Citation: Caixia Zhu, Qing Hong, Kaiyuan Wang, Yanfei Shen, Songqin Liu, Yuanjian Zhang. Single nanozyme-based colorimetric biosensor for dopamine with enhanced selectivity via reactivity of oxidation intermediates[J]. Chinese Chemical Letters, ;2024, 35(10): 109560. doi: 10.1016/j.cclet.2024.109560 shu

Single nanozyme-based colorimetric biosensor for dopamine with enhanced selectivity via reactivity of oxidation intermediates

Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Devices, Jiangsu Province Hi-Tech Key Laboratory for Bio-Medical Research, School of Chemistry and Chemical Engineering, Medical School, Southeast University, Nanjing 211189, China

Yanfei.Shen@seu.edu.cn

Yuanjian.Zhang@seu.edu.cn

Received Date: 3 November 2023
Revised Date: 4 January 2024
Accepted Date: 22 January 2024
Available Online: 1 February 2024

Figures(3)

Reliable and selective sensing of dopamine (DA) is essential for early diagnosis of mental diseases. Among the various potential methods, nanozyme-based sensing systems have demonstrated promising sensitivity and reliability. However, owing to the lack of substrate specificity, it is challenging to selectively detect DA using nanozymes. Herein, based on the reactivity of the DA oxidation intermediates, we report a cascade colorimetric sensing system for the selective detection of DA using only a single nanozyme. It was disclosed that the oxidation product of DA catalyzed by Co-N-doped carbon sheets (Co-N-C, a common oxidase-like nanozyme), dopamine quinone (DAQ), showed significant biocatalytic electron-donating activity in the reduction of O₂ to generate O₂^•−. Further using O₂^•− to oxidize 3,3′,5,5′-tetramethylbenzidine (TMB), a colorimetric sensing platform for DA was constructed with a linear detection range of 50 nmol/L to 50 µmol/L and a low limit of detection of 4 nmol/L. Thanks to the reactivity of the oxidation product, without any biometric units (such as nucleic acids, enzymes, and antibodies/antigens), the reaction selectivity of DA against other interferences (e.g., ascorbic acid, adrenaline, 5-hydroxytryptamine, and glutathione) was enhanced up to 71-fold. Beyond complicated cascade systems requiring at least two nanozymes, sophisticated artificial recognition via multiple interactions was simplified by exploiting the oxidative properties of product intermediates; thus, only a single common oxidase-like nanozyme was needed. This work offers a new strategy to enhance the selectivity of nanozymes for bioanalytical applications.
- Nanozyme,
- Colorimetry,
- Selectivity,
- Dopamine,
- Artificial recognition
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