Citation: Lijuan Gui, Kaizhen Wang, Yuxin Wang, Jun Yan, Xian Liu, Jingxuan Guo, Ji Liu, Dawei Deng, Haiyan Chen, Zhenwei Yuan. Monitoring the pH fluctuation of lysosome under cell stress using a near-infrared ratiometric fluorescent probe[J]. Chinese Chemical Letters, ;2023, 34(3): 107586. doi: 10.1016/j.cclet.2022.06.009 shu

Monitoring the pH fluctuation of lysosome under cell stress using a near-infrared ratiometric fluorescent probe

Lijuan Gui ^{a, 1} ,
Kaizhen Wang ^{a, 1} ,
Yuxin Wang ^a ,
Jun Yan ^a ,
Xian Liu ^b ,
Jingxuan Guo ^a ,
Ji Liu ^a ,
Dawei Deng ^{a, *,} ,
Haiyan Chen ^{a, *,} ,
Zhenwei Yuan ^{a, *,}

a.
Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 210009, China
b.
Department of Health Technology and Informatics, Faculty of Health and Social Sciences, The Hong Kong Polytechnic University, Hong Kong, China

dengdawei@cpu.edu.cn

chenhaiyan@cpu.edu.cn

yuanzhenwei@cpu.edu.cn

Received Date: 14 March 2022
Revised Date: 1 June 2022
Accepted Date: 5 June 2022
Available Online: 10 June 2022

Figures(6)

Cell stress responses are associated with numerous diseases including diabetes, neurodegenerative diseases, and cancer. Several events occur under cell stress, in which, are protein expression and organelle-specific pH fluctuation. To understand the lysosomal pH variation under cell stress, a novel NIR ratiometric pH-responsive fluorescent probe (BLT) with lysosomes localization capability was developed. The quinoline ring of BLT combined with hydrogen ion which triggered the rearrangement of π electrons conjugated at low pH medium, meanwhile, the absorption and fluorescent spectra of BLT showed a red-shifts, which gived a ratiometric signal. Moreover, the probe BLT with a suitable pK_a value has the potential to discern changes in lysosomal pH, either induced by heat stress or oxidative stress or acetaminophen-induced (APAP) injury stress. Importantly, this ratiometric fluorescent probe innovatively tracks pH changes in lysosome in APAP-induced liver injury in live cells, mice, and zebrafish. The probe BLT as a novel fluorescent probe possesses important value for exploring lysosomal-associated physiological varieties of drug-induced hepatotoxicity.
- APAP-induced liver injury,
- Ratiometric fluorescent probe,
- Lysosomal pH,
- Cell stress
1. [1]
  S. Fulda, A.M. Gorman, O. Hori, A. Samali, Int. J. Cell. Biol.(2010) 214074. doi: 10.1155/2010/214074
2. [2]
  Y. Ishida, S. Nayak, J.A. Mindell, et al., J. Gen. Physiol. 141 (2013) 705-720. doi: 10.1085/jgp.201210930
3. [3]
  G.H. Sun-Wada, Y. Wada, M. Futai, Biochim. Biophys. Acta 1658 (2004) 106-114. doi: 10.1016/j.bbabio.2004.04.013
4. [4]
  J.R. Casey, S. Grinstein, J. Orlowski, Mol. Cell Biol. 11 (2010) 50-61. doi: 10.1038/nrm2820
5. [5]
  X. Liu, Y. Su, H. Tian, et al., Anal. Chem. 89 (2017) 7038-7045. doi: 10.1021/acs.analchem.7b00754
6. [6]
  X. Shi, N. Yan, G. Niu, et al., Chem. Sci. 11 (2020) 3152-3163. doi: 10.1039/c9sc06226b
7. [7]
  R.E. Lawrence, R. Zoncu, Nat. Cell Biol. 21 (2019) 133-142. doi: 10.1038/s41556-018-0244-7
8. [8]
  S.M. Davidson, M.G. Vander Heiden, Annu. Rev. Pharmacool. Toxicol. 57 (2017) 481-507. doi: 10.1146/annurev-pharmtox-010715-103101
9. [9]
  H. Hou, Y. Zhao, C. Li, et al., Sci. Rep. 7 (2017) 1759. doi: 10.1038/s41598-017-01956-1
10. [10]
  B.A. Webb, M. Chimenti, M.P. Jacobson, et al., Nat. Rev. Cancer 11 (2011) 671-677. doi: 10.1038/nrc3110
11. [11]
  G. Kroemer, M. Jäättelä, Nat. Rev. Cancer 5 (2005) 886-897. doi: 10.1038/nrc1738
12. [12]
  J. Han, K. Burgess, Chem. Rev. 110 (2010) 2709-2728. doi: 10.1021/cr900249z
13. [13]
  Y. Sun, X. Zhou, L. Sun, et al., Chin. Chem. Lett. 33 (2022) 4229–4232. doi: 10.1016/j.cclet.2022.01.087
14. [14]
  Z. Yuan, J. Chen, Q. Zhou, et al., Chin. Chem. Lett. 32 (2021) 1803-1808. doi: 10.1016/j.cclet.2020.12.012
15. [15]
  H. Ogasawara, Y. Tanaka, M. Taki, S. Yamaguchi, Chem. Sci. 12 (2021) 7902-7907. doi: 10.1039/d1sc01705e
16. [16]
  E.A. Owens, M. Henary, G. El Fakhri, et al., Acc. Chem. Res. 49 (2016) 1731-1740. doi: 10.1021/acs.accounts.6b00239
17. [17]
  C. Staudinger, S.M. Borisov, Methods Appl. Fluoresc. 3 (2015) 042005. doi: 10.1088/2050-6120/3/4/042005
18. [18]
  G. Niu, P. Zhang, W. Liu, et al., Anal. Chem. 89 (2017) 1922-1929. doi: 10.1021/acs.analchem.6b04417
19. [19]
  S.I. Reja, M. Minoshima, Y. Hori, et al., Chem. Sci. 12 (2020) 3437-3447. doi: 10.1039/d0sc04792a
20. [20]
  L. Yuan, W. Lin, S. Zhao, et al., J. Am. Chem. Soc. 134 (2012) 13510-13523. doi: 10.1021/ja305802v
21. [21]
  Q. Xia, S. Feng, J. Hong, et al., Talanta 228 (2021) 122184. doi: 10.1016/j.talanta.2021.122184
22. [22]
  T. Zhang, F. Huo, W. Zhang, et al., Sens. Actuators B: Chem. 345 (2021) 130393. doi: 10.1016/j.snb.2021.130393
23. [23]
  P. Ning, L. Hou, Y. Feng, et al., Chem. Commun. 55 (2019) 1782-1785. doi: 10.1039/c8cc09517e
24. [24]
  J. Ge, L. Fan, K. Zhang, et al., Sens. Actuat. B: Chem. 262 (2018) 913-921. doi: 10.1016/j.snb.2018.02.082
25. [25]
  G. Song, D. Jiang, L. Wang, et al., Chin. Chem. Lett. 33 (2022) 339-343. doi: 10.1016/j.cclet.2021.06.076
26. [26]
  Q. Wan, S. Chen, W. Shi, et al., Angew. Chem. Int. Ed. 53 (2014) 10916-10920. doi: 10.1002/anie.201405742
27. [27]
  H. Zhu, J. Fan, Q. Xu, et al., Chem. Commun. 48 (2012) 11766-11768. doi: 10.1039/c2cc36785h
28. [28]
  X. Chen, Q. Chen, M. Chen, et al., Sens. Actuators B: Chem. 329 (2021) 129104. doi: 10.1016/j.snb.2020.129104
29. [29]
  G. -J. Mao, Z. -Z. Liang, G. -Q. Gao, et al., Anal. Chim. Acta 1092 (2019) 117-125. doi: 10.1016/j.aca.2019.09.053
30. [30]
  D. Cheng, J. Peng, Y. Lv, et al., J. Am. Chem. Soc. 141 (2019) 6352-63 doi: 10.1021/jacs.9b01374
31. [31]
  D. Cheng, W. Xu, L. Yuan, et al., Anal. Chem. 89 (2017) 7693-7700. doi: 10.1021/acs.analchem.7b01671
32. [32]
  Y. Li, X. Xie, X. e. Yang, et al., Chem. Sci. 8 (2017) 4006-4011. doi: 10.1039/C7SC00303J
33. [33]
  Y. Liu, J. Zhou, L. Wang, et al., J. Am. Chem. Soc. 138 (2016) 12368-12374. doi: 10.1021/jacs.6b04048
34. [34]
  Y. Yue, F. Huo, X. Li, et al., Org. Lett. 19 (2017) 82-85. doi: 10.1021/acs.orglett.6b03357
35. [35]
  M.H. Lee, J.H. Han, J.H. Lee, et al., Angew. Chem. Int. Ed. 52 (2013) 6206-6209. doi: 10.1002/anie.201301894
1. [1]
  Xing Tian , Di Wu , Wanheng Wei , Guifu Dai , Zhanxian Li , Benhua Wang , Mingming Yu . A lipid droplets-targetable fluorescent probe for polarity detection in cells of iron death, inflammation and fatty liver tissue. Chinese Chemical Letters, 2024, 35(6): 108912-. doi: 10.1016/j.cclet.2023.108912
2. [2]
  Lixian Fu , Yiyun Tan , Yue Ding , Weixia Qing , Yong Wang . Water–soluble and polarity–sensitive near–infrared fluorescent probe for long–time specific cancer cell membranes imaging and C. Elegans label. Chinese Chemical Letters, 2024, 35(4): 108886-. doi: 10.1016/j.cclet.2023.108886
3. [3]
  Chuan-Zhi Ni , Ruo-Ming Li , Fang-Qi Zhang , Qu-Ao-Wei Li , Yuan-Yuan Zhu , Jie Zeng , Shuang-Xi Gu . A chiral fluorescent probe for molecular recognition of basic amino acids in solutions and cells. Chinese Chemical Letters, 2024, 35(10): 109862-. doi: 10.1016/j.cclet.2024.109862
4. [4]
  Bin Fang , Jiaqi Yang , Limin Wang , Haoqin Li , Jiaying Guo , Jiaxin Zhang , Qingyuan Guo , Bo Peng , Kedi Liu , Miaomiao Xi , Hua Bai , Li Fu , Lin Li . A mitochondria-targeted H₂S-activatable fluorogenic probe for tracking hepatic ischemia-reperfusion injury. Chinese Chemical Letters, 2024, 35(6): 108913-. doi: 10.1016/j.cclet.2023.108913
5. [5]
  Linfang Wang , Jing Liu , Minghao Ren , Wei Guo . A highly sensitive fluorescent HClO probe for discrimination between cancerous and normal cells/tissues. Chinese Chemical Letters, 2024, 35(6): 108945-. doi: 10.1016/j.cclet.2023.108945
6. [6]
  Yudi Cheng , Xiao Wang , Jiao Chen , Zihan Zhang , Jiadong Ou , Mengyao She , Fulin Chen , Jianli Li . A near-infrared fluorescent probe for visualizing transformation pathway of Cys/Hcy and H₂S and its applications in living system. Chinese Chemical Letters, 2024, 35(5): 109156-. doi: 10.1016/j.cclet.2023.109156
7. [7]
  Chuanfeng Fan , Jian Gao , Yingkai Gao , Xintong Yang , Gaoning Li , Xiaochun Wang , Fei Li , Jin Zhou , Haifeng Yu , Yi Huang , Jin Chen , Yingying Shan , Li Chen . A non-peptide-based chymotrypsin-targeted long-wavelength emission fluorescent probe with large Stokes shift and its application in bioimaging. Chinese Chemical Letters, 2024, 35(10): 109838-. doi: 10.1016/j.cclet.2024.109838
8. [8]
  Lei Shen , Hongmei Liu , Ming Jin , Jinchao Zhang , Caixia Yin , Shuxiang Wang , Yutao Yang . “Three-in-one” strategy of trifluoromethyl regulated blood-brain barrier permeable fluorescent probe for peroxynitrite and antiepileptic evaluation of edaravone. Chinese Chemical Letters, 2024, 35(10): 109572-. doi: 10.1016/j.cclet.2024.109572
9. [9]
  Xuejian Xing , Pan Zhu , E Pang , Shaojing Zhao , Yu Tang , Zheyu Hu , Quchang Ouyang , Minhuan Lan . D-A-D-structured boron-dipyrromethene with aggregation-induced enhanced phototherapeutic efficiency for near-infrared fluorescent and photoacoustic imaging-guided synergistic photodynamic and photothermal cancer therapy. Chinese Chemical Letters, 2024, 35(10): 109452-. doi: 10.1016/j.cclet.2023.109452
10. [10]
  Yunan Yuan , Zhimin Luo , Jie Chen , Chaoliang He , Kai Hao , Huayu Tian . Constructing thermoresponsive PNIPAM-based microcarriers for cell culture and enzyme-free cell harvesting. Chinese Chemical Letters, 2024, 35(7): 109549-. doi: 10.1016/j.cclet.2024.109549
11. [11]
  Jianye Kang , Xinyu Yang , Xuhao Yang , Jiahui Sun , Yuhang Liu , Shutao Wang , Wenlong Song . Carbon dots-enhanced pH-responsive lubricating hydrogel based on reversible dynamic covalent bondings. Chinese Chemical Letters, 2024, 35(5): 109297-. doi: 10.1016/j.cclet.2023.109297
12. [12]
  Shuang Li , Jiayu Sun , Guocheng Liu , Shuo Zhang , Zhong Zhang , Xiuli Wang . A new Keggin-type polyoxometallate-based bifunctional catalyst for trace detection and pH-universal photodegradation of phenol. Chinese Chemical Letters, 2024, 35(8): 109148-. doi: 10.1016/j.cclet.2023.109148
13. [13]
  Rui Wang , He Qi , Haijiao Zheng , Qiong Jia . Light/pH dual-responsive magnetic metal-organic frameworks composites for phosphorylated peptide enrichment. Chinese Chemical Letters, 2024, 35(7): 109215-. doi: 10.1016/j.cclet.2023.109215
14. [14]
  Yan Liu , Yang Wang , Jiayi Zhu , Xuxian Su , Xudong Lin , Liang Xu , Xiwen Xing . Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells. Chinese Chemical Letters, 2024, 35(9): 109427-. doi: 10.1016/j.cclet.2023.109427
15. [15]
  Di WU , Ruimeng SHI , Zhaoyang WANG , Yuehua SHI , Fan YANG , Leyong ZENG . Construction of pH/photothermal dual-responsive delivery nanosystem for combination therapy of drug-resistant bladder cancer cell. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1679-1688. doi: 10.11862/CJIC.20240135
16. [16]
  Simin Wei , Yaqing Yang , Junjie Li , Jialin Wang , Jinlu Tang , Ningning Wang , Zhaohui Li . The Mn/Yb/Er triple-doped CeO₂ nanozyme with enhanced oxidase-like activity for highly sensitive ratiometric detection of nitrite. Chinese Chemical Letters, 2024, 35(6): 109114-. doi: 10.1016/j.cclet.2023.109114
17. [17]
  Kun-Heng Li , Hong-Yang Zhao , Dan-Dan Wang , Ming-Hui Qi , Zi-Jian Xu , Jia-Mi Li , Zhi-Li Zhang , Shi-Wen Huang . Mitochondria-targeted nano-AIEgens as a powerful inducer for evoking immunogenic cell death. Chinese Chemical Letters, 2024, 35(5): 108882-. doi: 10.1016/j.cclet.2023.108882
18. [18]
  Yang Liu , Yan Liu , Kaiyin Yang , Zhiruo Zhang , Wenbo Zhang , Bingyou Yang , Hua Li , Lixia Chen . A selective HK2 degrader suppresses SW480 cancer cell growth by degrading HK2. Chinese Chemical Letters, 2024, 35(8): 109264-. doi: 10.1016/j.cclet.2023.109264
19. [19]
  Boran Cheng , Lei Cao , Chen Li , Fang-Yi Huo , Qian-Fang Meng , Ganglin Tong , Xuan Wu , Lin-Lin Bu , Lang Rao , Shubin Wang . Fluorine-doped carbon quantum dots with deep-red emission for hypochlorite determination and cancer cell imaging. Chinese Chemical Letters, 2024, 35(6): 108969-. doi: 10.1016/j.cclet.2023.108969
20. [20]
  Jing Chen , Peisi Xie , Pengfei Wu , Yu He , Zian Lin , Zongwei Cai . MALDI coupled with laser-postionization and trapped ion mobility spectrometry contribute to the enhanced detection of lipids in cancer cell spheroids. Chinese Chemical Letters, 2024, 35(4): 108895-. doi: 10.1016/j.cclet.2023.108895

Get Citation

PDF

XML

Metrics

PDF Downloads(6)
Abstract views(276)
HTML views(30)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142