Citation: Wenchao Niu, Zhongying Du, Chunyu Zhang, Deting Xu, Jiaojiao Li, Minghui Sun, Liyuan Wu, Haodong Yao, Lina Zhao, Xueyun Gao. Broken electron transfer pathway in enzyme: Gold clusters inhibiting TrxR1/Trx via cell studies and theory simulations[J]. Chinese Chemical Letters, ;2022, 33(7): 3488-3491. doi: 10.1016/j.cclet.2022.04.004 shu

Broken electron transfer pathway in enzyme: Gold clusters inhibiting TrxR1/Trx via cell studies and theory simulations

Wenchao Niu ^{a, b} ,
Zhongying Du ^a ,
Chunyu Zhang ^a ,
Deting Xu ^{b, c} ,
Jiaojiao Li ^a ,
Minghui Sun ^{b, c} ,
Liyuan Wu ^b ,
Haodong Yao ^b ,
Lina Zhao ^{b, c, *,} ,
Xueyun Gao ^{a, *,}

a.
Department of Chemistry and Biology, Beijing University of Technology, Beijing 100124, China
b.
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China
c.
University of Chinese Academy of Sciences, Beijing 100049, China

linazhao@ihep.ac.cn

gaoxy@ihep.ac.cn

Received Date: 28 January 2022
Revised Date: 1 April 2022
Accepted Date: 1 April 2022
Available Online: 5 April 2022

Figures(4)

Thioredoxin reductase 1 (TrxR1) is over activity in tumor cell to maintain their redox balance. Although gold clusters have great potential in antitumor drug as they could well inhibit TrxR1, the molecular mechanism has not been disclosed yet. In this work, we revealed gold clusters can well inhibit the activity of TrxR1 in lung tumor cells and further disclosed the inhibition mechanism by using computational simulation methods. We firstly inferred the binding sites of gold in the hydrophobic cavities on TrxR1. The simulation results show that the gold ion (released from Au cluster) interact with –SH of Cys189 in TrxR1, this greatly increase the distance between the C-terminal redox center of TrxR1 and the Trx redox center, thereby destroy the electron transfer pathway between them. Our electron transfer destroying mechanism is different from the previous hypothesis that gold binds to the Sec498 of TrxR1 which has never been proved by experimental and theory studies. This work provides a new understanding of the gold clusters to inhibit TrxR1 activity.
- Enzyme inhibition,
- Electron transfer,
- Thioredoxin reductase 1,
- Gold clusters,
- Molecular dynamics,
- Density functional theory
1. [1]
  A. Holmgren, C. Johansson, C. Berndt, et al., Biochem. Soc. Trans.33 (2005) 1375–1377. doi: 10.1042/BST0331375
2. [2]
  M. Selenius, A.K. Rundlof, E. Olm, A.P. Fernande, M. Bjornstedt, Antioxid. Redox. Sign. 12 (2010) 867–880. doi: 10.1089/ars.2009.2884
3. [3]
  S. Urig, K. Fritz-Wolf, R. Réau, et al., Angew. Chem. Int. Ed. 45 (2006) 1881–1886. doi: 10.1002/anie.200502756
4. [4]
  A. Holmgren, M. Bjornstedt., Methods Enzymol. 252 (1995) 199–208.
5. [5]
  S.J.A. Elias, Biochim. Biophys. Acta 1790 (2009) 495–526. doi: 10.1016/j.bbagen.2009.01.014
6. [6]
  C.H. Lillig, A. Holmgren, Antioxid. Redox. Sign. 9 (2007) 25–47. doi: 10.1089/ars.2007.9.25
7. [7]
  D. Wang, S.J. Lippard, Nat. Rev. Drug. Discov. 4 (2005) 307–320. doi: 10.1038/nrd1691
8. [8]
  E.S.J. Arnér, H. Nakamura, T. Sasada, et al., Free Radicals Biol. Med. 31 (2001) 1170–1178. doi: 10.1016/S0891-5849(01)00698-0
9. [9]
  J. Lu, L.V. Papp, J.G. Fang, et al., Cancer Res. 66 (2006) 4410–4418. doi: 10.1158/0008-5472.CAN-05-3310
10. [10]
  R. Zhao, A. Holmgren, J. Biol. Chem. 277 (2002) 39456–39462. doi: 10.1074/jbc.M206452200
11. [11]
  B.R. You, W.H. Park, Int. J. Oncol. 48 (2016) 2197–2204. doi: 10.3892/ijo.2016.3402
12. [12]
  A. Jastrzab, E. Skrzydlewska, J. Enzyme Inhib. Med. Chem. 36 (2021) 362–371. doi: 10.1080/14756366.2020.1867121
13. [13]
  Q. Cheng, T. Sandalova, Y. Lindqvist, E.S.J. Arner, J. Biol. Chem. 284 (2009) 3998–4008. doi: 10.1074/jbc.M807068200
14. [14]
  J.J. Jia, W.S. Geng, Z.Q. Wang, L. Chen, X.S. Zeng, Cancer Chemoth. Pharm. 84 (2019) 453–470. doi: 10.1007/s00280-019-03869-4
15. [15]
  D. Parsonage, F. Sheng, K. Hirata, et al., J. Struct. Biol. 194 (2016) 180–190. doi: 10.1016/j.jsb.2016.02.015
16. [16]
  F. Angelucci, A.A. Sayed, D.L. Williams, et al., J. Biol. Chem. 284 (2009) 28977–28985. doi: 10.1074/jbc.M109.020701
17. [17]
  K. Fritz-Wolf, S. Kehr, M. Stumpf, S. Rahlfs, K. Becker, Nat. Commun. 2 (2011) 1–8.
18. [18]
  S.W. Zeng, K.T. Yong, I. Roy, et al., Plasmonics 6 (2011) 491–506. doi: 10.1007/s11468-011-9228-1
19. [19]
  B.Z. Zheng, L. Qian, H.Y. Yuan, et al., Talanta 82 (2010) 177–183. doi: 10.1016/j.talanta.2010.04.014
20. [20]
  M.R. Hormozi-Nezhad, P. Karami, H. Robatjazi, RSC Adv. 3 (2013) 7726–7732. doi: 10.1039/c3ra40280k
21. [21]
  Q.Z. Wu, H.Q. Cao, Q.Y. Luan, et al., Inorg. Chem. 47 (2008) 5882–5888. doi: 10.1021/ic8002228
22. [22]
  J. Zhai, L.N. Zhao, L.N. Zheng, et al., ACS Omega 2 (2017) 276–282. doi: 10.1021/acsomega.6b00390
23. [23]
  R. Liu, Y.L. Wang, Q. Yuan, et al., Chem. Commun. 50 (2014) 10687–10690. doi: 10.1039/C4CC03320E
24. [24]
  X.C. Zhang, R. Liu, Q. Yuan, et al., ACS Nano 12 (2018) 11139–11151. doi: 10.1021/acsnano.8b05584
25. [25]
  V.H. Teixeira, A.S.C. Capacho, M. Machuqueiro, Proteins 84 (2016) 1836–1843. doi: 10.1002/prot.25166
26. [26]
  M. Fereidoonnezhad, H.A. Mirsadeghi, S. Abedanzadeh, et al., New J. Chem. 43 (2019) 13173–13182. doi: 10.1039/c9nj02502b
27. [27]
  R. Rubbiani, T.N. Zehnder, C. Mari, et al., ChemMedChem 9 (2014) 2781–2790. doi: 10.1002/cmdc.201402446
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2. [2]
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