Citation: Lei Feng, Zhenhao Tian, Ming Zhang, Xin He, Xiangge Tian, Zhenlong Yu, Xiaochi Ma, Chao Wang. Real-time identification of gut microbiota with aminopeptidase N using an activable NIR fluorescent probe[J]. Chinese Chemical Letters, ;2021, 32(10): 3053-3056. doi: 10.1016/j.cclet.2021.03.056 shu

Real-time identification of gut microbiota with aminopeptidase N using an activable NIR fluorescent probe

Lei Feng ^{a, 1} ,
Zhenhao Tian ^{c, 1} ,
Ming Zhang ^a ,
Xin He ^a ,
Xiangge Tian ^a ,
Zhenlong Yu ^a ,
Xiaochi Ma ^{a, b, *,} ,
Chao Wang ^{a, *,}

a.
Dalian Key Laboratory of Metabolic Target Characterization and Traditional Chinese Medicine Intervention, College of Pharmacy, Academy of Integrative Medicine, The National & Local Joint Engineering Research Center for Drug Development of Neurodegenerative Disease, Dalian Medical University, Dalian 116044, China
b.
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China
c.
School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, China

maxc1978@163.com

wach_edu@sina.com

Received Date: 20 January 2021
Revised Date: 19 March 2021
Accepted Date: 22 March 2021
Available Online: 23 March 2021

Figures(5)

A NIR fluorescent probe (DDAA) derived from fluorophore DDAO with alanine as the recognition group was developed for sensing aminopeptidase N (APN) in gut microbiota. Using DDAA as the real-time guidance tool for the fluorescence imaging of intestinal microorganism, target bacteria and saccharomycete possessing active APN were identified successfully from human feces.
- Aminopeptidase N,
- Fluorescent probe,
- Gut microbiota,
- Bacteria,
- Fungi
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