Citation: Kehua Xu, Jingjing Zhang, Wenrui Ma, Hui Huang, Shiqiang Yan, Li Wang, Weijia Zhang. A facile cell culture device for studying nuclear and mitochondrial response of endothelial cells to hydrostatic pressure[J]. Chinese Chemical Letters, ;2023, 34(3): 107486. doi: 10.1016/j.cclet.2022.04.084 shu

A facile cell culture device for studying nuclear and mitochondrial response of endothelial cells to hydrostatic pressure

Kehua Xu ^a ,
Jingjing Zhang ^a ,
Wenrui Ma ^b ,
Hui Huang ^{a, c} ,
Shiqiang Yan ^a ,
Li Wang ^a ,
Weijia Zhang ^{a, b, c, *,}

a.
Institutes of Biomedical Sciences and Shanghai Institute of Infectious Disease and Biosecurity, Shanghai Medical College, Fudan University, Shanghai 200032, China
b.
Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China
c.
The State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200438, China

weijiazhang@fudan.edu.cn

Received Date: 19 February 2022
Revised Date: 1 April 2022
Accepted Date: 29 April 2022
Available Online: 2 May 2022

Figures(5)

There is no clear consensus regarding how cells respond to hydrostatic pressure. This is largely attributable to the high heterogeneity among cell types and the diverse custom-made devices used in previous studies. The aim of this work was to develop a facile device that could mimic various pressure environments and then delineate the cellular response to pressure stimulus. The device described here achieved both stable and periodic pressurization without oxygen deprivation. The biological utility of the device was assessed using human umbilical vein endothelial cells. We found more stereoscopic nuclear morphology and re-distribution of lamin A/C under high hydrostatic pressure compared to control cells. Mass spectrometry-based proteomics analysis showed significant changes in mitochondria-related pathways. Western blot analysis confirmed that high hydrostatic pressure induced a tendency toward mitochondrial fusion. Increased mitochondrial activity was observed as well. In conclusion, this device can be readily applied in biological research and extend our understanding of cellular mechano-sensation and the associated changes in mitochondrial behaviors.
- Cell culture device,
- Hydrostatic pressure,
- Human umbilical vein endothelial cells,
- Mitochondrial dynamics,
- Mitochondrial fusion,
- Mitochondrial fission
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