Citation: Bin Fang, Jiaqi Yang, Limin Wang, Haoqin Li, Jiaying Guo, Jiaxin Zhang, Qingyuan Guo, Bo Peng, Kedi Liu, Miaomiao Xi, Hua Bai, Li Fu, Lin Li. A mitochondria-targeted H₂S-activatable fluorogenic probe for tracking hepatic ischemia-reperfusion injury[J]. Chinese Chemical Letters, ;2024, 35(6): 108913. doi: 10.1016/j.cclet.2023.108913 shu

A mitochondria-targeted H₂S-activatable fluorogenic probe for tracking hepatic ischemia-reperfusion injury

Bin Fang ^{a, b, 1} ,
Jiaqi Yang ^{b, 1} ,
Limin Wang ^{b, 1} ,
Haoqin Li ^b ,
Jiaying Guo ^b ,
Jiaxin Zhang ^b ,
Qingyuan Guo ^b ,
Bo Peng ^{b, c, e} ,
Kedi Liu ^f ,
Miaomiao Xi ^f ,
Hua Bai ^{b, c, *,} ,
Li Fu ^{a, *,} ,
Lin Li ^{b, d, e, *,}

a.
School of Materials Science and Engineering, Northwestern Polytechnical University, Xi'an 710072, China
b.
Frontiers Science Center for Flexible Electronics, Xi'an Institute of Flexible Electronics (IFE) and Xi'an Institute of Biomedical Materials & Engineering, Northwestern Polytechnical University, Xi'an 710072, China
c.
Key laboratory of Flexible Electronics of Zhejiang Province, Ningbo Institute of Northwestern Polytechnical University, Ningbo 315103, China
d.
The Institute of Flexible Electronics (IFE, Future Technologies), Xiamen University, Xiamen 361005, China
e.
Wuhan National Laboratory for Optoelectronics - Advanced Biomedical Imaging Facility, Huazhong University of Science and Technology, Wuhan 430074, China
f.
TANK Medicinal Biology Institute of Xi'an, Xi'an 710032, China

iamhbai@nwpu.edu.cn

fuli@nwpu.edu.cn

ifelli@xmu.edu.cn

Received Date: 25 June 2023
Revised Date: 5 August 2023
Accepted Date: 7 August 2023
Available Online: 9 August 2023

Figures(6)

Hepatic ischemia-reperfusion injury (HIRI) is the cause of postoperative hepatic dysfunction and failure, and even death. As an important biological effector molecule, hydrogen sulfide (H₂S) of mitochondria as a gasotransmitter that is usually used to protect against acute HIRI injury. However, the exact relationship between HIRI and mitochondrial H₂S remains tangled due to the lack of an effective analytical method. Herein, we have fabricated a mitochondria-targeted H₂S-activatable fluorogenic probe (Mito-GW) to explore the stability of mitochondrial H₂S and track the changes of mitochondrial H₂S during the HIRI. By virtue of pyridinium electropositivity and its amphiphilicity, Mito-GW could accumulate in mitochondria. It goes through an analyte-prompted immolation when reacts with H₂S, resulting in the releasing of the fluorophore (GW). Therefore, the extent of Mito-GW conversion to GW can be used to evaluate the changes of mitochondrial H₂S level in living cells and tissues. As proof-of-principle, we have used Mito-GW to demonstrate the mitochondria H₂S-levels increase and then decrease during HIRI in vitro and in vivo. Our research highlights the tremendous potential of Mito-GW as a mitochondrial H₂S fluorogenic probe in elucidating the pathogenesis of HIRI, providing a powerful tool for promoting future research on hepatology.
- Mitochondria-targeted,
- Bioimaging,
- H₂S-activatable,
- Fluorogenic probe,
- Hepatic ischemia-reperfusion injury (HIRI)
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通讯作者: 陈斌, bchen63@163.com

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A mitochondria-targeted H2S-activatable fluorogenic probe for tracking hepatic ischemia-reperfusion injury

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通讯作者: 陈斌, bchen63@163.com

A mitochondria-targeted H₂S-activatable fluorogenic probe for tracking hepatic ischemia-reperfusion injury