Citation: Huimin Chen, Han Wang, Yongchun Wei, Maomao Hu, Bo Dong, Hongbao Fang, Qixin Chen. Super-resolution imaging reveals the subcellular distribution of dextran at the nanoscale in living cells[J]. Chinese Chemical Letters, ;2022, 33(4): 1865-1869. doi: 10.1016/j.cclet.2021.10.025 shu

Super-resolution imaging reveals the subcellular distribution of dextran at the nanoscale in living cells

Huimin Chen ^a ,
Han Wang ^{a, b} ,
Yongchun Wei ^a ,
Maomao Hu ^{a, b} ,
Bo Dong ^b ,
Hongbao Fang ^c ,
Qixin Chen ^{a, *,}

a.
Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji'nan 250062, China
b.
Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong University, Ji'nan 250012, China
c.
State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China

chenqixin@sdfmu.edu.cn

Received Date: 26 June 2021
Revised Date: 9 October 2021
Accepted Date: 11 October 2021
Available Online: 16 October 2021

Figures(4)

Theranostic visualization of dextran at the nanoscale is beneficial for understanding the bioregulatory mechanisms of this molecule. In this study, we applied structured illumination microscopy (SIM) to capture the distribution of Cy5-Dextran at different incubation periods in living cells. The results showed that Cy5-Dextran could be absorbed by HeLa cells. In addition, we clarified that Cy5-Dextran exhibited differential organelle distribution (lysosomal or mitochondrial) in a time-dependent manner. Moreover, lysosomal Cy5-Dextran localization was found to be independent of the autophagy process, while Cy5-Dextran localized to the mitochondria triggered a pro-apoptotic event, upregulating the levels of reactive oxygen species (ROS) to accelerate mitochondrial fragmentation. This work uses a visualized strategy to reveal the anti-tumor bioactivity of dextran, which was achieved by regulating apoptosis and autophagy.
- Dextran,
- Subcellular distribution,
- Super-resolution imaging,
- Structured illumination microscopy
1. [1]
  K.M.I. Bashir, J.S. Choi, Int. J. Mol. Sci. 18(2017) 1906. doi: 10.3390/ijms18091906
2. [2]
  R. Raveendran, G.S. Bhuvaneshwar, C.P. Sharma, Carbohydr. Polym. 137(2016) 497-507. doi: 10.1016/j.carbpol.2015.11.017
3. [3]
  J.G. Lee, Y.S. Kim, Y.J. Lee, et al., Nutrients 8(2016) 336. doi: 10.3390/nu8060336
4. [4]
  N. Wang, H. Liu, G. Liu, et al., Redox. Biol. 32(2020) 101495. doi: 10.1016/j.redox.2020.101495
5. [5]
  H. Xu, S. Zou, X. Xu, Oncotarget 8(2017) 86693-86709. doi: 10.18632/oncotarget.21411
6. [6]
  O. Vittorio, M. Brandl, G. Cirillo, et al., Oncotarget 7(2016) 47479-47493. doi: 10.18632/oncotarget.10201
7. [7]
  X. Duan, G. Zhao, X. Han, et al., Oncol. Rep. 46(2021) 124. doi: 10.3892/or.2021.8075
8. [8]
  K. Wang, W. Ma, Y. Xu, et al., Chin. Chem. Lett. 31(2020) 3149-3152. doi: 10.1016/j.cclet.2020.08.039
9. [9]
  M.M. Usaj, E.B. Styles, A.J. Verster, et al., Trends Cell Biol. 26(2016) 598-611. doi: 10.1016/j.tcb.2016.03.008
10. [10]
  Z. Liu, L.D. Lavis, E. Betzig, Mol. Cell 58(2015) 644-659. doi: 10.1016/j.molcel.2015.02.033
11. [11]
  X. Fu, W. Shang, S. Wang, et al., Chem. Commun. 53(2017) 3555-3558. doi: 10.1039/C6CC09431G
12. [12]
  X. Huang, J. Fan, L. Li, et al., Nat. Biotechnol. 36(2018) 451-459. doi: 10.1038/nbt.4115
13. [13]
  J. Hanne, H.J. Falk, F. Görlitz, et al., Nat. Commun. 6(2015) 7127. doi: 10.1038/ncomms8127
14. [14]
  B. Huang, W. Wang, M. Bates, X. Zhuang, Science 319(2008) 810-813. doi: 10.1126/science.1153529
15. [15]
  Q. Chen, C. Jin, X. Shao, et al., Small 14(2018) 1802166. doi: 10.1002/smll.201802166
16. [16]
  X. Li, J. Zheng, W. Liu, et al., Chin. Chem. Lett. 31(2020) 2937-2940. doi: 10.1016/j.cclet.2020.05.043
17. [17]
  Q. Chen, X. Shao, Z. Tian, et al., Nano Res. 12(2019).
18. [18]
  Y. Wei, L. Kong, H. Chen, et al., Chem. Eng. J. (2021) 132134.
19. [19]
  S. Liu, G. Ge, W. Dong, et al., Sens. Actuators B 253(2017) 1145-1151. doi: 10.1016/j.snb.2017.07.170
20. [20]
  R. Shukla, A. Goyal, Int. J. Biol. Macromol. 62(2013) 352-357. doi: 10.1016/j.ijbiomac.2013.09.043
21. [21]
  Q. Chen, X. Shao, M. Hao, et al., Biomaterials 250(2020) 120059. doi: 10.1016/j.biomaterials.2020.120059
22. [22]
  Q. Chen, H. Fang, X. Shao, et al., Nat. Commun. 11(2020) 6290. doi: 10.1038/s41467-020-20067-6
23. [23]
  M.B. Azad, Y. Chen, S.B. Gibson, Antioxid. Redox Signal. 11(2009) 777-790. doi: 10.1089/ars.2008.2270
24. [24]
  Y. Wen, F. Huo, C. Yin, Chin. Chem. Lett. 30(2019) 1834-1842. doi: 10.1016/j.cclet.2019.07.006
25. [25]
  C.C. Winterbourn, Free Radical Biol. Med. 80(2015) 164-170. doi: 10.1016/j.freeradbiomed.2014.08.017
26. [26]
  M. RedzaDutordoir, D.A. AverillBates, BBA-Molecular Cell Res. 1863(2016) 2977-2992.
27. [27]
  S.S. Ruth, E. Zvulun, Trends Cell Biol. 17(2007) 422-427. doi: 10.1016/j.tcb.2007.07.009
28. [28]
  AM. B, Yongqiang C., GS. B, Antioxid. Redox Signal. 11(2009) 777-790. doi: 10.1089/ars.2008.2270
29. [29]
  Y.C. Won, J. Juhee, G. Gyeongyun, et al., Int. J. Mol. Sci. 22(2020) 179. doi: 10.3390/ijms22010179
30. [30]
  H. Iwashita, H.T. Sakurai, N. Nagahora, et al., FEBS Lett. 592(2018) 559-567. doi: 10.1002/1873-3468.12979
31. [31]
  R. Shukla, A. Goyal, Int. J. Biol. Macromol. 62(2013) 352-357. doi: 10.1016/j.ijbiomac.2013.09.043
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2. [2]
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15. [15]
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