Citation: Ding Wei, Yurong Mao, Huihui Wang, Siqi Qu, Jiakang Chen, Jiusheng Li, Biao Jiang, Hongli Chen. A mild phenoxysilyl linker for self-immolative release of antibody-drug conjugates[J]. Chinese Chemical Letters, ;2023, 34(5): 108091. doi: 10.1016/j.cclet.2022.108091 shu

A mild phenoxysilyl linker for self-immolative release of antibody-drug conjugates

Ding Wei ^{a, b, c, 1} ,
Yurong Mao ^{b, c, 1} ,
Huihui Wang ^{b, c} ,
Siqi Qu ^{b, c} ,
Jiakang Chen ^b ,
Jiusheng Li ^{a, *,} ,
Biao Jiang ^{b, *,} ,
Hongli Chen ^{b, *,}

a.
Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China
b.
Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai 201210, China
c.
University of Chinese Academy of Sciences, Beijing 100049, China

lijs@sari.ac.cn

jiangbiao@shanghaitech.edu.cn

chenhl@shanghaitech.edu.cn

Received Date: 23 July 2022
Revised Date: 16 December 2022
Accepted Date: 21 December 2022
Available Online: 23 December 2022

Figures(4)

Self-immolative linkers have been widely used to construct prodrugs to improve their efficacy and safety. In this study, we report the use of phenoxysilyl linker as a self-immolative unit to prepare antibody-drug conjugates (ADCs). Phenoxysily based ADC Ate-PPS-CA4 was prepared and its release was systematically investigated by mass spectrometry. Biological evaluation showed that Ate-PPS-CA4 displayed the ability to target delivery and self-immolative release the active payload CA4 on PD-L1 positive cells MDA-MB-231. As the same with its payload CA4, it could arrest the cell cycle to the G2/M phase and induced changes in cell morphology at the dose of its IC₅₀. The development of this linker with novel drug release mechanisms will expand the methodology to construct ADCs, especially for non-internalizing ADCs by extracellular cleavage.
- Antibody-drug conjugates,
- Self-immolative release,
- Phenoxysilyl linker,
- Combretastatin A4 (CA4)
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