Citation: Xiwei Li, Caiyun Liu, Na Gao, Wenlong Sheng, Baocun Zhu. A melatonin-based targetable fluorescent probe for screening of tumor cells and real-time imaging of glutathione fluctuations in tumor cells[J]. Chinese Chemical Letters, ;2022, 33(5): 2527-2531. doi: 10.1016/j.cclet.2021.11.080 shu

A melatonin-based targetable fluorescent probe for screening of tumor cells and real-time imaging of glutathione fluctuations in tumor cells

Xiwei Li ^a ,
Caiyun Liu ^{a, *,} ,
Na Gao ^a ,
Wenlong Sheng ^b ,
Baocun Zhu ^{a, *,}

a.
School of Water Conservancy and Environment, University of Jinan, Ji'nan 250022, China
b.
Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji'nan 250103, China

liucaiyun1982072@163.com

lcyzbc@163.com

Received Date: 20 September 2021
Revised Date: 23 November 2021
Accepted Date: 26 November 2021
Available Online: 1 December 2021
Fund Project: the National Natural Science Foundation of China 21777053the National Natural Science Foundation of China 22176070Shandong Province Higher Educational Youth Innovation Science and Technology Program 2019KJD005

Figures(3)

Glutathione (GSH) is a key maintainer of cellular redox balance and plays an important role in many physiological effects. For example, GSH has been widely implicated in cancer initiation, progression and metastasis. Moreover, the concentrations of GSH in tumor cells can influence drug resistance. Given the serious harmfulness of cancer and the important roles of GSH in cancer, it has great significance to development probes for screening of tumor cells and real-time monitoring of GSH fluctuations in tumor cells. However, no targetable probe for reversible imaging of GSH in tumor cells has been reported. Herein, we constructed a melatonin-based targetable and reversible fluorescent probe (GR-MT) for screening of tumor cells and real-time imaging of GSH fluctuations in tumor cells. The probe uses coumarin as the skeleton, Michael addition reaction as the reaction mechanism, and melatonin as the targeted groups of tumor cells. The experimental results demonstrate this probe has many advantages including high selectivity, satisfactory sensitivity, excellent reversible ability, rapid reaction speed, and outstanding targetability of tumor cells. Therefore, this study provides a promising tool for tumor cells screening and real-time detection of GSH fluctuations in specific tumor cells.
- Glutathione,
- Reversible detection,
- Fluorescent probe,
- Tumor cells screening,
- Melatonin
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