Citation: Ying-Hao Pan, Xiao-Xiao Chen, Lei Dong, Na Shao, Li-Ya Niu, Qing-Zheng Yang. Visualizing nitric oxide-dependent HIF-1 activity under hypoxia with a lipid droplet-targeting fluorescent probe[J]. Chinese Chemical Letters, ;2021, 32(12): 3895-3898. doi: 10.1016/j.cclet.2021.06.024 shu

Visualizing nitric oxide-dependent HIF-1 activity under hypoxia with a lipid droplet-targeting fluorescent probe

Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, China

dl030131018@163.com

niuly@bnu.edu.cn

Received Date: 12 March 2021
Revised Date: 9 June 2021
Accepted Date: 9 June 2021
Available Online: 15 June 2021

Figures(6)

Evaluating the correlation between hypoxia inducible factor 1 (HIF-1) and nitric oxide (NO) generated under hypoxia is of great significance. In this work, we developed a fluorescent probe for the monitor of HIF-1 activity influenced by NO under hypoxia in hepatoma cells with dual-targeting for hepatocyte and lipid droplet (LD). The probe shows excellent selectivity to NO and high sensitivity with 6000-fold fluorescence enhancement. Live cell imaging experiments revealed the probe's capability of imaging exogenous and endogenous NO with specific in LDs of HepG2 cells. For cells under hypoxia, HIF-1 induced LD level is observed to correlate with NO level. This work provides the in-situ visualization of NO-dependent HIF-1 upregulation through LD accumulation.
- Bodipy,
- Fluorescent probe,
- HIF-1,
- Lipid droplet,
- Nitric oxide
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