Citation: Xiaohong Wen, Mei Yang, Lie Li, Mingmin Huang, Wei Cui, Suping Li, Haiyan Chen, Chen Li, Qiuping Guo. Enzymatically controlled DNA tetrahedron nanoprobes for specific imaging of ATP in tumor[J]. Chinese Chemical Letters, ;2024, 35(8): 109291. doi: 10.1016/j.cclet.2023.109291 shu

Enzymatically controlled DNA tetrahedron nanoprobes for specific imaging of ATP in tumor

College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China

huangxiaoqin@hnu.edu.cn

guoqping@hnu.edu.cn

Received Date: 4 September 2023
Revised Date: 6 November 2023
Accepted Date: 7 November 2023
Available Online: 10 November 2023

Figures(5)

Intracellular ATP is an emerging biomarker for cancer early diagnosis because it is a key messenger for regulating the proliferation and migration of cancer cells. However, the conventional ATP biosensing strategy is often limited by the undesired on-target off-tumor interference. Here, we reported a novel strategy to design enzymatically controlled DNA tetrahedron nanoprobes (En-DT) for biosensing and imaging ATP in tumor cells. The En-DT was designed via rational engineering of structure-switching aptamers with the incorporation of an enzyme-activatable site and further conjugation on the DNA tetrahedron. The En-DT could be catalytically activated by apurinic/apyrimidinic endonuclease 1 (APE1) in cancer cells, but they did not respond to ATP in normal cells, thereby enabling cancer-specific ATP biosensing and imaging in vitro and in vivo with improved tumor specificity. This strategy would facilitate the precise detection of a broad range of biomarker in tumors and may promote the development of smart probes for cancer diagnosis.
- Enzyme-controlled,
- APE1,
- DNA tetrahedron,
- Cancer-specific,
- ATP biosensing
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