Citation: Wei-Tao Dou, Qing-Wen Zeng, Yan Kang, Haidong Jia, Yulian Niu, Jinglong Wang, Lin Xu. Construction and application of multicomponent fluorescent droplets[J]. Chinese Chemical Letters, ;2025, 36(1): 109995. doi: 10.1016/j.cclet.2024.109995 shu

Construction and application of multicomponent fluorescent droplets

Wei-Tao Dou ^{a, c} ,
Qing-Wen Zeng ^a ,
Yan Kang ^{b, *,} ,
Haidong Jia ^b ,
Yulian Niu ^b ,
Jinglong Wang ^b ,
Lin Xu ^{a, *,}

a.
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Shanghai Frontiers Science Center of Molecule Intelligent Syntheses, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200062, China
b.
Shanghai Jahwa United Co., Ltd., Shanghai 200082, China
c.
Chongqing Key Laboratory of Precision Optics, Chongqing Institute of East China Normal University, Chongqing 401120, China

kangyan@jahwa.com.cn

lxu@chem.ecnu.edu.cn

Received Date: 30 November 2023
Revised Date: 7 May 2024
Accepted Date: 10 May 2024
Available Online: 11 May 2024

Figures(11)

The rapid development of microfluidic technology has led to the evolution of microdroplets from simple emulsion structures to complex multilayered and multicompartmental configurations. These advancements have endowed microdroplets with the capability to contain multiple compartments that remain isolated from one another, enabling them to carry different molecules of interest. Consequently, researchers can now investigate intricate spatially confined chemical reactions and signal transduction pathways within subcellular organelles. Moreover, modern microdroplets often possess excellent optical transparency, allowing fluorescently labelled, multi-layered, and compartmental droplets to provide detailed insights through real-time, in situ, and dynamic fluorescence imaging. Hence, this review systematically summarizes current methodologies for preparing multicomponent microdroplets and their applications, particularly focusing on fluorescent microdroplets. Additionally, it discusses existing critical challenges and outlines future research directions. By offering a comprehensive overview of the preparation methods and applications of fluorescent microdroplets, this review aims to stimulate the interest of researchers and foster their utilization in more complex and biomimetic environments.
- Fluorescent,
- Microfluidic,
- Fluorescent droplets,
- Multilayered droplets,
- Multicompartmental droplets
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