Citation: Ying Gao, Rong Zhou, Qiwen Wang, Shaolong Qi, Yuanyuan Lv, Shuang Liu, Jie Shen, Guocan Yu. Natural killer cell membrane doped supramolecular nanoplatform with immuno-modulatory functions for immuno-enhanced tumor phototherapy[J]. Chinese Chemical Letters, ;2024, 35(10): 109521. doi: 10.1016/j.cclet.2024.109521 shu

Natural killer cell membrane doped supramolecular nanoplatform with immuno-modulatory functions for immuno-enhanced tumor phototherapy

Ying Gao ^{a, b} ,
Rong Zhou ^{a, b} ,
Qiwen Wang ^{c, *,} ,
Shaolong Qi ^d ,
Yuanyuan Lv ^{a, b} ,
Shuang Liu ^{e, *,} ,
Jie Shen ^{a, b, *,} ,
Guocan Yu ^{d, *,}

a.
Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
b.
Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang Province, School of Medicine, Hangzhou City University, Hangzhou 310015, China
c.
Department of Cardiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
d.
MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China
e.
Emergency Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100730, China

wangqiwen@zju.edu.cn

liushuang80@pumch.cn

shenj@zucc.edu.cn

guocanyu@mail.tsinghua.edu.cn

Received Date: 23 November 2023
Revised Date: 8 January 2024
Accepted Date: 15 January 2024
Available Online: 20 January 2024

Figures(5)

Photodynamic therapy (PDT) has garnered significant attention as a promising approach to cancer therapy, harnessing the combined benefits of localized light treatment and the accompanying host immune response. In this study, we engineered an immuno-enhanced PDT nanoplatform, denoted as HM@p-MOF (hybrid membrane@porphyrin-metal organic framework). The core porphyrin-MOF was cloaked with a hybrid membrane derived from B16F10 cancer cells and NK cells, resulting in enhanced stability. In both in vitro and in vivo experiments, our finding demonstrated that the hybrid membrane conferred dual targeting capabilities to the nanoplatform, leveraging the unique properties of the B16F10 membrane and NK membrane to augment immunogenic cell death (ICD) induced by photodynamic effects. Additionally, in conjunction with the immunomodulatory functions of the NK cell membrane, we observed an expansion of in situ immune infiltration leading to a systemic immune response. The HM@p-MOF nanoplatform exhibited the capacity to not only inhibit the growth of mouse melanoma but also suppress metastasis. This innovative HM@p-MOF nanoplatform present a viable strategy to enhance phototherapeutic efficacy for both localized and metastatic tumors. It provides a direction for the fabrication of biomimetic nanomedicines possessing immuno-modulatory function.
- Biomimetic,
- Cell membrane,
- Metal-organic framework,
- Phototherapy,
- Tumor targeting
1. [1]
  Y. Xia, L. Rao, H. Yao, et al., Adv. Mater. 32 (2020) 2002054. doi: 10.1002/adma.202002054
2. [2]
  S. Cheng, C. Xu, Y. Jin, et al., Adv. Sci. 7 (2020) 1903301. doi: 10.1002/advs.201903301
3. [3]
  E. Vivier, E. Tomasello, M. Baratin, et al., Nat. Immunol. 9 (2008) 503–510. doi: 10.1038/ni1582
4. [4]
  G. Deng, Z. Sun, S. Li, et al., ACS Nano 12 (2018) 12096–12108. doi: 10.1021/acsnano.8b05292
5. [5]
  M.J. Smyth, Y. Hayakawa, K. Takeda, et al., Nat. Rev. Cancer 2 (2002) 850–861. doi: 10.1038/nrc928
6. [6]
  D. Piccioli, S. Sbrana, E. Melandri, et al., J. Exp. Med. 195 (2002) 335–341. doi: 10.1084/jem.20010934
7. [7]
  L. Chiossone, P.Y. Dumas, M. Vienne, et al., Nat. Rev. Immunol. 18 (2018) 671–688. doi: 10.1038/s41577-018-0061-z
8. [8]
  A. Diefenbach, A.M. Jamieson, S.D. Liu, et al., Nat. Immunol. 1 (2000) 119–126. doi: 10.1038/77793
9. [9]
  G. Deng, X. Peng, Z. Sun, et al., ACS Nano 14 (2020) 11452–11462. doi: 10.1021/acsnano.0c03824
10. [10]
  D.E.J.G.J. Dolmans, D. Fukumura, R.K. Jain, Nat. Rev. Cancer 3 (2003) 380–387. doi: 10.1038/nrc1071
11. [11]
  L. Benov, Med. Princ. Pract. 24 (2015) 14–28. doi: 10.1159/000362416
12. [12]
  L.D. Yue, K.K. Yang, J.W. Wei, et al., CCS Chem. 4 (2022) 1745–1757. doi: 10.31635/ccschem.021.202101029
13. [13]
  M.L. Agarwal, H.E. Larkin, S.I. Zaidi, et al., Cancer Res. 53 (1993) 5897–5902.
14. [14]
  W.J. de Vree, A.N. Fontijne-Dorsman, J.F. Koster, et al., Br. J. Cancer 73 (1996) 1335–1340. doi: 10.1038/bjc.1996.255
15. [15]
  D.V. Krysko, A.D. Garg, A. Kaczmarek, et al., Nat. Rev. Cancer 12 (2012) 860–875. doi: 10.1038/nrc3380
16. [16]
  X.P. Duan, C. Chan, W.B. Lin, Angew. Chem. Int. Ed. 58 (2019) 670–680. doi: 10.1002/anie.201804882
17. [17]
  R.H. Fang, A.V. Kroll, W. Gao, et al., Adv. Mater. 30 (2018) 1706759. doi: 10.1002/adma.201706759
18. [18]
  B. Zhang, L. Lin, J. Mao, et al., Chin. Chem. Lett. 34 (2023) 108518. doi: 10.1016/j.cclet.2023.108518
19. [19]
  X.F. Bai, Y. Chen, M.Z. Zou, et al., ACS Nano 16 (2022) 18555–18567. doi: 10.1021/acsnano.2c06871
20. [20]
  H.T. Zhang, R. Peng, S. Chen, A. Shen, et al., Adv. Sci. 9 (2022) e2202039. doi: 10.1002/advs.202202039
21. [21]
  W. Hao, Y. Cui, Y. Fan, M. Chen, et al., J. Nanobiotechnology 19 (2021) 378. doi: 10.1186/s12951-021-01110-0
22. [22]
  C. Gao, Q. Cheng, J.Y. Li, et al., Adv. Funct. Mater. 31 (2021) 2102440. doi: 10.1002/adfm.202102440
23. [23]
  C. Gao, C.H.T. Kwong, Q.F. Wang, et al., ACS Nano 17 (2023) 4034–4049. doi: 10.1021/acsnano.3c00041
24. [24]
  C. Xu, Y. Jiang, Y. Han, et al., Adv. Mater. 33 (2021) 2008061. doi: 10.1002/adma.202008061
25. [25]
  X. Liu, X. Zhong, C. Li, Chin. Chem. Lett. 32 (2021) 2347–2358. doi: 10.1016/j.cclet.2021.03.015
26. [26]
  J. Xiong, M. Wu, J. Chen, et al., ACS Nano 15 (2021) 19756–19770. doi: 10.1021/acsnano.1c07180
27. [27]
  L. Rao, L. Wu, Z. Liu, et al., Nat. Comm. 11 (2020) 4909. doi: 10.1038/s41467-020-18626-y
28. [28]
  Y. Zhang, Y. Jiao, X. Jia, et al., Chin. Chem. Lett. 35 (2024) 108748. doi: 10.1016/j.cclet.2023.108748
29. [29]
  J. Park, Q. Jiang, D. Feng, et al., J. Am. Chem. Soc. 138 (2016) 3518–3525. doi: 10.1021/jacs.6b00007
30. [30]
  A.D. Garg, D.V. Krysko, T. Verfaillie, et al., EMBO J. 31 (2012) 1062–1079. doi: 10.1038/emboj.2011.497
1. [1]
  Yuanzheng Wang , Chen Zhang , Shuyan Han , Xiaoli Kong , Changyun Quan , Jun Wu , Wei Zhang . Cancer cell membrane camouflaged biomimetic gelatin-based nanogel for tumor inhibition. Chinese Chemical Letters, 2024, 35(11): 109578-. doi: 10.1016/j.cclet.2024.109578
2. [2]
  Zhiqiang Cui , Dongxiang Zhang , Yanru Huang , Chunyu Shao , Yiming Zhang , Changliang Sun , Xin-Dong Jiang , Xiaohong Sun , Qinghuan Xiao . Novel 1-phenylethyl-containing aza-BDOIPY for phototherapy and simultaneous monitoring of tumor immune microenvironment reprogramming. Chinese Chemical Letters, 2025, 36(7): 110460-. doi: 10.1016/j.cclet.2024.110460
3. [3]
  Haotian Shi , Yuchao Luo , Song Zhang , Meijun Zhao , Chaoyong Liu , Qing Pei , Helei Wang , Qiong Dai , Zhigang Xie , Bin Xu , Wenjing Tian . Dual-responsive nanogels with high drug loading for enhanced tumor targeting and treatment. Chinese Chemical Letters, 2025, 36(10): 110775-. doi: 10.1016/j.cclet.2024.110775
4. [4]
  Zhenyu Ji , Yucheng Xiao , Yunzhe Zhou , Ting Zhao , Lingna Yang , Cheng Chen , Mingyan Wu , Maochun Hong . A stable Zr-MOF with trap-and-flow channels for efficient natural gas purification. Chinese Journal of Structural Chemistry, 2026, 45(4): 100862-100862. doi: 10.1016/j.cjsc.2025.100862
5. [5]
  Mingxiao Liu , Wenzhuo Zheng , Jianru Yi , Jiahe Li , Jiyao Li , Yi Deng , Fusong Yuan , Kunneng Liang , Zhihe Zhao . Synergistic photocatalysis and biochemical reactivity in MXene/CuO₂ nanofibrous membrane for enhanced sterilization and tissue regeneration in chronic skin wounds. Chinese Chemical Letters, 2026, 37(9): 111985-. doi: 10.1016/j.cclet.2025.111985
6. [6]
  Chenlu Huang , Xinyu Yang , Qingyu Yu , Linhua Zhang , Dunwan Zhu . Gas-generating polymersomes-based amplified photoimmunotherapy for abscopal effect and tumor metastasis inhibition. Chinese Chemical Letters, 2024, 35(6): 109680-. doi: 10.1016/j.cclet.2024.109680
7. [7]
  Qiuxia Tan , E Pang , Qin Wang , Yuanyu Tang , Pan Zhu , Shaojing Zhao , Jianing Yi , Shiguang Jin , Minhuan Lan . Manganese carbonate-based nanoplatform for starvation therapy cascaded chemodynamic therapy, enhanced phototherapy and immune activation. Chinese Chemical Letters, 2025, 36(10): 110770-. doi: 10.1016/j.cclet.2024.110770
8. [8]
  Jianyuan Li , Jing-Hui Zhu , Yingnan Wu , Yingying Zhang , Mingrui Gu , Yahui Chen , Mingle Li , Xiaoqiang Chen , Xiaojun Peng . Tailoring NanoLuc luciferase for self-illuminated imaging, biosensing, and deep-tissue phototherapy. Chinese Chemical Letters, 2026, 37(10): 112137-. doi: 10.1016/j.cclet.2025.112137
9. [9]
  Xiangshuai Li , Jian Zhao , Li Luo , Zhuohao Jiao , Ying Shi , Shengli Hou , Bin Zhao . Visual and portable detection of metronidazole realized by metal-organic framework flexible sensor and smartphone scanning. Chinese Chemical Letters, 2024, 35(10): 109407-. doi: 10.1016/j.cclet.2023.109407
10. [10]
  Xu Huang , Kai-Yin Wu , Chao Su , Lei Yang , Bei-Bei Xiao . Metal-organic framework Cu-BTC for overall water splitting: A density functional theory study. Chinese Chemical Letters, 2025, 36(4): 109720-. doi: 10.1016/j.cclet.2024.109720
11. [11]
  Le-Tian Zhang , Bin Xia , Nan Lu , Quan-Wen Li , Xia Zhang , Na Li , Xian-He Bu . A novel naphthalenediimide-based metal-organic framework for inkless erasable printing with ultra-long cycling performance. Chinese Chemical Letters, 2025, 36(12): 110534-. doi: 10.1016/j.cclet.2024.110534
12. [12]
  Yuxin Wang , Zhengxuan Song , Yutao Liu , Yang Chen , Jinping Li , Libo Li , Jia Yao . Methyl functionalization of trimesic acid in copper-based metal-organic framework for ammonia colorimetric sensing at high relative humidity. Chinese Chemical Letters, 2024, 35(6): 108779-. doi: 10.1016/j.cclet.2023.108779
13. [13]
  Ning Zhang , Mengjie Qin , Jiawen Zhu , Xuejing Lou , Xiao Tian , Wende Ma , Youmei Wang , Minghua Lu , Zongwei Cai . Thickness-controllable synthesis of metal-organic framework based hollow nanoflowers with magnetic core via liquid phase epitaxy for phosphopeptides enrichment. Chinese Chemical Letters, 2025, 36(4): 110177-. doi: 10.1016/j.cclet.2024.110177
14. [14]
  Xian-Fa Jiang , Chongyun Shao , Zhongwen Ouyang , Zhao-Bo Hu , Zhenxing Wang , You Song . Generating electron spin qubit in metal-organic frameworks via spontaneous hydrolysis. Chinese Chemical Letters, 2024, 35(7): 109011-. doi: 10.1016/j.cclet.2023.109011
15. [15]
  Xuying Yu , Jiarong Mi , Yulan Han , Cai Sun , Mingsheng Wang , Guocong Guo . A stable radiochromic semiconductive viologen-based metal–organic framework for dual-mode direct X-ray detection. Chinese Chemical Letters, 2024, 35(9): 109233-. doi: 10.1016/j.cclet.2023.109233
16. [16]
  Jun-Yang Wang , Yu-Qing Wei , Qing-Ning Wang , Zhi-Guo Wang , Rui Hong , Lisha Yi , Ping Xu , Jia-Zhuang Xu , Zhong-Ming Li , Baisong Zhao . Mucus-inspired lubricative antibacterial coating to reduce airway complications in an intubation cynomolgus monkey model. Chinese Chemical Letters, 2025, 36(8): 110559-. doi: 10.1016/j.cclet.2024.110559
17. [17]
  Lei Li , Annan Liu , Gang Wei , Xingchen Li , Hao Liang , Maja D. Nešić , Ze Wang , Hui Guo , Quan Lin . Advanced nanosystem for target recognition and precise dual-mode imaging-guided photothermal therapy against triple-negative breast cancer. Chinese Chemical Letters, 2026, 37(2): 110961-. doi: 10.1016/j.cclet.2025.110961
18. [18]
  Jueqiong Wang , Liwei Cheng , Yang Yang , Dewen He , Yingtong Fan , Zhiwei Li , Junhao Lu , Yumin Wang , Jia Lei , Zhiyong Peng , Aiping Jin , Dan Zhou , Zhizai Li , Shuaihua Wang , Lixi Chen , Yaxing Wang , Yanlong Wang , Shuao Wang . Flexible two-dimensional uranium-organic framework with upgraded radiation resistant for X-ray imaging. Chinese Chemical Letters, 2026, 37(5): 111614-. doi: 10.1016/j.cclet.2025.111614
19. [19]
  Ran Gao , Qian Zou , Qian-Qian Su , Xiu-Fang Ma , Ye-Hui Qin , Rui Liao , Song-Song Bao , Li-Min Zheng . Photoresponsive lanthanide-dianthracene framework: Introduction of photoactive anthracene pairs by controlling the synthesis temperature. Chinese Chemical Letters, 2025, 36(10): 110404-. doi: 10.1016/j.cclet.2024.110404
20. [20]
  Guang Yang , Lu Yu , Ze-Yu Ruan , Ai-Qi Jian , Yan-Ru Chen , Yan-Cong Chen , Zhao-Ping Ni , Ming-Liang Tong . Self-assembly of spin crossover Fe(Ⅱ) framework materials with new topologies using tetra(pyridinyl)benzenediamine ligand. Chinese Chemical Letters, 2026, 37(10): 111656-. doi: 10.1016/j.cclet.2025.111656

Get Citation

PDF

XML

Metrics

PDF Downloads(7)
Abstract views(2412)
HTML views(132)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142