Citation: Xingshang Xu, Nongyue He. Application of adaptive pressure-driven microfluidic chip in thyroid function measurement[J]. Chinese Chemical Letters, ;2021, 32(5): 1747-1750. doi: 10.1016/j.cclet.2021.01.008 shu

Application of adaptive pressure-driven microfluidic chip in thyroid function measurement

Xingshang Xu ^{a, c} ,
Nongyue He ^{a, b, *,}

a.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
b.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China
c.
Nanjing Lanyu Biotechnology Co., Ltd., Nanjing 210000, China

nyhe1958@163.com

Received Date: 3 December 2020
Revised Date: 4 January 2021
Accepted Date: 4 January 2021
Available Online: 9 January 2021

Figures(3)

The improvement in accuracy of in vitro diagnosis has always been the focus of early screening of thyroid dysfunction. We constructed a microfluidic chip based on a polystyrene polymer substrate. Total triiodothyronine (TT3), total thyroxine (TT4), free triiodothyronine (FT3), free thyroxine (FT4), and thyrotropin (TSH) in human whole blood samples were analysed by fluorescence immunoassay to evaluate thyroid function. The results indicate that the microfluidic chip shows a good linear relationship in the detection of TT3, TT4, FT3, FT4, and TSH standards, and the correlation coefficient (r) is not less than 0.9900. In addition, the chip also has strong anti-interference (RSD% ≤ 5%) and good repeatability (CV ≤ 8%), and its inter-batch differences are small (CV ≤ 15%). The results of practical application in clinical thyroid function measurement indicated its high accuracy (r ≥ 0.9900). It provides a new method for the determination of thyroid function and lays a foundation for subsequent clinical application.
- Microfluidic chip,
- Adaptive pressure drive,
- Thyroid dysfunction,
- In vitro diagnosis,
- Whole blood injection
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