Citation: Juan Wang, Yuwei Sheng, Ying Yang, Xiaoxia Dai, Changjun You. Next-generation sequencing-based analysis of the effect of N⁶-methyldeoxyadenosine modification on DNA replication in human cells[J]. Chinese Chemical Letters, ;2022, 33(4): 2077-2080. doi: 10.1016/j.cclet.2021.08.066 shu

Next-generation sequencing-based analysis of the effect of N⁶-methyldeoxyadenosine modification on DNA replication in human cells

Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha 410082, China

xiaoxiad@hnu.edu.cn

changjun@hnu.edu.cn

Received Date: 6 July 2021
Revised Date: 10 August 2021
Accepted Date: 13 August 2021
Available Online: 19 August 2021

Figures(4)

N⁶-methyldeoxyadenosine (6mdA) modification is considered as a new epigenetic mark that may play important roles in various biological processes. However, it remains unclear about the effect of 6mdA on DNA replication in human cells. Herein, we combined next-generation sequencing with shuttle vector technology to explore how 6mdA affects the efficiency and accuracy of DNA replication in human cells. Our results showed that 6mdA neither blocked DNA replication nor induced mutations in human cells. Moreover, we found that the depletion of translesion synthesis DNA polymerase (Pol) κ, Pol η, Pol ι or Pol ζ did not significantly change the biological consequences of 6mdA during replication in human cells. The negligible impact of 6mdA on DNA replication is consistent with its potential role in epigenetic gene expression.
- -methyldeoxyadenosine,
- DNA replication,
- Next-generation sequencing,
- Shuttle vector technology,
- Translesion synthesis DNA polymerase
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

Next-generation sequencing-based analysis of the effect of N6-methyldeoxyadenosine modification on DNA replication in human cells

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通讯作者: 陈斌, bchen63@163.com

Next-generation sequencing-based analysis of the effect of N⁶-methyldeoxyadenosine modification on DNA replication in human cells