Citation: Yuxin Tian, Mengjun Li, Yang Yang, Chunhui Li, Yun Peng, Haiyin Yang, Mengyuan Zhao, Pengfei Wu, Shaobo Ruan, Yuanyu Huang, Chenguang Shen, Minghui Yang. An MPXV mRNA-LNP vaccine candidate elicits protective immune responses against monkeypox virus[J]. Chinese Chemical Letters, ;2024, 35(8): 109270. doi: 10.1016/j.cclet.2023.109270 shu

An MPXV mRNA-LNP vaccine candidate elicits protective immune responses against monkeypox virus

Yuxin Tian ^{a, 1} ,
Mengjun Li ^{b, 1} ,
Yang Yang ^{c, 1} ,
Chunhui Li ^a ,
Yun Peng ^c ,
Haiyin Yang ^a ,
Mengyuan Zhao ^a ,
Pengfei Wu ^a ,
Shaobo Ruan ^a ,
Yuanyu Huang ^{a, *,} ,
Chenguang Shen ^{b, *,} ,
Minghui Yang ^{a, *,}

a.
Advanced Research Institute of Multidisciplinary Science, School of Life Science, Key Laboratory of Molecular Medicine and Biotherapy, Beijing Institute of Technology, Beijing 100081, China
b.
BSL-3 Laboratory (Guangdong), Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China
c.
National Clinical Research Center for infectious disease, Shenzhen Third People's Hospital, Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen 518112, China

yyhuang@bit.edu.cn

a124965468@smu.edu.cn

yangmh17@bit.edu.cn

Received Date: 30 August 2023
Revised Date: 25 October 2023
Accepted Date: 29 October 2023
Available Online: 4 November 2023

Figures(4)

The monkeypox virus (MPXV) outbreak, declared a Public Health Emergency of International Concern (PHEIC) by the World Health Organization (WHO) in 2022, continues to pose a significant threat due to the absence of vaccines or drugs for MPXV infection. In this study, we developed an mRNA vaccine that expressing the A29L antigen, a specific protein of the intracellular mature virus. Our vaccine utilizes a thermostable ionizable lipid nanoparticle (iLNP) platform and has been administered to mice. Our findings demonstrate that the MPXV A29L mRNA vaccine candidate induces robust cross-neutralizing immune responses against both vaccinia virus (VACV) and MPXV live virus. Furthermore, immunization with the vaccine candidate provided protection against the VACV challenge in mice. These findings underscore the potential of mRNA-LNP vaccines as safe and effective candidates against monkeypox epidemics. Given the current absence of specific interventions for MPXV infection, our study represents a significant step forward in developing a viable solution to combat this ongoing public health threat.
- Monkeypox virus,
- mRNA vaccine,
- Lipid-nano particle,
- A29L,
- VACV
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