Citation: Zhaoyan Tian, Xiang Qin, Fengying Shao, Xiuxiu Li, Zhi Wang, Songqin Liu, Yafeng Wu. Electrofluorochromic imaging analysis of dopamine release from living PC12 cells with bipolar nanoelectrodes array[J]. Chinese Chemical Letters, ;2023, 34(1): 107656. doi: 10.1016/j.cclet.2022.06.079 shu

Electrofluorochromic imaging analysis of dopamine release from living PC12 cells with bipolar nanoelectrodes array

Zhaoyan Tian ^a ,
Xiang Qin ^b ,
Fengying Shao ^a ,
Xiuxiu Li ^a ,
Zhi Wang ^c ,
Songqin Liu ^{a, *,} ,
Yafeng Wu ^{a, *,}

a.
Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, Jiangsu Province Hi-Tech Key Laboratory for Bio-Medical Research, State Key Laboratory of Bioelectronics, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China
b.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China
c.
Wuxi Institute of Inspection, Testing and Certification, Wuxi 214125, China

liusq@seu.edu.cn

wuyafeng@seu.edu.cn

Received Date: 6 June 2022
Revised Date: 15 June 2022
Accepted Date: 30 June 2022
Available Online: 1 July 2022

Figures(6)

The coupling of bipolar electrode (BPE) arrays and electrofluorochromic (EFC) imaging has exhibited great abilities in bioanalysis. However, the imaging resolution and analytical performance are hampered by the large size of the electrode and the rapid diffusion of EFC molecules on the electrode surface. Here, to address the challenges, bipolar nanoelectrodes (BPnE) array and in situ immobilization strategy of EFC molecules were proposed. Anodized aluminum oxide (AAO) template-assisted Au nanoelectrodes array with high density was fabricated as BPnE array for high spatial imaging resolution. By electrically polymerizing EFC molecules on the surface of single Au nanoelectrode, the rapid diffusion of EFC molecules on the electrode surface was not only avoided, but also realizing electrofluorescent imaging on an individual nanoelectrode. Using dopamine (DA) released from living PC12 cells as a model, the proposed strategy exhibited an ultra-high sensitivity for DA analysis with a detection limit of 0.45 nmol/L and the DA release amount from a single cell was calculated to be 0.13 pmol/L. Moreover, the dynamic change of DA release under the drug stimulation from living PC12 cells could also be monitored.
- Au nanoelectrodes array,
- Bipolar nanoelectrodes,
- Dopamine release,
- Electrofluorochromic imaging,
- Real-time monitoring
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