Citation: Fen Yang, Da Zhang, Quanming Zhou, Mengchu Li, Chengliang Xie, Shaoyun Li, Xuan Wang, Wei Wang, Ying Guo, Qicai Xiao, Yong Wang, Liqian Gao. Peptides-modified polystyrene-based polymers as high-performance substrates for the growth and propagation of human embryonic stem cells[J]. Chinese Chemical Letters, ;2022, 33(6): 2901-2905. doi: 10.1016/j.cclet.2021.10.028 shu

Peptides-modified polystyrene-based polymers as high-performance substrates for the growth and propagation of human embryonic stem cells

Fen Yang ^{a, 1} ,
Da Zhang ^{a, 1} ,
Quanming Zhou ^{a, 1} ,
Mengchu Li ^a ,
Chengliang Xie ^a ,
Shaoyun Li ^a ,
Xuan Wang ^a ,
Wei Wang ^a ,
Ying Guo ^a ,
Qicai Xiao ^{a, *,} ,
Yong Wang ^{b, *,} ,
Liqian Gao ^{a, *,}

a.
School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen 518107, China
b.
Institute of Bioengineering and Bioimaging, Singapore 138669, Singapore

gaolq@mail.sysu.edu.cn

Georgeyywang@yahoo.com

xiaoqicai@mail.sysu.edu.cn

Received Date: 9 August 2021
Revised Date: 17 September 2021
Accepted Date: 11 October 2021
Available Online: 21 October 2021

Figures(4)

As human stem cells with the special pluripotency play important roles in the innovative drug discovery and regenerative medicine, development of extracellular matrix (ECM) mimetics or functional materials that can support stem cell growth and propagation is of high significance. Despite numerous efforts spent, one major limitation restricting the wide applications of stem cells to the clinical translation is the lack of efficient strategies for low cost and large-scale stem cell production under xeno-free culture conditions. Herein, we reported a new strategy with peptides-modified polystyrene-based polymers coated onto the surface of coverslips for the growth and reproduction of human embryonic stem cells (hESCs). The modified peptides are the active parts of proteins which has been shown to contribute to the pluripotent stem cell attachment or proliferation. The peptides were linked to the glass coverslips coated by the polymer materials via chemical crosslinking, and the composite substrates successfully maintain the long-term growth of HUES-7, H7 and DF699. Our study shows that the coating of polystyrene-derived polymer modified by our developed peptides is a good matrix for long-term growth and reproduction of stem cells. This polystyrene-derived polymer substrate can be produced in large scale and stored for a long time. The most important thing is that it can support the growth of undifferentiated human pluripotent stem cells (hPSCs) for more than ten passages, which could provide a new and relatively easy way to amplify hESCs in vitro.
- Extracellular matrix,
- Stem cell,
- Peptide,
- Coverslip,
- Polystyrene
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