Citation: Hongfei Jiang, Wujun Chen, Jie Wang, Renshuai Zhang. Selective N-terminal modification of peptides and proteins: Recent progresses and applications[J]. Chinese Chemical Letters, ;2022, 33(1): 80-88. doi: 10.1016/j.cclet.2021.06.011 shu

Selective N-terminal modification of peptides and proteins: Recent progresses and applications

The Affiliated Hospital of Qingdao University and Qingdao Cancer Institute, Qingdao 266071, China

wangj91@qdu.edu.cn

zhangrenshuai@qdu.edu.cn

Received Date: 7 April 2021
Revised Date: 2 June 2021
Accepted Date: 7 June 2021
Available Online: 11 June 2021

Figures(7)

Numerous strategies for linking desired chemical probes with target peptides and proteins have been developed and applied in the field of biological chemistry. Approaches for site-specific modification of native amino acid residues in test tubes and biological contexts represent novel biological tools for understanding the role of peptides and proteins. Selective N-terminal modification strategies have been broadly studied especially in the last 10 years, as N-terminal positions are typically solvent exposed and provide chemically distinct sites for many peptide and protein targets, making N terminus distinct from other functional groups. A growing number of chemical and enzymatic techniques have been developed to modify N-terminal amino acids, and those techniques have the potential in the fields of medicine, basic research and applied materials science. This review focuses on appraising modification methodologies with the potential for biological applications from the past 10 years.
- N-terminal modification,
- N-terminal ligation,
- Terminus modification,
- Peptide modification,
- Protein modification
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