Citation: Yu-Qi Cao, Ying-Jie Lu, Li Zhang, Jing Zhang, Yin-Long Guo. Vacuum promoted on-tissue derivatization strategy: Unravelling spatial distribution of glycerides on tissue[J]. Chinese Chemical Letters, ;2024, 35(12): 109788. doi: 10.1016/j.cclet.2024.109788 shu

Vacuum promoted on-tissue derivatization strategy: Unravelling spatial distribution of glycerides on tissue

Yu-Qi Cao ^{a, b, 1} ,
Ying-Jie Lu ^{a, c, 1} ,
Li Zhang ^a ,
Jing Zhang ^{a, *,} ,
Yin-Long Guo ^{a, *,}

a.
State Key Laboratory of Organometallic Chemistry and National Center for Organic Mass Spectrometry in Shanghai, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200032, China
b.
Technical Centre, Shanghai Tobacco (Group) Corp., Shanghai 200082, China
c.
Department of Pharmacognosy, School of Pharmacy, Naval Medical University, Shanghai 200433, China

zhangjing@sioc.ac.cn

ylguo@sioc.ac.cn

Received Date: 3 December 2023
Revised Date: 19 February 2024
Accepted Date: 18 March 2024
Available Online: 19 March 2024

Figures(7)

Matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) has shown its capability in visualizing the spatial distribution of various kinds of endogenous metabolites. Nevertheless, high quality mass imaging of low polar metabolites remains challenging. Herein, a platform for sensitive matrix-assisted laser desorption ionization-mass spectrometry imaging of cholesterol and glycerides has been proposed. In the platform, a vacuum promoted on-tissue derivatization strategy was proposed to constantly make the derivatization reaction proceed towards to the direction of products. Compared with traditional on-tissue derivatization procedure, the strategy improved the acquired intensity of derivatized glycerides about 50%. Additionally, the mass spectrometry image reflecting the signal ratio between 3 classes of glycerides was achieved to exploit the metabolic level of glycerides on tissue slice. Finally, the platform was applied to brain slices of Alzheimer's transgenic mice, type 2 diabetes mice and normal mice. Significant difference was found in mass spectrometry images reflecting the signal ratio of multiple endogenous metabolites. The work constructed a promising platform for mapping of glycerides in tissue by mass spectrometry imaging.
- Mass spectrometry imaging,
- On-tissue derivatization,
- Glycerides,
- Alzheimer's disease
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