
- Osmium,
- Nickel,
- Bimetallic,
- Peroxidase-like activity,
- Immunoassay
1. [1]
  P. Peng, C. Liu, Z. Li, et al., Trends Anal. Chem. 152 (2022) 116605.
2. [2]
  N.C. Veitch, Phytochemistry 65 (2004) 249–259. doi: 10.1016/j.phytochem.2003.10.022
3. [3]
  K. Sellami, A. Couvert, N. Nasrallah, et al., Sci. Total Environ. 806 (2022) 150500. doi: 10.1016/j.scitotenv.2021.150500
4. [4]
  L. Gao, J. Zhuang, L. Nie, et al., Nat. Nanotechnol. 2 (2007) 577–583. doi: 10.1038/nnano.2007.260
5. [5]
  H. Wei, E. Wang, Chem. Soc. Rev. 42 (2013) 6060–6093. doi: 10.1039/c3cs35486e
6. [6]
  J. Wu, X. Wang, Q. Wang, et al., Chem. Soc. Rev. 48 (2019) 1004–1076. doi: 10.1039/c8cs00457a
7. [7]
  G. Tang, J. He, J. Liu, X. Yan, K. Fan, Exploration 1 (2021) 75–89. doi: 10.1002/exp.20210005
8. [8]
  Y. Wang, Y. Xianyu, Small Methods 6 (2022) 2101576. doi: 10.1002/smtd.202101576
9. [9]
  K. Fan, J. Xi, L. Fan, et al., Nat. Commun. 9 (2018) 1440. doi: 10.1038/s41467-018-03903-8
10. [10]
  J. Xi, G. Wei, L. An, et al., Nano Lett. 19 (2019) 7645–7654. doi: 10.1021/acs.nanolett.9b02242
11. [11]
  J. Yang, R. Zhang, H. Zhao, et al., Exploration 2 (2022) 20210267. doi: 10.1002/EXP.20210267
12. [12]
  S.B. He, L.Y. Shi, Q.H. Zheng, et al., J. Mater. Chem. B 11 (2023) 7041–7054. doi: 10.1039/d3tb00930k
13. [13]
  Q. Zhao, D. Lu, G. Zhang, et al., Talanta 223 (2021) 121722.
14. [14]
  J. Lee, H. Liao, Q. Wang, et al., Exploration 2 (2022) 20210086. doi: 10.1002/EXP.20210086
15. [15]
  L. Jiao, W. Xu, Y. Zhang, Y. Wu, S. Guo, Nano Today 35 (2020) 100971. doi: 10.1016/j.nantod.2020.100971
16. [16]
  C. Shang, Q. Wang, H. Tan, et al., JACS Au 2 (2022) 2453–2459. doi: 10.1021/jacsau.2c00495
17. [17]
  Z. Wang, X. Shen, X. Gao, J. Phys. Chem. C 125 (2021) 23098–23104. doi: 10.1021/acs.jpcc.1c04878
18. [18]
  X. Niu, Q. Shi, W. Zhu, et al., Biosens. Bioelectron. 142 (2019) 111495. doi: 10.1016/j.bios.2019.111495
19. [19]
  Q. Liu, A. Zhang, R. Wang, Q. Zhang, D. Cui, Nano-Micro Lett. 13 (2021) 154.
20. [20]
  D. Pedone, M. Moglianetti, E. De Luca, G. Bardi, P.P. Pompa, Chem. Soc. Rev. 46 (2017) 4951–4975. doi: 10.1039/c7cs00152e
21. [21]
  S.B. He, L. Yang, M.T. Lin, et al., Biomed. Mater. 16 (2021) 032001.
22. [22]
  Z. Xi, X. Cheng, Z. Gao, et al., Nano Lett. 20 (2020) 272–277. doi: 10.1021/acs.nanolett.9b03782
23. [23]
  D. Wang, J. Wang, X.J. Gao, et al., Adv. Mater. 36 (2024) 2310033.
24. [24]
  S. He, L. Yang, P. Balasubramanian, S. Li, W. Chen, J. Mater. Chem. A 8 (2020) 25226–25234. doi: 10.1039/d0ta09247a
25. [25]
  S.B. He, M.T. Lin, L. Yang, et al., ACS Appl. Mater. Interfaces. 13 (2021) 44541–44548. doi: 10.1021/acsami.1c11907
26. [26]
  H.A.A. Noreldeen, L. Yang, X.Y. Guo, et al., Analyst 147 (2021) 101–108. doi: 10.1039/d1an01895g
27. [27]
  J. Pan, Q. He, Z. Lao, et al., Analyst 147 (2021) 55–65. doi: 10.32702/2306-6814.2021.17.55
28. [28]
  C. Tang, T. Fang, S. Chen, D. Zhang, J. Yin, H. Wang, Chem. Eng. J. 464 (2023) 142726.
29. [29]
  M. Luo, S. Guo, Nat. Rev. Mater. 2 (2017) 1–13.
30. [30]
  M. Luo, Z. Zhao, Y. Zhang, et al., Nature 574 (2019) 81–85. doi: 10.1038/s41586-019-1603-7
31. [31]
  M. Diez-Castellnou, F. Mancin, P. Scrimin, J. Am. Chem. Soc. 136 (2014) 1158–1161. doi: 10.1021/ja411969e
32. [32]
  M. Pietrzak, P. Ivanova, Sens. Actuators B 336 (2021) 129736. doi: 10.1016/j.snb.2021.12973
33. [33]
  X. Hai, Y. Zheng, Q. Yu, et al., Nature 622 (2023) 754–760. doi: 10.1038/s41586-023-06529-z
34. [34]
  Y. Hu, Z. Li, B. Li, C. Yu, Small 18 (2022) 2203589. doi: 10.1002/smll.202203589
35. [35]
  G.W. Wu, Y.M. Shen, X.Q. Shi, et al., Anal. Chim. Acta 971 (2017) 88–96.
36. [36]
  Z. Xi, K. Wei, Q. Wang, et al., J. Am. Chem. Soc. 143 (2021) 2660–2664. doi: 10.1021/jacs.0c12605
37. [37]
  Y. Tang, Y. Chen, Y. Wu, et al., Nano Lett. 23 (2022) 267–275. doi: 10.1021/acs.nanolett.2c04235
38. [38]
  Y. Zhu, R. Zhao, L. Feng, et al., ACS Nano 17 (2023) 6833–6848. doi: 10.1021/acsnano.3c00423
39. [39]
  B. Jiang, D. Duan, L. Gao, et al., Nat. Protoc. 13 (2018) 1506–1520. doi: 10.1038/s41596-018-0001-1
40. [40]
  J. Li, W. Liu, X. Wu, X. Gao, Biomaterials 48 (2015) 37–44. doi: 10.3991/ijoe.v11i3.4488
41. [41]
  C. Ge, G. Fang, X. Shen, et al., ACS Nano 10 (2016) 10436–10445. doi: 10.1021/acsnano.6b06297
42. [42]
  X. Wang, X.J. Gao, L. Qin, et al., Nat. Commun. 10 (2019) 704. doi: 10.1038/s41467-019-08657-5
43. [43]
  I. Letchumanan, S.C.B. Gopinath, M.K.M. Arshad, Mikrochim. Acta 187 (2020) 1–9. doi: 10.1007/s00604-019-3921-8
44. [44]
  G. Mo, D. Qin, X. Jiang, et al., Sens. Actuators B 310 (2020) 127852.
45. [45]
  Y.W. Mao, J.X. Zhang, D.N. Chen, A.J. Wang, J.J. Feng, Sens. Actuators B 370 (2022) 132416.
46. [46]
  X. Ren, D. Wu, Y. Wang, et al., Biosens. Bioelectron. 72 (2015) 156–159. doi: 10.1016/j.bios.2015.05.012
47. [47]
  H. Kumar, N. Kumari, R. Sharma, Environ. Impact Assess. Rev. 85 (2020) 106438. doi: 10.1016/j.eiar.2020.106438
48. [48]
  J. Langer, D.J. Aberasturi, J. Aizpurua, et al., ACS Nano 14 (2020) 28–117. doi: 10.1021/acsnano.9b04224
49. [49]
  A.M. Ashrafi, Z. Bytesnikova, J. Barek, L. Richtera, V. Adam, Biosens. Bioelectron. 192 (2021) 113494. doi: 10.1016/j.bios.2021.113494
1. [1]
  Jiangping Chen , Hongju Ren , Kai Wu , Huihuang Fang , Chongqi Chen , Li Lin , Yu Luo , Lilong Jiang . Boosting hydrogen production of ammonia decomposition via the construction of metal-oxide interfaces. Chinese Journal of Structural Chemistry, 2024, 43(2): 100236-100236. doi: 10.1016/j.cjsc.2024.100236
2. [2]
  Zhenkang Ai , Hui Chen , Xuebin Liao . Nickel-catalyzed decarboxylative difluoromethylation and alkylation of alkenes. Chinese Chemical Letters, 2025, 36(3): 109954-. doi: 10.1016/j.cclet.2024.109954
3. [3]
  Ying Wang , Hong Yang , Caixia Zhu , Qing Hong , Xuwen Cao , Kaiyuan Wang , Yuan Xu , Yanfei Shen , Songqin Liu , Yuanjian Zhang . Cascading oxidoreductases-like nanozymes for high selective and sensitive fluorescent detection of ascorbic acid. Chinese Chemical Letters, 2025, 36(4): 110153-. doi: 10.1016/j.cclet.2024.110153
4. [4]
  Jia Chen , Yun Liu , Zerong Long , Yan Li , Hongdeng Qiu . Colorimetric detection of α-glucosidase activity using Ni-CeO₂ nanorods and its application to potential natural inhibitor screening. Chinese Chemical Letters, 2024, 35(9): 109463-. doi: 10.1016/j.cclet.2023.109463
5. [5]
  Xiao-Bo Liu , Ren-Ming Liu , Xiao-Di Bao , Hua-Jian Xu , Qi Zhang , Yu-Feng Liang . Nickel-catalyzed reductive formylation of aryl halides via formyl radical. Chinese Chemical Letters, 2024, 35(12): 109783-. doi: 10.1016/j.cclet.2024.109783
6. [6]
  Xinghao Cai , Chen Ma , Ying Kang , Yuqiang Ren , Xue Meng , Wei Lu , Shiming Fan , Shouxin Liu . Nickel-catalyzed C(sp²)–H alkynylation of free α-substituted benzylamines using a transient directing group. Chinese Chemical Letters, 2025, 36(10): 110901-. doi: 10.1016/j.cclet.2025.110901
7. [7]
  Caixia Zhu , Qing Hong , Kaiyuan Wang , Yanfei Shen , Songqin Liu , Yuanjian Zhang . Single nanozyme-based colorimetric biosensor for dopamine with enhanced selectivity via reactivity of oxidation intermediates. Chinese Chemical Letters, 2024, 35(10): 109560-. doi: 10.1016/j.cclet.2024.109560
8. [8]
  Xiaoshuai Wu , Bailei Wang , Yichen Li , Xiaoxuan Guan , Mingjing Yin , Wenquan Lv , Yin Chen , Fei Lu , Tao Qin , Huyang Gao , Weiqian Jin , Yifu Huang , Cuiping Li , Ming Gao , Junyu Lu . NIR driven catalytic enhanced acute lung injury therapy by using polydopamine@Co nanozyme via scavenging ROS. Chinese Chemical Letters, 2025, 36(2): 110211-. doi: 10.1016/j.cclet.2024.110211
9. [9]
  Zhirong Yang , Shan Wang , Ming Jiang , Gengchen Li , Long Li , Fangzhi Peng , Zhihui Shao . One stone three birds: Ni-catalyzed asymmetric allenylic substitution of allenic ethers, hydroalkylation of 1,3-enynes and double alkylation of enynyl ethers. Chinese Chemical Letters, 2024, 35(8): 109518-. doi: 10.1016/j.cclet.2024.109518
10. [10]
  Mingyue Luo , Kehui Zhang , Honghong Rao , Jianying Li , Xin Xue , Panpan Sun , Xiaoquan Lu , Zhonghua Xue . A simplified ratiometric fluorescent sensing strategy for enhanced detection of alkaline phosphatase employing Prussian blue nanozymes and commercially available chromogen. Chinese Chemical Letters, 2025, 36(9): 110703-. doi: 10.1016/j.cclet.2024.110703
11. [11]
  Manoj Kumar Sarangi , L․D Patel , Goutam Rath , Sitansu Sekhar Nanda , Dong Kee Yi . Metal organic framework modulated nanozymes tailored with their biomedical approaches. Chinese Chemical Letters, 2024, 35(11): 109381-. doi: 10.1016/j.cclet.2023.109381
12. [12]
  Jiaxi Wang , Zhiwei Gao , Hao Liang , Qianyue Liu , Weiqian Jin , Huyang Gao , Bailei Wang , Ruikai Zhu , Jiahao Huang , Xiaowen Li , Xingmou Wu , Weijiu Mo , Yinhan Liao , Ming Gao , Xiaojie Li , Cuiping Li . NIR stimulated epigallocatechin gallate loaded polydopamine with enhanced antibacterial and ROS scavenging abilities for improved infectious wound healing. Chinese Chemical Letters, 2025, 36(7): 110569-. doi: 10.1016/j.cclet.2024.110569
13. [13]
  Guanghui Lin , Jieyao Chen , Xiaojia Liu , Yitong Lin , Xudong Zhu , Guotao Yuan , Bowen Yang , Shuanshuan Guo , Yue Pan , Jianhua Zhou . Sustained modulation of tumor microenvironment via sorafenib-loaded mesoporous ferromanganese nanozymes for enhanced apoptosis-ferroptosis cancer therapy. Chinese Chemical Letters, 2025, 36(8): 111018-. doi: 10.1016/j.cclet.2025.111018
14. [14]
  Yuwan Lu , Xiaodan Zhang , Yuming Huang . Dual-site Se/NC specific peroxidase-like nanozyme for highly sensitive methimazole detection. Chinese Chemical Letters, 2025, 36(4): 110129-. doi: 10.1016/j.cclet.2024.110129
15. [15]
  Ruixin Liu , Feng Shi , Yanping Xia , Haibing Zhu , Jiawen Cao , Kai Peng , Chuanli Ren , Juan Li , Zhanjun Yang . Universal MOF nanozyme-induced catalytic amplification strategy for label-free electrochemical immunoassay. Chinese Chemical Letters, 2024, 35(11): 109664-. doi: 10.1016/j.cclet.2024.109664
16. [16]
  Hui Yang , Guangxun Zhang , Yueyao Sun , Huijie Zhou , Huan Pang . Bimetallic zeolitic imidazolate framework derived hollow layered double hydroxide with tailorable interlayer spacing for nickel-zinc batteries. Chinese Chemical Letters, 2025, 36(6): 110016-. doi: 10.1016/j.cclet.2024.110016
17. [17]
  Simin Wei , Yaqing Yang , Junjie Li , Jialin Wang , Jinlu Tang , Ningning Wang , Zhaohui Li . The Mn/Yb/Er triple-doped CeO₂ nanozyme with enhanced oxidase-like activity for highly sensitive ratiometric detection of nitrite. Chinese Chemical Letters, 2024, 35(6): 109114-. doi: 10.1016/j.cclet.2023.109114
18. [18]
  Peng Jin , Sili Lin , Dongmei Wang , Jinsong Fan , Qingyun Liu , Kun Li . Valence-band hybridization endows the reaction specificity of AgPd nanozyme for exclusive peroxidase mimicking and improved sensing performance. Chinese Chemical Letters, 2025, 36(11): 110916-. doi: 10.1016/j.cclet.2025.110916
19. [19]
  Yu Dai , Xueting Sun , Haoyu Wu , Naizhu Li , Guoe Cheng , Xiaojin Zhang , Fan Xia . Determination of the Michaelis Constant for Gold Nanozyme-Catalyzed Decomposition of Hydrogen Peroxide. University Chemistry, 2025, 40(5): 351-356. doi: 10.12461/PKU.DXHX202407052
20. [20]
  Yang Li , Jiachen Li , Daidi Fan . 二硫化钼纳米片的制备及其纳米酶性能探究——介绍一个大学化学综合实验. University Chemistry, 2025, 40(8): 233-240. doi: 10.12461/PKU.DXHX202410016

Get Citation

PDF

XML

Metrics

PDF Downloads(3)
Abstract views(984)
HTML views(24)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142