Citation: Yuanyuan Wu, Lei Zhao, Yaran Chang, Liang Zhao, Guangsheng Guo, Xiayan Wang. Ultra-thin temperature controllable microwell array chip for continuous real-time high-resolution imaging of living single cells[J]. Chinese Chemical Letters, ;2021, 32(11): 3446-3449. doi: 10.1016/j.cclet.2021.05.034 shu

Ultra-thin temperature controllable microwell array chip for continuous real-time high-resolution imaging of living single cells

Yuanyuan Wu ^a ,
Lei Zhao ^b ,
Yaran Chang ^a ,
Liang Zhao ^a ,
Guangsheng Guo ^{a, c} ,
Xiayan Wang ^{a, *,}

a.
Center of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology, Beijing 100124, China
b.
Lanzhou University Second Hospital, Lanzhou 730030, China
c.
Minzu University of China, Beijing 100081, China

xiayanwang@bjut.edu.cn

Received Date: 5 March 2021
Revised Date: 31 March 2021
Accepted Date: 17 May 2021
Available Online: 24 May 2021

Figures(4)

Single-cell imaging, a powerful analytical method to study single-cell behavior, such as gene expression and protein profiling, provides an essential basis for modern medical diagnosis. The coding and localization function of microfluidic chips has been developed and applied in living single-cell imaging in recent years. Simultaneously, chip-based living single-cell imaging is also limited by complicated trapping steps, low cell utilization, and difficult high-resolution imaging. To solve these problems, an ultra-thin temperature-controllable microwell array chip (UTCMA chip) was designed to develop a living single-cell workstation in this study for continuous on-chip culture and real-time high-resolution imaging of living single cells. The chip-based on ultra-thin ITO glass is highly matched with an inverted microscope (or confocal microscope) with a high magnification objective (100 × oil lens), and the temperature of the chip can be controlled by combining it with a home-made temperature control device. High-throughput single-cell patterning is realized in one step when the microwell array on the chip uses hydrophilic glass as the substrate and hydrophobic SU-8 photoresist as the wall. The cell utilization rate, single-cell capture rate, and microwell occupancy rate are all close to 100% in the microwell array. This method will be useful in rare single-cell research, extending its application in the biological and medical-related fields, such as early diagnosis of disease, personalized therapy, and research-based on single-cell analysis.
- Ultra-thin microchip,
- Temperature controllable,
- Living single cells,
- One-step patterning,
- Continuous culture,
- High-resolution imaging
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