Citation: Qian Li, Bodong Kang, Libin Wang, Tao Chen, Yu Zhao, Shilun Feng, Rongjing Li, Hongtian Zhang. Microfluidics embedded with microelectrodes for electrostimulation of neural stem cells proliferation[J]. Chinese Chemical Letters, ;2022, 33(3): 1308-1312. doi: 10.1016/j.cclet.2021.08.006 shu

Microfluidics embedded with microelectrodes for electrostimulation of neural stem cells proliferation

Qian Li ^{a, b} ,
Bodong Kang ^{a, b} ,
Libin Wang ^{a, b} ,
Tao Chen ^{a, b, *,} ,
Yu Zhao ^{a, b} ,
Shilun Feng ^{c, *,} ,
Rongjing Li ^{a, b} ,
Hongtian Zhang ^d

a.
Institute of Advanced Photonics Technology, Faculty of Materials and Manufacturing, Beijing University of Technology, Beijing 100124, China
b.
Key Laboratory of Trans-scale Laser Manufacturing Technology (Beijing University of Technology), Ministry of Education, Beijing 100124, China
c.
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China
d.
Department of Neurosurgery, The 7th Medical Center, General Hospital of the Chinese People's Liberation Army, Beijing 100700, China

chentao@bjut.edu.cn

shilun.feng@mail.sim.ac.cn

Received Date: 26 May 2021
Revised Date: 5 July 2021
Accepted Date: 2 August 2021
Available Online: 8 August 2021
Fund Project: the Key Scientific and Technological Projects of the Beijing Education Commission KZ201910005009

Figures(5)

The regeneration of the injured nerve and recovery of its function have brought attention in the medical field. Electrical stimulation (ES) can enhance the cellular biological behavior and has been widely studied in the treatment of neurological diseases. Microfluidic technology can provide a cell culture platform with the well-controlled environment. Here a novel microfluidic/microelectrode composite microdevice was developed by embedding the microelectrodes to the microfluidic platform, in which microfluidics provided a controlled cell culture platform, and ES promoted the NSCs proliferation. We performed ES on rat neural stem cells (NSCs) to observe the effect on their growth, differentiation, proliferation, and preliminary explored the ES influence on cells in vitro. The results of immunofluorescence showed that ES had no significant effect on the NSCs specific expression, and the NSCs specific expression reached 98.9% ±0.4% after three days of ES. In addition, ES significantly promoted cell growth and the cell proliferation rate reached 49.41%. To conclude, the microfluidic/microelectrode composite microdevice can play a positive role in the nerve injury repair and fundamental research of neurological diseases.
- Microfluidics,
- Microelectrode,
- Electrical stimulation,
- NSCs,
- Proliferation
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