Citation: Xing Wei, Meng Yang, Ze Jiang, Jinhui Liu, Xuan Zhang, Mingli Chen, Jianhua Wang. A modular single-cell pipette microfluidic chip coupling to ETAAS and ICP-MS for single cell analysis[J]. Chinese Chemical Letters, ;2022, 33(3): 1373-1376. doi: 10.1016/j.cclet.2021.08.024 shu

A modular single-cell pipette microfluidic chip coupling to ETAAS and ICP-MS for single cell analysis

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, China

chenml@mail.neu.edu.cn

jianhuajrz@mail.neu.edu.cn

Received Date: 28 May 2021
Revised Date: 11 July 2021
Accepted Date: 6 August 2021
Available Online: 12 August 2021

Figures(4)

Accurate single-cell capture is a crucial step for single cell biological and chemical analysis. Conventional single-cell capturing often confront operational complexity, limited efficiency, cell damage, large scale but low accuracy, incompetence in the acquirement of nano-upgraded single-cell liquid. Flow cytometry has been widely used in large-scale single-cell detection, while precise single-cell isolation relies on both a precision operating platform and a microscope, which is not only extremely inefficient, but also not conducive to couple with modern analytical instruments. Herein, we develop a modular single-cell pipette (mSCP) microfluidic chip with high efficiency and strong applicability for accurate direct capture of single viable cell from cell suspensions into nanoliter droplets (30-1000 nL). The mSCP is used as a sampling platform for the detection of CdTe quantum dots in single cells with electrothermal atomic absorption spectrometry (ETAAS) for the first time. It also ensures precise single-cell sampling and detection by inductively coupled plasma mass spectrometry (ICP-MS).
- Single-cell pipette,
- Microfluidic chip,
- Single-cell capture,
- ETAAS,
- ICP-MS,
- CdTe QDs
1. [1]
  V. Almendro, A. Marusyk, K. Polyak, Annu. Rev. Pathol. Mech. Dis. 8(2013) 277-302. doi: 10.1146/annurev-pathol-020712-163923
2. [2]
  K. Zhang, X. Han, Y. Li, et al., J. Am. Chem. Soc. 136(2014) 10858-10861. doi: 10.1021/ja5053279
3. [3]
  C.E. Meacham, S.J. Morrison, Nature 501(2013) 328-337. doi: 10.1038/nature12624
4. [4]
  C. Ma, R. Fan, H. Ahmad, et al., Nat. Med. 17(2011) 738-743. doi: 10.1038/nm.2375
5. [5]
  K. Xu, G.S. Zhong, X.W. Zhuang, Science 339(2013) 452-456. doi: 10.1126/science.1232251
6. [6]
  V. Sanchez-Freire, A.D. Ebert, T. Kalisky, S.R. Quake, J.C. Wu, Nat. Protoc. 7(2012) 829-838. doi: 10.1038/nprot.2012.021
7. [7]
  V. Lecault, M. Vaninsberghe, S. Sekulovic, et al., Nat. Methods 8(2011) 581-586. doi: 10.1038/nmeth.1614
8. [8]
  X. Wei, D.H. Zheng, Y. Cai, et al., Anal. Chem. 90(2018) 14543-14550. doi: 10.1021/acs.analchem.8b04471
9. [9]
  A. Sarkar, S. Kolitz, D.A. Lauffenburger, J. Han, Nat. Commun. 5(2014) 3421. doi: 10.1038/ncomms4421
10. [10]
  P.C. Blainey, S.R. Quake, Nat. Methods 11(2014) 19-21. doi: 10.1038/nmeth.2783
11. [11]
  X. Wei, L.L. Hu, M.L. Chen, T. Yang, J.H. Wang, Anal. Chem. 88(2016) 12437-12444. doi: 10.1021/acs.analchem.6b03810
12. [12]
  L.A. Austin, B. Kang, M.A. El-Sayed, J. Am. Chem. Soc. 135(2013) 4688-4691. doi: 10.1021/ja4011145
13. [13]
  J.M. Camarillo, S. Swaminathan, N.A. Abshiru, et al., J. Am. Soc. Mass Spectrom. 30(2019) 2526-2534. doi: 10.1007/s13361-019-02255-x
14. [14]
  N.R. Munce, J. Li, P.R. Herman, L. Lilge, Anal. Chem. 76(2004) 4983-4989. doi: 10.1021/ac0496906
15. [15]
  W. Zhang, N. Li, L. Lin, H. Li, J.M. Lin, Anal. Chem. 93(2021) 6955-6960. doi: 10.1021/acs.analchem.0c05155
16. [16]
  V. Espina, J. Milia, G. Wu, S. Cowherd, L.A. Liotta, Methods Mol. Biol. 319(2006) 213-229. doi: 10.1007/978-1-59259-993-6_10
17. [17]
  X. Liu, Y. Jia, Z. Han, et al., Angew. Chem. Int. Ed. 60(2021) 12319-12322. doi: 10.1002/anie.202101293
18. [18]
  W.H. Tan, S. Takeuchi, Proc. Natl. Acad. Sci. U. S. A. 104(2007) 1146-1151. doi: 10.1073/pnas.0606625104
19. [19]
  S. Kobel, A. Valero, J. Latt, P. Renaud, M. Lutolf, Lab Chip 10(2010) 857-863. doi: 10.1039/b918055a
20. [20]
  K. Chung, C.A. Rivet, M.L. Kemp, H. Lu, Anal. Chem. 83(2011) 7044-7052. doi: 10.1021/ac2011153
21. [21]
  K. Chung, Y. Kim, J.S. Kanodia, et al., Nat Methods 8(2011) 171-176. doi: 10.1038/nmeth.1548
22. [22]
  K.K. Kabirov, I.M. Kapetanovic, A.V. Lyubimov, Chem. -Biol. Interact. 171(2008) 152-158. doi: 10.1016/j.cbi.2007.11.011
23. [23]
  Z. Ajtony, N. Szoboszlai, E.K. Suskó, et al., Talanta 76(2008) 627-634. doi: 10.1016/j.talanta.2008.04.014
1. [1]
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2. [2]
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3. [3]
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4. [4]
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5. [5]
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6. [6]
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7. [7]
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8. [8]
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9. [9]
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10. [10]
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11. [11]
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12. [12]
  Jing Chen , Peisi Xie , Pengfei Wu , Yu He , Zian Lin , Zongwei Cai . MALDI coupled with laser-postionization and trapped ion mobility spectrometry contribute to the enhanced detection of lipids in cancer cell spheroids. Chinese Chemical Letters, 2024, 35(4): 108895-. doi: 10.1016/j.cclet.2023.108895
13. [13]
  Yanjing Li , Jiayin Li , Yuqi Chang , Yunfeng Lin , Lei Sui . Tetrahedral framework nucleic acids promote the proliferation and differentiation potential of diabetic bone marrow mesenchymal stem cell. Chinese Chemical Letters, 2024, 35(9): 109414-. doi: 10.1016/j.cclet.2023.109414
14. [14]
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15. [15]
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16. [16]
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17. [17]
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18. [18]
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19. [19]
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20. [20]
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沈阳化工大学材料科学与工程学院沈阳 110142