Citation: Long Zhao, Farid Ahmed, Hai Xiong. An excimer 'ONOFF' switch based on telomeric G-quadruplex and rGO for trace thrombin detection[J]. Chinese Chemical Letters, ;2022, 33(9): 4243-4247. doi: 10.1016/j.cclet.2022.02.048 shu

An excimer 'ONOFF' switch based on telomeric G-quadruplex and rGO for trace thrombin detection

Long Zhao ^{a, b} ,
Farid Ahmed ^a ,
Hai Xiong ^{a, *,}

a.
Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China
b.
College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China

hai.xiong@szu.edu.cn

Received Date: 3 November 2021
Revised Date: 17 February 2022
Accepted Date: 18 February 2022
Available Online: 22 February 2022

Figures(4)

In the recent decade, GO has emerged as an amazing 2D nanomaterial for developing DNA-based biosensors due to its fluorescence quenching properties, whereas similar research based on rGO was reported rarely. Herein, a novel multi-pyrene functionalized G-rich DNA probe based on the screened rGO showed much higher fluorescence quenching efficiency and excimer emission than that of universal GO. Different from the universal thrombin detection of the G4-forming aptamer-TBA(GGTTGGTGTGGTTGG), the original telomeric sequence is used in this study. The excimer emission "ON-OFF" switch amplified the response of thrombin detection is as low as 50 units. Furthermore, for four pyrene moieties that are sited in a crowded steric circumstance, the melting temperature (T_m) values and molecular dynamics simulations showed a positive effect on duplex G-quadruplex or _GDNA·_cDNA stability, without disturbing its helix structure.
- G-quadruplex,
- rGO,
- Multi-pyrene excimer,
- Biosensor,
- Thrombin detection
1. [1]
  C.H. Lu, H.H. Yang, C.L. Zhu, X. Chen, G.N. Chen, Angew. Chem. Int. Ed. 121 (2009) 4879–4881. doi: 10.1002/ange.200901479
2. [2]
  V. Georgakilas, J.N. Tiwari, K.C. Kemp, et al., Chem. Rev. 116 (2016) 5464–5519. doi: 10.1021/acs.chemrev.5b00620
3. [3]
  S. Xie, L. Ai, C. Cui, et al., ACS Appl. Mater. Interfaces 13 (2021) 9542–9560. doi: 10.1021/acsami.0c19562
4. [4]
  L. Feng, L. Wu, X. Qu, Adv. Mater. 25 (2013) 168–186. doi: 10.1002/adma.201203229
5. [5]
  D. Zhu, L. Wang, X. Xu, W. Jiang, Biosens. Bioelectro. 75 (2016) 155–160.
6. [6]
  K. Erickson, R. Erni, Z. Lee, et al., Adv. Mater. 22 (2010) 4467–4472. doi: 10.1002/adma.201000732
7. [7]
  Z. Wei, H. Li, J. Wu, et al., Chin. Chem. Lett. 31 (2020) 177–180. doi: 10.1016/j.cclet.2019.05.031
8. [8]
  N.D. Al-Khthami, T. Altalhi, M. Alsawat, et al., Mater. Express 11 (2021) 706–716. doi: 10.1166/mex.2021.1957
9. [9]
  Q.T. Ain, S.H. Haq, Mater. Express 10 (2020) 909–914. doi: 10.1166/mex.2020.1703
10. [10]
  Y. Shi, W.T. Huang, H.Q. Luo, N.B. Li, Chem. Comm. 47 (2011) 4676–4678. doi: 10.1039/c0cc05518b
11. [11]
  M. Liu, J. Song, S. Shuang, et al., ACS Nano 8 (2014) 5564–5573. doi: 10.1021/nn5007418
12. [12]
  M.E. Østergaard, P.J. Hrdlicka, Chem. Soc. Rev. 40 (2011) 5771–5788. doi: 10.1039/c1cs15014f
13. [13]
  G. Dougherty, J.R. Pilbrow, Int. J. Biochem. 16 (1984) 1179–1192. doi: 10.1016/0020-711X(84)90215-5
14. [14]
  K. Kalyanasundaram, J.K. Thomas, J. Am. Chem. Soc. 99 (1977) 2039–2044. doi: 10.1021/ja00449a004
15. [15]
  F.M. Winnik, Chem. Rev. 93 (1993) 587–614. doi: 10.1021/cr00018a001
16. [16]
  R.X.F. Ren, N.C. Chaudhuri, P.L. Paris, S. Rumney Iv, E.T. Kool, J. Am. Chem. Soc. 118 (1996) 7671–7678. doi: 10.1021/ja9612763
17. [17]
  Z. Wang, V. Enkelmann, F. Negri, K. Müllen, Angew. Chem. Int. Ed. 43 (2004) 1972–1975.
18. [18]
  Y.N. Teo, E.T. Kool, Chem. Rev. 112 (2012) 4221–4245. doi: 10.1021/cr100351g
19. [19]
  U.B. Christensen, E.B. Pedersen, Nucleic Acids Res. 30 (2002) 4918–4925. doi: 10.1093/nar/gkf624
20. [20]
  Y.J. Seo, H. Rhee, T. Joo, B.H. Kim, J. Am. Chem. Soc. 129 (2007) 5244–5247. doi: 10.1021/ja069069i
21. [21]
  M. Nakamura, Y. Murakami, K. Sasa, H. Hayashi, K. Yamana, J. Am. Chem. Soc. 130 (2008) 6904–6905. doi: 10.1021/ja801054t
22. [22]
  Y. Vyborna, M. Vybornyi, A.V. Rudnev, R. Häner, Angew. Chem. Int. Ed. 54 (2015) 7934–7938. doi: 10.1002/anie.201502066
23. [23]
  Y.W. Jun, D.L. Wilson, A.M. Kietrys, et al., Angew. Chem. Int. Ed. 132 (2020) 7520–7525. doi: 10.1002/ange.202001516
24. [24]
  Y.L. Wu, N.E. Horwitz, K.S. Chen, et al., Nat. Chem. 9 (2017) 466–472. doi: 10.1038/nchem.2689
25. [25]
  H. Tateishi-Karimata, K. Kawauchi, N. Sugimoto, J. Am. Chem. Soc. 140 (2017) 642–651. doi: 10.1021/jacs.7b09449
26. [26]
  K. Kawauchi, R. Urano, N. Kinoshita, et al., Genes (Basel)11 (2020) 1340. doi: 10.3390/genes11111340
27. [27]
  L. Wu, Y. Wang, X. Xu, et al., Chem. Rev. 121 (2021) 12035–12105. doi: 10.1021/acs.chemrev.0c01140
28. [28]
  C. Platella, C. Riccardi, D. Montesarchio, G.N. Roviello, D. Musumeci, Biochim. Biophys. Acta 1861 (2017) 1429–1447. doi: 10.1016/j.bbagen.2016.11.027
29. [29]
  Y. JunáSeo, I. JoonáLee, J. WuáYi, B. HyeanáKim, Chem. Comm. (2007) 2817–2819.
30. [30]
  C. Zhao, G. Qin, J. Niu, et al., Angew. Chem. Int. Ed. 133 (2021) 436–442. doi: 10.1002/ange.202011419
31. [31]
  P. Zhu, Y. Zhang, S. Xu, X. Zhang, Chin. Chem. Lett. 30 (2019) 58–62. doi: 10.1016/j.cclet.2018.02.003
32. [32]
  H. Cai, C. Zhou, Q. Yang, et al., Chin. Chem. Lett. 29 (2018) 531–534. doi: 10.1016/j.cclet.2017.09.010
33. [33]
  H. Xiong, P. Leonard, F. Seela, Bioconjug. Chem. 23 (2012) 856–870. doi: 10.1021/bc300013k
34. [34]
  B.G. Kim, J. Long, D.N. Dubins, T.V. Chalikian, J. Phys. Chem. B 120 (2016) 4963–4971. doi: 10.1021/acs.jpcb.6b03731
35. [35]
  Y. Kato, T. Ohyama, H. Mita, Y. Yamamoto, J. Am. Chem. Soc. 127 (2005) 9980–9981. doi: 10.1021/ja050191b
36. [36]
  E.M. Rezler, J. Seenisamy, S. Bashyam, et al., J. Am. Chem. Soc. 127 (2005) 9439–9447. doi: 10.1021/ja0505088
37. [37]
  M. Lu, Q. Guo, N.R. Kallenbach, Biochemistry 32 (1993) 598–601. doi: 10.1021/bi00053a027
38. [38]
  J.R. Wyatt, P.W. Davis, S.M. Freier, Biochemistry 35 (1996) 8002–8008. doi: 10.1021/bi960124h
39. [39]
  M. Manoharan, K.L. Tivel, M. Zhao, K. Nafisi, T.L. Netzel, J. Phys. Chem. 99 (1995) 17461–17472. doi: 10.1021/j100048a024
40. [40]
  Y.J. Seo, J.H. Ryu, B.H. Kim, Org. Lett. 7 (2005) 4931–4933. doi: 10.1021/ol0518582
41. [41]
  M.E. Østergaard, P. Kumar, B. Baral, et al., Chem. Eur. J. 17 (2011) 3157–3165. doi: 10.1002/chem.201002109
42. [42]
  M.L. Nierodzik, S. Karpatkin, Cancer Cell 10 (2006) 355–362. doi: 10.1016/j.ccr.2006.10.002
43. [43]
  H. Chang, L. Tang, Y. Wang, J. Jiang, J. Li, Anal. Chem. 82 (2010) 2341–2346. doi: 10.1021/ac9025384
44. [44]
  G. Shen, H. Zhang, C. Yang, Q. Yang, Y. Tang, Anal. Chem. 89 (2017) 548–551. doi: 10.1021/acs.analchem.6b04247
45. [45]
  N. Mohanty, V. Berry, Nano Lett. 8 (2008) 4469–4476. doi: 10.1021/nl802412n
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