Citation: Miao-Miao Chen, Min-Ling Zhang, Xiao Song, Jun Jiang, Xiaoqian Tang, Qi Zhang, Xiuhua Zhang, Peiwu Li. Smartphone-assisted electrochemiluminescence imaging test strips towards dual-signal visualized and sensitive monitoring of aflatoxin B1 in corn samples[J]. Chinese Chemical Letters, ;2025, 36(1): 109785. doi: 10.1016/j.cclet.2024.109785 shu

Smartphone-assisted electrochemiluminescence imaging test strips towards dual-signal visualized and sensitive monitoring of aflatoxin B1 in corn samples

Miao-Miao Chen ^{a, b, e} ,
Min-Ling Zhang ^e ,
Xiao Song ^e ,
Jun Jiang ^{a, b} ,
Xiaoqian Tang ^{a, b, c, d} ,
Qi Zhang ^{a, b, c, d} ,
Xiuhua Zhang ^e ,
Peiwu Li ^{a, b, c, d, *,}

a.
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs; Laboratory of Risk Assessment for Oilseed Products (Wuhan), Quality Inspection and Test Center for Oilseed Products, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
b.
Food Safety Research Institute, Hubei University, Wuhan 430062, China
c.
Hubei Hongshan Laboratory, Wuhan 430070, China
d.
Xianghu Laboratory, Hangzhou 311231, China
e.
College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China

peiwuli@oilcrops.cn

Received Date: 25 February 2024
Revised Date: 17 March 2024
Accepted Date: 17 March 2024
Available Online: 18 March 2024

Figures(5)

Aflatoxins B1 (AFB1) contamination in agro-food holds great threaten to human and animal health. Conventional test strips for rapid AFB1 visualized monitoring remains challenged by improvement of sensitivity and matrix interference resistance. In this case, we developed a portable electrochemiluminescence (ECL) imaging test strip with dual-signal outputs for AFB1 quantification in corn samples. Ru-PEI@SiO₂@Au nanospheres were synthesized for bonding with anti-AFB1 antibody and then colorimetrical signal-reported on test line through the capillary flow at strips. Meanwhile, ECL imaging signal of the constructed carbon-ink-based working electrode on polyvinyl chloride substrate of strips was exported under an applied potential of 1.25 V. The whole ECL test strips not only endowed convenient colorimetric responses but guaranteed quick-witted ECL image distinguishment even at extremely low AFB1 content. The detection limit of this ECL imaging-integrated mode was 10-fold lower than that of only colorimetric mode. Furthermore, satisfactory selectivity, reliability and practicability of the as-proposed ECL test strips were demonstrated. This work offered a promising platform for on-site, accurate and sensitive detection of pollutants in foods.
- Electrochemiluminescence imaging,
- Test strips,
- Nanomaterials,
- Dual-signal outputs,
- Aflatoxin B1,
- Food safety
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