Citation: Gaowa Xing, Yuting Shang, Xiaorui Wang, Zengnan Wu, Qiang Zhang, Jiebing Ai, Qiaosheng Pu, Ling Lin. A microfluidic biosensor for multiplex immunoassay of foodborne pathogens agitated by programmed audio signals[J]. Chinese Chemical Letters, ;2024, 35(10): 109491. doi: 10.1016/j.cclet.2024.109491 shu

A microfluidic biosensor for multiplex immunoassay of foodborne pathogens agitated by programmed audio signals

Gaowa Xing ^{a, b, d, e, 1} ,
Yuting Shang ^{d, 1} ,
Xiaorui Wang ^c ,
Zengnan Wu ^d ,
Qiang Zhang ^d ,
Jiebing Ai ^e ,
Qiaosheng Pu ^{e, *,} ,
Ling Lin ^{c, *,}

a.
Key Laboratory of Ecology-Toxicological Effects and Control for Emerging Contaminants of Fujian Province, College of Environmental & Biological Engineering, Putian University, Putian 351100, China
b.
Xining Urban Vocational & Technical College, Xining 810000, China
c.
Department of Bioengineering, Beijing Technology and Business University, Beijing 100048, China
d.
Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China
e.
College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China

puqs@lzu.edu.cn

linling@btbu.edu.cn

Received Date: 23 August 2023
Revised Date: 7 December 2023
Accepted Date: 1 January 2024
Available Online: 6 January 2024

Figures(4)

Foods are often contaminated by multiple foodborne pathogens, which threatens human health. In this work, we developed a microfluidic biosensor for multiplex immunoassay of foodborne bacteria with agitation driven by programmed audio signals. This agitation, powered by the vibration of a speaker cone during music playing, accelerated the mass transport in the incubation process to form bacterial complexes within 10 min. Immunoassay reagents of the two target bacteria (Escherichia coli O157:H7 and Salmonella typhimurium) were preloaded into the corresponding fore-vacuum storage chamber on the chip, and released to participate in the subsequent immune analysis process by piercing the chambers. All the detection processes were integrated into a single microfluidic chip and controlled by a smartphone through Bluetooth. Under selected conditions, wide linear ranges and low limits of detection (LODs < 2 CFU/mL) were obtained, and real food samples were successfully determined within 30 min. This biosensing method can be extended to wide-ranging applications by loading different recognizing reagents.
- Multiplex immunoassay,
- Microfluidic biosensor,
- Audio signals,
- Foodborne bacteria,
- Smartphone
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