Citation: Lijuan Gui, Kaizhen Wang, Yuxin Wang, Jun Yan, Xian Liu, Jingxuan Guo, Ji Liu, Dawei Deng, Haiyan Chen, Zhenwei Yuan. Monitoring the pH fluctuation of lysosome under cell stress using a near-infrared ratiometric fluorescent probe[J]. Chinese Chemical Letters, ;2023, 34(3): 107586. doi: 10.1016/j.cclet.2022.06.009 shu

Monitoring the pH fluctuation of lysosome under cell stress using a near-infrared ratiometric fluorescent probe

Lijuan Gui ^{a, 1} ,
Kaizhen Wang ^{a, 1} ,
Yuxin Wang ^a ,
Jun Yan ^a ,
Xian Liu ^b ,
Jingxuan Guo ^a ,
Ji Liu ^a ,
Dawei Deng ^{a, *,} ,
Haiyan Chen ^{a, *,} ,
Zhenwei Yuan ^{a, *,}

a.
Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 210009, China
b.
Department of Health Technology and Informatics, Faculty of Health and Social Sciences, The Hong Kong Polytechnic University, Hong Kong, China

dengdawei@cpu.edu.cn

chenhaiyan@cpu.edu.cn

yuanzhenwei@cpu.edu.cn

Received Date: 14 March 2022
Revised Date: 1 June 2022
Accepted Date: 5 June 2022
Available Online: 10 June 2022

Figures(6)

Cell stress responses are associated with numerous diseases including diabetes, neurodegenerative diseases, and cancer. Several events occur under cell stress, in which, are protein expression and organelle-specific pH fluctuation. To understand the lysosomal pH variation under cell stress, a novel NIR ratiometric pH-responsive fluorescent probe (BLT) with lysosomes localization capability was developed. The quinoline ring of BLT combined with hydrogen ion which triggered the rearrangement of π electrons conjugated at low pH medium, meanwhile, the absorption and fluorescent spectra of BLT showed a red-shifts, which gived a ratiometric signal. Moreover, the probe BLT with a suitable pK_a value has the potential to discern changes in lysosomal pH, either induced by heat stress or oxidative stress or acetaminophen-induced (APAP) injury stress. Importantly, this ratiometric fluorescent probe innovatively tracks pH changes in lysosome in APAP-induced liver injury in live cells, mice, and zebrafish. The probe BLT as a novel fluorescent probe possesses important value for exploring lysosomal-associated physiological varieties of drug-induced hepatotoxicity.
- APAP-induced liver injury,
- Ratiometric fluorescent probe,
- Lysosomal pH,
- Cell stress
1. [1]
  S. Fulda, A.M. Gorman, O. Hori, A. Samali, Int. J. Cell. Biol.(2010) 214074. doi: 10.1155/2010/214074
2. [2]
  Y. Ishida, S. Nayak, J.A. Mindell, et al., J. Gen. Physiol. 141 (2013) 705-720. doi: 10.1085/jgp.201210930
3. [3]
  G.H. Sun-Wada, Y. Wada, M. Futai, Biochim. Biophys. Acta 1658 (2004) 106-114. doi: 10.1016/j.bbabio.2004.04.013
4. [4]
  J.R. Casey, S. Grinstein, J. Orlowski, Mol. Cell Biol. 11 (2010) 50-61. doi: 10.1038/nrm2820
5. [5]
  X. Liu, Y. Su, H. Tian, et al., Anal. Chem. 89 (2017) 7038-7045. doi: 10.1021/acs.analchem.7b00754
6. [6]
  X. Shi, N. Yan, G. Niu, et al., Chem. Sci. 11 (2020) 3152-3163. doi: 10.1039/c9sc06226b
7. [7]
  R.E. Lawrence, R. Zoncu, Nat. Cell Biol. 21 (2019) 133-142. doi: 10.1038/s41556-018-0244-7
8. [8]
  S.M. Davidson, M.G. Vander Heiden, Annu. Rev. Pharmacool. Toxicol. 57 (2017) 481-507. doi: 10.1146/annurev-pharmtox-010715-103101
9. [9]
  H. Hou, Y. Zhao, C. Li, et al., Sci. Rep. 7 (2017) 1759. doi: 10.1038/s41598-017-01956-1
10. [10]
  B.A. Webb, M. Chimenti, M.P. Jacobson, et al., Nat. Rev. Cancer 11 (2011) 671-677. doi: 10.1038/nrc3110
11. [11]
  G. Kroemer, M. Jäättelä, Nat. Rev. Cancer 5 (2005) 886-897. doi: 10.1038/nrc1738
12. [12]
  J. Han, K. Burgess, Chem. Rev. 110 (2010) 2709-2728. doi: 10.1021/cr900249z
13. [13]
  Y. Sun, X. Zhou, L. Sun, et al., Chin. Chem. Lett. 33 (2022) 4229–4232. doi: 10.1016/j.cclet.2022.01.087
14. [14]
  Z. Yuan, J. Chen, Q. Zhou, et al., Chin. Chem. Lett. 32 (2021) 1803-1808. doi: 10.1016/j.cclet.2020.12.012
15. [15]
  H. Ogasawara, Y. Tanaka, M. Taki, S. Yamaguchi, Chem. Sci. 12 (2021) 7902-7907. doi: 10.1039/d1sc01705e
16. [16]
  E.A. Owens, M. Henary, G. El Fakhri, et al., Acc. Chem. Res. 49 (2016) 1731-1740. doi: 10.1021/acs.accounts.6b00239
17. [17]
  C. Staudinger, S.M. Borisov, Methods Appl. Fluoresc. 3 (2015) 042005. doi: 10.1088/2050-6120/3/4/042005
18. [18]
  G. Niu, P. Zhang, W. Liu, et al., Anal. Chem. 89 (2017) 1922-1929. doi: 10.1021/acs.analchem.6b04417
19. [19]
  S.I. Reja, M. Minoshima, Y. Hori, et al., Chem. Sci. 12 (2020) 3437-3447. doi: 10.1039/d0sc04792a
20. [20]
  L. Yuan, W. Lin, S. Zhao, et al., J. Am. Chem. Soc. 134 (2012) 13510-13523. doi: 10.1021/ja305802v
21. [21]
  Q. Xia, S. Feng, J. Hong, et al., Talanta 228 (2021) 122184. doi: 10.1016/j.talanta.2021.122184
22. [22]
  T. Zhang, F. Huo, W. Zhang, et al., Sens. Actuators B: Chem. 345 (2021) 130393. doi: 10.1016/j.snb.2021.130393
23. [23]
  P. Ning, L. Hou, Y. Feng, et al., Chem. Commun. 55 (2019) 1782-1785. doi: 10.1039/c8cc09517e
24. [24]
  J. Ge, L. Fan, K. Zhang, et al., Sens. Actuat. B: Chem. 262 (2018) 913-921. doi: 10.1016/j.snb.2018.02.082
25. [25]
  G. Song, D. Jiang, L. Wang, et al., Chin. Chem. Lett. 33 (2022) 339-343. doi: 10.1016/j.cclet.2021.06.076
26. [26]
  Q. Wan, S. Chen, W. Shi, et al., Angew. Chem. Int. Ed. 53 (2014) 10916-10920. doi: 10.1002/anie.201405742
27. [27]
  H. Zhu, J. Fan, Q. Xu, et al., Chem. Commun. 48 (2012) 11766-11768. doi: 10.1039/c2cc36785h
28. [28]
  X. Chen, Q. Chen, M. Chen, et al., Sens. Actuators B: Chem. 329 (2021) 129104. doi: 10.1016/j.snb.2020.129104
29. [29]
  G. -J. Mao, Z. -Z. Liang, G. -Q. Gao, et al., Anal. Chim. Acta 1092 (2019) 117-125. doi: 10.1016/j.aca.2019.09.053
30. [30]
  D. Cheng, J. Peng, Y. Lv, et al., J. Am. Chem. Soc. 141 (2019) 6352-63 doi: 10.1021/jacs.9b01374
31. [31]
  D. Cheng, W. Xu, L. Yuan, et al., Anal. Chem. 89 (2017) 7693-7700. doi: 10.1021/acs.analchem.7b01671
32. [32]
  Y. Li, X. Xie, X. e. Yang, et al., Chem. Sci. 8 (2017) 4006-4011. doi: 10.1039/C7SC00303J
33. [33]
  Y. Liu, J. Zhou, L. Wang, et al., J. Am. Chem. Soc. 138 (2016) 12368-12374. doi: 10.1021/jacs.6b04048
34. [34]
  Y. Yue, F. Huo, X. Li, et al., Org. Lett. 19 (2017) 82-85. doi: 10.1021/acs.orglett.6b03357
35. [35]
  M.H. Lee, J.H. Han, J.H. Lee, et al., Angew. Chem. Int. Ed. 52 (2013) 6206-6209. doi: 10.1002/anie.201301894
1. [1]
  Zhoupeng Zheng , Shengyi Gong , Qianhua Li , Shiya Zhang , Guoqiang Feng . Lipid droplets and gallbladder targeted fluorescence probe for ratiometric NO imaging in gallstones disease models. Chinese Chemical Letters, 2025, 36(5): 110191-. doi: 10.1016/j.cclet.2024.110191
2. [2]
  Wei Zhang , Lei Yang , Zhihang Jin , Shusheng Zhang , Fawei Zhu , Zhanxian Li , Mingming Yu . Fluorescent probes reveal the differential impact of ferroptosis inhibition on drug-induced liver and kidney injury. Chinese Chemical Letters, 2026, 37(4): 111220-. doi: 10.1016/j.cclet.2025.111220
3. [3]
  Mingrui Zhang , Lingyu Jin , Yuda Zhu , Junfeng Kou , Bo Liu , Jing Chen , Xiaolin Zhong , Xianghua Wu , Junfeng Zhang , Wenxiu Ren . A near-infrared Ⅱ fluorescent dye based on oxanthracene: Real-time imaging of drug-induced acute liver injury and photothermal therapy for tumor. Chinese Chemical Letters, 2025, 36(10): 110772-. doi: 10.1016/j.cclet.2024.110772
4. [4]
  Ru Ding , Chunfei Wang , E Lv , Jingjing Zhao , Xucong Zhou , Yuanyi Li , Ying Wang , Bingxue Li , Lixia Zhang , Jin Zhou . A near-infrared fluorescent probe based on Au³⁺/Pd²⁺ complexes for glutathione sensing: Applications in acute liver injury, hepatocellular carcinoma, and ferroptosis exploration. Chinese Chemical Letters, 2025, 36(11): 111497-. doi: 10.1016/j.cclet.2025.111497
5. [5]
  Daili Liu , Changxiang Yu , Liyuan Lin , Zhidong Liu , Guiqian Fang , Qingqiang Yao , Qixin Chen , Xintian Shao . Berberrubine-mediated pH indicator response enhances the efficacy of hydroxycamptothecin by reversing lysosomal drug resistance. Chinese Chemical Letters, 2025, 36(9): 110718-. doi: 10.1016/j.cclet.2024.110718
6. [6]
  Meitong Wu , Ke Wu , Shumin Feng , Li Xu , Mi Lei , Jianmei Chen , Shuang Li , Mian Qin , Dahui Liu , Guoqiang Feng . A NIR and ratiometric fluorescent probe for quantitative detection of SO₂ derivatives in Chinese medicinal materials and bioimaging in vivo. Chinese Chemical Letters, 2026, 37(1): 110979-. doi: 10.1016/j.cclet.2025.110979
7. [7]
  Lixian Fu , Yiyun Tan , Yue Ding , Weixia Qing , Yong Wang . Water–soluble and polarity–sensitive near–infrared fluorescent probe for long–time specific cancer cell membranes imaging and C. Elegans label. Chinese Chemical Letters, 2024, 35(4): 108886-. doi: 10.1016/j.cclet.2023.108886
8. [8]
  Xing Tian , Di Wu , Wanheng Wei , Guifu Dai , Zhanxian Li , Benhua Wang , Mingming Yu . A lipid droplets-targetable fluorescent probe for polarity detection in cells of iron death, inflammation and fatty liver tissue. Chinese Chemical Letters, 2024, 35(6): 108912-. doi: 10.1016/j.cclet.2023.108912
9. [9]
  Fengzhi Wang , Ke Hu , Jinquan Chen , Zhubin Hu , Haitao Sun , Tony D. James , Yufang Xu , Xuhong Qian . Meta-amino substituted naphthalimides exhibit large charge transfer and strong N-H vibrations enabling use as ratiometric fluorescent probe. Chinese Chemical Letters, 2026, 37(1): 110971-. doi: 10.1016/j.cclet.2025.110971
10. [10]
  Jiao Chen , Zihan Zhang , Guojin Sun , Yudi Cheng , Aihua Wu , Zefan Wang , Wenwen Jiang , Fulin Chen , Xiuying Xie , Jianli Li . Benzo[4,5]imidazo[1,2-a]pyrimidine-based structure-inherent targeting fluorescent sensor for imaging lysosomal viscosity and diagnosis of lysosomal storage disorders. Chinese Chemical Letters, 2024, 35(11): 110050-. doi: 10.1016/j.cclet.2024.110050
11. [11]
  Han-Min Wang , Yan-Chen Li , Lu-Lu Sun , Ming-Ye Tang , Jia Liu , Jiahao Cai , Lei Dong , Jia Li , Yi Zang , Hai-Hao Han , Xiao-Peng He . Protein-encapsulated long-wavelength fluorescent probe hybrid for imaging lipid droplets in living cells and mice with non-alcoholic fatty liver. Chinese Chemical Letters, 2024, 35(11): 109603-. doi: 10.1016/j.cclet.2024.109603
12. [12]
  Xiangdong Lai , Tengfei Liu , Zengchao Guo , Yihan Wang , Jiang Xiao , Qingxiu Xia , Xiaohui Liu , Hui Jiang , Xuemei Wang . In situ formed fluorescent gold nanoclusters inhibit hair follicle regeneration in oxidative stress microenvironment via suppressing NFκB signal pathway. Chinese Chemical Letters, 2025, 36(2): 109762-. doi: 10.1016/j.cclet.2024.109762
13. [13]
  Qiuyue Lu , Min Shan , Jiaqi Yang , Zhongren Xu , Yueyue Lei , Wukun Liu . A dinuclear gold(I) complex with bis(N-heterocyclic carbene) ligands potentiated immune responses against liver cancer via ROS-driven endoplasmic reticulum stress and ferroptosis. Chinese Chemical Letters, 2025, 36(12): 110940-. doi: 10.1016/j.cclet.2025.110940
14. [14]
  Tiancong Shi , Xi Chen , Xiao Zhou , Hongyi Zhang , Fuping Han , Lihan Cai , Wen Sun , Jianjun Du , Jiangli Fan , Xiaojun Peng . Azaindole-based asymmetric pentamethine cyanine dye for mitochondrial pH detection and near-infrared ratiometric fluorescence imaging of mitophagy. Chinese Chemical Letters, 2025, 36(6): 110408-. doi: 10.1016/j.cclet.2024.110408
15. [15]
  Tengfei Zhang , Chen Han , Chiyuan Wei , Xing Wang , Zhihui Jia , Hong-Min Meng , Zhaohui Li . Ratiometric fluorescence probe using semiconducting polymer nanoparticles and carbon dots for predicting anti-cancer efficiency. Chinese Chemical Letters, 2026, 37(3): 111566-. doi: 10.1016/j.cclet.2025.111566
16. [16]
  Chuan-Zhi Ni , Ruo-Ming Li , Fang-Qi Zhang , Qu-Ao-Wei Li , Yuan-Yuan Zhu , Jie Zeng , Shuang-Xi Gu . A chiral fluorescent probe for molecular recognition of basic amino acids in solutions and cells. Chinese Chemical Letters, 2024, 35(10): 109862-. doi: 10.1016/j.cclet.2024.109862
17. [17]
  Tao Liu , Xuwei Han , Xueyi Sun , Weijie Zhang , Ke Gao , Runan Min , Yuting Tian , Caixia Yin . An activated fluorescent probe to monitor NO fluctuation in Parkinson’s disease. Chinese Chemical Letters, 2025, 36(3): 110170-. doi: 10.1016/j.cclet.2024.110170
18. [18]
  Wei GAO , Meiqi SONG , Xuan REN , Jianliang BAI , Jing SU , Jianlong MA , Zhijun WANG . A self-calibrating fluorescent probe for the selective detection and bioimaging of HClO. Chinese Journal of Inorganic Chemistry, 2025, 41(6): 1173-1182. doi: 10.11862/CJIC.20250112
19. [19]
  Lei ZHANG , Cheng HE , Yang JIAO . An azo-based fluorescent probe for the detection of hypoxic tumor cells. Chinese Journal of Inorganic Chemistry, 2025, 41(6): 1162-1172. doi: 10.11862/CJIC.20250081
20. [20]
  Qian Pang , Fangjun Huo , Yongkang Yue , Caixia Yin . ONOO⁻ and viscosity dual-response fluorescent probe for arthritis imaging in vivo. Chinese Chemical Letters, 2025, 36(9): 110713-. doi: 10.1016/j.cclet.2024.110713

Get Citation

PDF

XML

Metrics

PDF Downloads(8)
Abstract views(1648)
HTML views(106)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142