Citation: Shengtao Yao, Yue Liao, Rizhao Pan, Weiping Zhu, Yufang Xu, Yangyang Yang, Xuhong Qian. Programmed co-assembly of DNA-peptide hybrid microdroplets by phase separation[J]. Chinese Chemical Letters, ;2022, 33(3): 1545-1549. doi: 10.1016/j.cclet.2021.08.116 shu

Programmed co-assembly of DNA-peptide hybrid microdroplets by phase separation

Shengtao Yao ^a ,
Yue Liao ^a ,
Rizhao Pan ^a ,
Weiping Zhu ^a ,
Yufang Xu ^{a, *,} ,
Yangyang Yang ^{a, *,} ,
Xuhong Qian ^{a, b, *,}

a.
School of Pharmacy, Shanghai Key Laboratory of Chemical Biology, East China University of Science and Technology, Shanghai 200237, China
b.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology Shanghai 200237, China

yfxu@ecust.edu.cn

triyang@ecust.edu.cn

xhqian@ecust.edu.cn

Received Date: 2 July 2021
Revised Date: 26 August 2021
Accepted Date: 27 August 2021
Available Online: 2 September 2021

Figures(4)

Biopolymers, including DNA and peptides have been used as excellent self-assembling building blocks for programmable single-component or hybrid materials, due to their controlled molecular interactions. However, combining two assembling principles of DNA-based programmability and peptide-based specific molecular interactions for hybrid structures to microscale has not yet been achieved. In this study, we describe a hybrid microsystem that emerges from the co-assembly of DNA origami structure and short elastin-like polypeptide conjugated oligonucleotides, and initiates liquid-liquid phase separation to generate microdroplets upon heating above the transition temperature. Moreover, the hybrid microdroplets are capable for guest molecule trapping and perform bi-/tri-enzymatic cascades with rate enhancements as open "microreactors". Our programmed assembled DNA-peptide microsystem represents a new combination of DNA nanotechnology and peptide science and opens potential application routes toward life-inspired biomaterials.
- Self-assembly,
- Microdroplet,
- DNA origami,
- Phase separation,
- Elastin-like polypeptides
1. [1]
  F. Hong, F. Zhang, Y. Liu, H. Yan, Chem. Rev. 117 (2017) 12584-12640. doi: 10.1021/acs.chemrev.6b00825
2. [2]
  D.M. Raymond, B.L. Nilsson, Chem. Soc. Rev. 47 (2018) 3659-3720. doi: 10.1039/C8CS00115D
3. [3]
  T. MacCulloch, A. Buchberger, N. Stephanopoulos, Org. Biomol. Chem. 17 (2019) 1668-1682. doi: 10.1039/C8OB02436G
4. [4]
  Y.H. Dong, C. Yao, Y. Zhu, et al., Chem. Rev. 120 (2020) 9420-9481. doi: 10.1021/acs.chemrev.0c00294
5. [5]
  E. Michel, A. Pluckthun, O. Zerbe, Angew. Chem. Int. Ed. 57 (2018) 4576-4579. doi: 10.1002/anie.201713377
6. [6]
  Y.M. Wong, H. Masunaga, J.A. Chuah, et al., Biomacromolecules 17 (2016) 3375-3385. doi: 10.1021/acs.biomac.6b01169
7. [7]
  M. Kye, Y.B. Lim, Angew. Chem. Int. Ed. 55 (2016) 12003-12007. doi: 10.1002/anie.201605696
8. [8]
  M. Humenik, T. Scheibel, ACS Nano 8 (2014) 1342-1349. doi: 10.1021/nn404916f
9. [9]
  R. Merindol, S. Loescher, A. Samanta, A. Walther, Nat. Nanotechnol. 13 (2018) 730-738. doi: 10.1038/s41565-018-0168-1
10. [10]
  J. Deng, A. Walther, Chem 6 (2020) 3329-3343. doi: 10.1016/j.chempr.2020.09.022
11. [11]
  B.J. Jeon, D.T. Nguyen, O.A. Saleh, J. Phys. Chem. B 124 (2020) 8888-8895. doi: 10.1021/acs.jpcb.0c06911
12. [12]
  Y. Sato, T. Sakamoto, M. Takinoue, Sci. Adv. 6 (2020) eaba3471. doi: 10.1126/sciadv.aba3471
13. [13]
  T.R. Wilks, J. Bath, J.W. de Vries, et al., ACS Nano 7 (2013) 8561-8572. doi: 10.1021/nn402642a
14. [14]
  P.W.K. Rothemund, Nature 440 (2006) 297-302. doi: 10.1038/nature04586
15. [15]
  F. Wang, X.Q. Liu, I. Willner, Angew. Chem. Int. Ed. 54 (2015) 1098-1129. doi: 10.1002/anie.201404652
16. [16]
  Q. Jiang, C. Song, J. Nangreave, et al., J. Am. Chem. Soc. 134 (2012) 13396-13403. doi: 10.1021/ja304263n
17. [17]
  S. Gwo, H.Y. Chen, M.H. Lin, et al., Chem. Soc. Rev. 45 (2016) 5672-5716. doi: 10.1039/C6CS00450D
18. [18]
  H. Yu, T.T. Man, W. Ji, et al., Chin. Chem. Lett. 30 (2019) 175-178. doi: 10.1016/j.cclet.2018.04.020
19. [19]
  S. Hernandez-Ainsa, U.F. Keyser, Nanoscale 6 (2014) 14121-14132. doi: 10.1039/C4NR04094E
20. [20]
  J. Jin, E.G. Baker, C.W. Wood, et al., ACS Nano 13 (2019) 9927-9935. doi: 10.1021/acsnano.9b04251
21. [21]
  A. Buchberger, C.R. Simmons, N.E. Fahmi, et al., J. Am. Chem. Soc. 142 (2020) 1406-1416. doi: 10.1021/jacs.9b11158
22. [22]
  T. Jiang, T.A. Meyer, C. Modlin, et al., J. Am. Chem. Soc. 139 (2017) 14025-14028. doi: 10.1021/jacs.7b08087
23. [23]
  S. Roberts, M. Dzuricky, A. Chilkoti, FEBS Lett. 589 (2015) 2477-2486. doi: 10.1016/j.febslet.2015.08.029
24. [24]
  H. Zhao, V. Ibrahimova, E. Garanger, S. Lecommandoux, Angew. Chem. Int. Ed. 59 (2020) 11028-11036. doi: 10.1002/anie.202001868
25. [25]
  J.Y. Qin, J.D. Sloppy, K.L. Kiick, Sci. Adv. 6 (2020) eabd3033. doi: 10.1126/sciadv.abd3033
26. [26]
  B. Wang, R.Z. Pan, W.P. Zhu, et al., Soft Matter 17 (2021) 1184-1188. doi: 10.1039/D0SM01817A
27. [27]
  M.A. Goetzfried, K. Vogele, A. Muckl, et al., Small 15 (2019) 1903541. doi: 10.1002/smll.201903541
28. [28]
  Y.Y. Yang, S.W. Zhang, S.T. Yao, et al., Chem. Eur. J. 25 (2019) 5158-5162. doi: 10.1002/chem.201900221
29. [29]
  J.R. McDaniel, D.C. Radford, A. Chilkoti, Biomacromolecules 14 (2013) 2866-2872. doi: 10.1021/bm4007166
30. [30]
  X.M. Sun, Y. Li, Q. Yang, et al., Chin. Chem. Lett. 32 (2021) 1780-1784. doi: 10.1016/j.cclet.2020.12.041
31. [31]
  J.B. Li, X.M. Liu, L.K.E.A. Abdelmohsen, et al., Small 15 (2019) 1902893. doi: 10.1002/smll.201902893
32. [32]
  P. Heneberg, Antioxid. Redox Sign. 30 (2019) 415-442. doi: 10.1089/ars.2017.7255
33. [33]
  S.Y. Lunt, M.G. Vander Heiden, Annu. Rev. Cell Dev. Bi. 27 (2011) 441-464. doi: 10.1146/annurev-cellbio-092910-154237
34. [34]
  G.L. Ke, M.H. Liu, S.X. Jiang, et al., Angew. Chem. Int. Ed. 55 (2016) 7483-7486. doi: 10.1002/anie.201603183
35. [35]
  Y.H.R. Yang, J.L. Fu, S. Wootten, et al., ChemBioChem 19 (2018) 212-216. doi: 10.1002/cbic.201700613
36. [36]
  Y.H. Chen, G.L. Ke, Y.L. Ma, et al., J. Am. Chem. Soc. 140 (2018) 8990-8996. doi: 10.1021/jacs.8b05429
37. [37]
  J. Chen, R.J. Dai, B. Tong, S.Y. Xiao, W.W. Meng, Chin. Chem. Lett. 18 (2007) 10-12. doi: 10.1016/j.cclet.2006.11.009
38. [38]
  W.J. Qin, C.C. Xu, Y.F. Zhao, et al., Chin. Chem. Lett. 29 (2018) 1451-1455. doi: 10.1016/j.cclet.2018.04.007
39. [39]
  J. Zhou, W. Shi, L.H. Li, et al., Chem. Asian J. 11 (2016) 2719-2724. doi: 10.1002/asia.201600012
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