Citation: Xiaohong Wen, Mei Yang, Lie Li, Mingmin Huang, Wei Cui, Suping Li, Haiyan Chen, Chen Li, Qiuping Guo. Enzymatically controlled DNA tetrahedron nanoprobes for specific imaging of ATP in tumor[J]. Chinese Chemical Letters, ;2024, 35(8): 109291. doi: 10.1016/j.cclet.2023.109291 shu

Enzymatically controlled DNA tetrahedron nanoprobes for specific imaging of ATP in tumor

College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China

huangxiaoqin@hnu.edu.cn

guoqping@hnu.edu.cn

Received Date: 4 September 2023
Revised Date: 6 November 2023
Accepted Date: 7 November 2023
Available Online: 10 November 2023

Figures(5)

Intracellular ATP is an emerging biomarker for cancer early diagnosis because it is a key messenger for regulating the proliferation and migration of cancer cells. However, the conventional ATP biosensing strategy is often limited by the undesired on-target off-tumor interference. Here, we reported a novel strategy to design enzymatically controlled DNA tetrahedron nanoprobes (En-DT) for biosensing and imaging ATP in tumor cells. The En-DT was designed via rational engineering of structure-switching aptamers with the incorporation of an enzyme-activatable site and further conjugation on the DNA tetrahedron. The En-DT could be catalytically activated by apurinic/apyrimidinic endonuclease 1 (APE1) in cancer cells, but they did not respond to ATP in normal cells, thereby enabling cancer-specific ATP biosensing and imaging in vitro and in vivo with improved tumor specificity. This strategy would facilitate the precise detection of a broad range of biomarker in tumors and may promote the development of smart probes for cancer diagnosis.
- Enzyme-controlled,
- APE1,
- DNA tetrahedron,
- Cancer-specific,
- ATP biosensing
1. [1]
  M.T. Lin, M.F. Beal, Nature 443 (2006) 787–795. doi: 10.1038/nature05292
2. [2]
  A. Patel, L. Malinovska, S. Saha, Science 365 (2017) 753–756. doi: 10.1126/science.aaf6846
3. [3]
  P.B. Dennis, A. Jaeschke, M. Saitoh, et al., Science 294 (2001) 1102–1105. doi: 10.1126/science.1063518
4. [4]
  J.R. Knowles, Annu. Rev. Biochem. 49 (1980) 877–919. doi: 10.1146/annurev.bi.49.070180.004305
5. [5]
  J.H. Young, J. Mclick, E.F. Korman, Nature 249 (1974) 474–476. doi: 10.1038/249474a0
6. [6]
  M.I. Sweeney, Neurosci. Biobehav. Rev. 21 (1997) 207–217. doi: 10.1016/S0149-7634(96)00011-5
7. [7]
  T. Foltynie, Lancet Neurol. 18 (2019) 1072–1074. doi: 10.1016/S1474-4422(19)30404-1
8. [8]
  D.C. Wallace, Science 283 (1999) 1482–1488. doi: 10.1126/science.283.5407.1482
9. [9]
  E. Adinolfi, L. Raffaghello, A.L. Giuliani, et al., Cancer Res. 72 (2012) 2957–2969.
10. [10]
  S.M. Gilbert, C.J. Oliphant, S. Hassan, Oncogene 38 (2018) 194–208.
11. [11]
  D.E. Huizenga, J.W. Szostak, Biochemistry 34 (1995) 656–665. doi: 10.1021/bi00002a033
12. [12]
  D. Zheng, D.S. Seferos, D.A. Giljohann, et al., Nano Lett. 9 (2009) 3258–3261. doi: 10.1021/nl901517b
13. [13]
  Y. Xiang, Y. Lu, Nat. Chem. 3 (2011) 697–703. doi: 10.1038/nchem.1092
14. [14]
  Z. Liu, S. Chen, B. Liu, et al., Anal. Chem. 86 (2014) 12229–12235. doi: 10.1021/ac503358m
15. [15]
  H.K. Walter, J. Bauer, J. Steinmeyer, et al., Nano Lett. 17 (2017) 2467–2472. doi: 10.1021/acs.nanolett.7b00159
16. [16]
  J. Zhao, J. Gao, W. Xue, et al., J. Am. Chem. Soc. 140 (2018) 578–581. doi: 10.1021/jacs.7b11161
17. [17]
  X. Meng, H. Wang, M. Yang, et al., Anal. Chem. 93 (2021) 1693–1701. doi: 10.1021/acs.analchem.0c04302
18. [18]
  Z. Xiang, J. Zhao, D. Yi, et al., Chem. Int. Ed. 60 (2021) 22659–22663. doi: 10.1002/anie.202106639
19. [19]
  D. Zhao, D. Chang, Q. Zhang, et al., J. Am. Chem. Soc. 143 (2021) 15084–15090. doi: 10.1021/jacs.1c04925
20. [20]
  A.E. Rangel, A.A. Hariri, M. Eisenstein, et al., Adv. Mater. 32 (2020) 2003704. doi: 10.1002/adma.202003704
21. [21]
  I.V. Nesterova, E.E. Nesterov, J. Am. Chem. Soc. 136 (2014) 8843–8846. doi: 10.1021/ja501859w
22. [22]
  F. Zhou, T. Fu, Q. Huang, et al., J. Am. Chem. Soc. 141 (2019) 18421–18427. doi: 10.1021/jacs.9b05063
23. [23]
  N. Xie, J. Huang, X. Yang, et al., ACS Sensors 1 (2016) 1445–1452. doi: 10.1021/acssensors.6b00593
24. [24]
  Z. Ge, H. Gu, Q. Li, et al., J. Am. Chem. Soc. 140 (2018) 17808–17819. doi: 10.1021/jacs.8b10529
25. [25]
  L. He, D. Lu, H. Liang, et al., J. Am. Chem. Soc. 140 (2018) 258–263. doi: 10.1021/jacs.7b09789
26. [26]
  Z. Liu, H. Pei, L. Zhang, et al., ACS Nano 12 (2018) 12357–12368. doi: 10.1021/acsnano.8b06322
27. [27]
  K. Zhang, W. Huang, Y. Huang, et al., Anal. Chem. 91 (2019) 7086–7096. doi: 10.1021/acs.analchem.9b00011
28. [28]
  Y. Zhang, Y. Deng, C. Wang, et al., Chem. Sci. 10 (2019) 5959–5966. doi: 10.1039/c9sc01912j
29. [29]
  W. Fan, B. Yung, P. Huang, et al., Chem. Rev. 117 (2017) 13566–13638. doi: 10.1021/acs.chemrev.7b00258
30. [30]
  S.G. Harroun, C. Prevost-Tremblay, D. Lauzon, et al., Nanoscale 10 (2018) 4607–4641. doi: 10.1039/c7nr07348h
31. [31]
  L. Li, J. Wang, H.S. Jiang, et al., Chin. Chem. Lett. 34 (2023) 107506. doi: 10.1016/j.cclet.2022.05.020
32. [32]
  D. Yi, J. Zhao, L. Li, Angew. Chem. Int. Ed. 60 (2021) 6300–6304. doi: 10.1002/anie.202015979
33. [33]
  C.D. Mol, T. Izumi, S. Mitra, et al., Nature 403 (2000) 451–456. doi: 10.1038/35000249
34. [34]
  F. Shah, D. Logsdon, R.A. Messmann, et al., NPJ Precis. Oncol. 1 (2017) 19. doi: 10.1038/s41698-017-0023-0
35. [35]
  S. Choi, H.K. Joo, B.H. Jeon, Chonnam Med. J. 52 (2016) 75–80. doi: 10.4068/cmj.2016.52.2.75
36. [36]
  J. Verrax, N. Dejeans, B. Sid, et al., Biochem. Pharmacol. 82 (2011) 1540–1548. doi: 10.1016/j.bcp.2011.07.102
37. [37]
  M.L. Fishel, M.R. Kelley, Mol. Aspects Med. 28 (2007) 375–395. doi: 10.1016/j.mam.2007.04.005
38. [38]
  R.L. Juliano, Nucl. Acids Res. 44 (2016) 6518–6548. doi: 10.1093/nar/gkw236
39. [39]
  R. Yan, J. Chen, J. Wang, et al., Small 14 (2018) 1802745. doi: 10.1002/smll.201802745
40. [40]
  X. Zhu, B. Qu, Z.M. Ying, et al., Anal. Chem. 92 (2020) 15953–15958. doi: 10.1021/acs.analchem.0c03400
1. [1]
  Zhen Dai , Linzhi Tan , Yeyu Su , Kerui Zhao , Yushun Tian , Yu Liu , Tao Liu . Site-specific incorporation of reduction-controlled guest amino acids into proteins for cucurbituril recognition. Chinese Chemical Letters, 2024, 35(5): 109121-. doi: 10.1016/j.cclet.2023.109121
2. [2]
  Jun Xiong , Ke-Ke Chen , Neng-Bin Xie , Wei Chen , Wen-Xuan Shao , Tong-Tong Ji , Si-Yu Yu , Yu-Qi Feng , Bi-Feng Yuan . Demethylase-assisted site-specific detection of N¹-methyladenosine in RNA. Chinese Chemical Letters, 2024, 35(5): 108953-. doi: 10.1016/j.cclet.2023.108953
3. [3]
  Lixian Fu , Yiyun Tan , Yue Ding , Weixia Qing , Yong Wang . Water–soluble and polarity–sensitive near–infrared fluorescent probe for long–time specific cancer cell membranes imaging and C. Elegans label. Chinese Chemical Letters, 2024, 35(4): 108886-. doi: 10.1016/j.cclet.2023.108886
4. [4]
  Chang Liu , Tao Wu , Lijiao Deng , Xuzi Li , Xin Fu , Shuzhen Liao , Wenjie Ma , Guoqiang Zou , Hai Yang . Programmed DNA walkers for biosensors. Chinese Chemical Letters, 2024, 35(9): 109307-. doi: 10.1016/j.cclet.2023.109307
5. [5]
  Erzhuo Cheng , Yunyi Li , Wei Yuan , Wei Gong , Yanjun Cai , Yuan Gu , Yong Jiang , Yu Chen , Jingxi Zhang , Guangquan Mo , Bin Yang . Galvanostatic method assembled ZIFs nanostructure as novel nanozyme for the glucose oxidation and biosensing. Chinese Chemical Letters, 2024, 35(9): 109386-. doi: 10.1016/j.cclet.2023.109386
6. [6]
  Jia-Li Xie , Tian-Jin Xie , Yu-Jie Luo , Kai Mao , Cheng-Zhi Huang , Yuan-Fang Li , Shu-Jun Zhen . Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection. Chinese Chemical Letters, 2024, 35(6): 109137-. doi: 10.1016/j.cclet.2023.109137
7. [7]
  Jia-Mei Qin , Xue Li , Wei Lang , Fu-Hao Zhang , Qian-Yong Cao . An AIEgen nano-assembly for simultaneous detection of ATP and H₂S. Chinese Chemical Letters, 2024, 35(6): 108925-. doi: 10.1016/j.cclet.2023.108925
8. [8]
  Yang Qin , Jiangtian Li , Xuehao Zhang , Kaixuan Wan , Heao Zhang , Feiyang Huang , Limei Wang , Hongxun Wang , Longjie Li , Xianjin Xiao . Toeless and reversible DNA strand displacement based on Hoogsteen-bond triplex. Chinese Chemical Letters, 2024, 35(5): 108826-. doi: 10.1016/j.cclet.2023.108826
9. [9]
  Jingwen Zhao , Jianpu Tang , Zhen Cui , Limin Liu , Dayong Yang , Chi Yao . A DNA micro-complex containing polyaptamer for exosome separation and wound healing. Chinese Chemical Letters, 2024, 35(9): 109303-. doi: 10.1016/j.cclet.2023.109303
10. [10]
  Fei Yin , Erli Yang , Xue Ge , Qian Sun , Fan Mo , Guoqiu Wu , Yanfei Shen . Coupling WO₃₋_x dots-encapsulated metal-organic frameworks and template-free branched polymerization for dual signal-amplified electrochemiluminescence biosensing. Chinese Chemical Letters, 2024, 35(4): 108753-. doi: 10.1016/j.cclet.2023.108753
11. [11]
  Tian Feng , Yun-Ling Gao , Di Hu , Ke-Yu Yuan , Shu-Yi Gu , Yao-Hua Gu , Si-Yu Yu , Jun Xiong , Yu-Qi Feng , Jie Wang , Bi-Feng Yuan . Chronic sleep deprivation induces alterations in DNA and RNA modifications by liquid chromatography-mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(8): 109259-. doi: 10.1016/j.cclet.2023.109259
12. [12]
  Zhe-Han Yang , Jie Yin , Lei Xin , Yuanfang Li , Yijie Huang , Ruo Yuan , Ying Zhuo . Research advancement of DNA-based intelligent hydrogels: Manufacture, characteristics, application of disease diagnosis and treatment. Chinese Chemical Letters, 2024, 35(10): 109558-. doi: 10.1016/j.cclet.2024.109558
13. [13]
  Huan Yao , Jian Qin , Yan-Fang Wang , Song-Meng Wang , Liu-Huan Yi , Shi-Yao Li , Fangfang Du , Liu-Pan Yang , Li-Li Wang . Ultra-highly selective recognition of nucleosides over nucleotides by rational modification of tetralactam macrocycle and its application in enzyme assay. Chinese Chemical Letters, 2024, 35(6): 109154-. doi: 10.1016/j.cclet.2023.109154
14. [14]
  Yunan Yuan , Zhimin Luo , Jie Chen , Chaoliang He , Kai Hao , Huayu Tian . Constructing thermoresponsive PNIPAM-based microcarriers for cell culture and enzyme-free cell harvesting. Chinese Chemical Letters, 2024, 35(7): 109549-. doi: 10.1016/j.cclet.2024.109549
15. [15]
  Junjie Wang , Yan Wang , Zhengdong Li , Changqiang Xie , Musammir Khan , Xingzhou Peng , Fabiao Yu . Triphenylamine-AIEgens photoactive materials for cancer theranostics. Chinese Chemical Letters, 2024, 35(6): 108934-. doi: 10.1016/j.cclet.2023.108934
16. [16]
  Ming Huang , Xiuju Cai , Yan Liu , Zhuofeng Ke . Base-controlled NHC-Ru-catalyzed transfer hydrogenation and α-methylation/transfer hydrogenation of ketones using methanol. Chinese Chemical Letters, 2024, 35(7): 109323-. doi: 10.1016/j.cclet.2023.109323
17. [17]
  Hao Cai , Xiaoyan Wu , Lei Jiang , Feng Yu , Yuxiang Yang , Yan Li , Xian Zhang , Jian Liu , Zijian Li , Hong Bi . Lysosome-targeted carbon dots with a light-controlled nitric oxide releasing property for enhanced photodynamic therapy. Chinese Chemical Letters, 2024, 35(4): 108946-. doi: 10.1016/j.cclet.2023.108946
18. [18]
  Wenyu Gao , Liming Zhang , Chuang Zhao , Lixiang Liu , Xingran Yang , Jinbo Zhao . Controlled semi-Pinacol rearrangement on a strained ring: Efficient access to multi-substituted cyclopropanes by group migration strategy. Chinese Chemical Letters, 2024, 35(9): 109447-. doi: 10.1016/j.cclet.2023.109447
19. [19]
  Jianhui Yin , Wenjing Huang , Changyong Guo , Chao Liu , Fei Gao , Honggang Hu . Tryptophan-specific peptide modification through metal-free photoinduced N-H alkylation employing N-aryl glycines. Chinese Chemical Letters, 2024, 35(6): 109244-. doi: 10.1016/j.cclet.2023.109244
20. [20]
  Ying Zhao , Yin-Hang Chai , Tian Chen , Jie Zheng , Ting-Ting Li , Francisco Aznarez , Li-Long Dang , Lu-Fang Ma . Size-controlled synthesis and near-infrared photothermal response of Cp* Rh-based metalla[2]catenanes and rectangular metallamacrocycles. Chinese Chemical Letters, 2024, 35(6): 109298-. doi: 10.1016/j.cclet.2023.109298

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(293)
HTML views(1)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142