Citation: Huili Wang, Yishuo Sun, Xuemei Lin, Wei Feng, Zhanxian Li, Mingming Yu. Multi-organelle-targeting pH-dependent NIR fluorescent probe for lysosomal viscosity[J]. Chinese Chemical Letters, ;2023, 34(3): 107626. doi: 10.1016/j.cclet.2022.06.049 shu

Multi-organelle-targeting pH-dependent NIR fluorescent probe for lysosomal viscosity

Huili Wang ^{a, 1} ,
Yishuo Sun ^{b, 1} ,
Xuemei Lin ^a ,
Wei Feng ^b ,
Zhanxian Li ^{a, *,} ,
Mingming Yu ^{a, *,}

a.
Green Catalysis Center and College of Chemistry, Zhengzhou University, Zhengzhou 450001, China
b.
Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, Institutes of Biomedical Sciences & Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, Shanghai 200433, China

lizx@zzu.edu.cn

yumm@zzu.edu.cn

Received Date: 30 March 2022
Revised Date: 17 June 2022
Accepted Date: 20 June 2022
Available Online: 23 June 2022

Figures(7)

The normal operation of lysosome, mitochondria, Golgi apparatus and endoplasmic reticulum plays a significant role in maintaining cell homeostasis. Reflecting the state and function of lysosomes, viscosity is a pivotal parameter to assess the stability of microenvironment. Herein, based on TICT mechanism, a new NIR pH-dependent fluorescent probe DCIC with push-pull electronic moiety was synthesized to identify the lysosomes viscosity. In viscous media, DCIC was highly sensitive to viscosity, fluorescence intensity increased by 180 times as viscosity increased from 1.0 cp to 438.4 cp. In addition, DCIC have high localization ability for lysosome, mitochondria, Golgi apparatus, and endoplasmic reticulum and can monitor lysosomal viscosity fluctuations with laser confocal microscopy.
- NIR,
- Lysosomal viscosity,
- PH-dependent,
- Multi-organelle localization
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