Citation: Zhaorui Song, Qiulian Hao, Bing Li, Yuwei Yuan, Shanshan Zhang, Yongkuan Suo, Hai-Hao Han, Zhen Cheng. NIR-Ⅱ fluorescence lateral flow immunosensor based on efficient energy transfer probe for point-of-care testing of tumor biomarkers[J]. Chinese Chemical Letters, ;2025, 36(1): 109834. doi: 10.1016/j.cclet.2024.109834 shu

NIR-Ⅱ fluorescence lateral flow immunosensor based on efficient energy transfer probe for point-of-care testing of tumor biomarkers

Zhaorui Song ^{a, 1} ,
Qiulian Hao ^{a, 1} ,
Bing Li ^c ,
Yuwei Yuan ^d ,
Shanshan Zhang ^a ,
Yongkuan Suo ^{a, b, e, *,} ,
Hai-Hao Han ^{a, b, d, e, *,} ,
Zhen Cheng ^{a, b, c, e, *,}

a.
Shandong Laboratory of Yantai Drug Discovery, Bohai Rim Advanced Research Institute for Drug Discovery, Yantai 264117, China
b.
State Key Laboratory of Drug Research, Molecular Imaging Center, Shanghai Institute of Materia Medica, Shanghai 201203, China
c.
Institute of Molecular Medicine Joint Laboratory for Molecular Medicine Northeastern University, Shenyang 110000, China
d.
Pharmacy College, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji'nan 250117, China
e.
University of Chinese Academy of Sciences, Beijing 100049, China

suoyongkuan@simm.ac.cn

hanhaihao@simm.ac.cn

zcheng@simm.ac.cn

Received Date: 23 November 2023
Revised Date: 26 February 2024
Accepted Date: 25 March 2024
Available Online: 26 March 2024

Figures(4)

Fluorescence lateral flow immunoassay (LFA) has emerged as a powerful tool for rapid screening of various biomarkers owing to its simplicity, sensitivity and flexibility. It is noteworthy that fluorescent probe mainly determines the analytical performance of LFA. Due to the emission and excitation wavelengths are located in the visible region, most fluorophores are inevitably subject to light scattering and background autofluorescence. Herein, we reported a novel LFA sensor based on the second near-infrared (NIR-Ⅱ) fluorescent probe with excellent anti-interference capability. The designed NIR-Ⅱ probe was the Nd³⁺ and Yb³⁺ doped rare earth nanoparticles (RENPs) by employing Nd³⁺ as energy donor and Yb³⁺ as energy acceptor, which of the donor-acceptor energy transfer (ET) efficiency reached up to 80.7%. Meanwhile, relying on the convenient and effective encapsulation strategy of poly(lactic-co-glycolic acid) (PLGA) microspheres to RENPs, the surface functionalized NIR-Ⅱ probe (RE@PLGA) was obtained for subsequent bioconjugation. Benefiting from the optical advantages of NIR-Ⅱ probe, this proposed NIR-Ⅱ LFA displayed a good linear relationship ranging from 7 ng/mL to 200 ng/mL for the detection of α-fetoprotein (AFP), an important biomarker of hepatocellular carcinoma (HCC). The limit of detection (LOD) was determined as low as 3.0 ng/mL, which was of 8.3 times lower than clinical cutoff value. It is promising that LFA sensor based on this efficient RENPs probe provides new opportunities for high sensitive detection of various biomarkers in biological samples.
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