Citation: Shan Wang, Beitong Zhu, Bingya Wang, Xinhua Cao, Lei Zhu, Ji-Ting Hou, Lintao Zeng. Revealing HOCl burst from endoplasmic reticulum in cisplatin-treated cells via a ratiometric fluorescent probe[J]. Chinese Chemical Letters, ;2021, 32(5): 1795-1798. doi: 10.1016/j.cclet.2020.12.039 shu

Revealing HOCl burst from endoplasmic reticulum in cisplatin-treated cells via a ratiometric fluorescent probe

Shan Wang ^{a, d, 1} ,
Beitong Zhu ^{b, 1} ,
Bingya Wang ^d ,
Xinhua Cao ^d ,
Lei Zhu ^a ,
Ji-Ting Hou ^{c, d, **,} ,
Lintao Zeng ^{a, b, *,}

a.
Institute of Biomedical Materials Industry Technology, Hubei Engineering University, Xiaogan 432000, China
b.
College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China
c.
Key Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Hainan Trauma and Disaster Rescue, College of Emergency and Trauma, Hainan Medical University, Haikou 571199, China
d.
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China

^**

Received Date: 27 October 2020
Revised Date: 21 December 2020
Accepted Date: 23 December 2020
Available Online: 25 December 2020

Figures(6)

The reactive oxygen species (ROS) are tightly associated with endoplasmic reticulum (ER) stress. Thus, the deep and visual insight of aberrant ROS fluctuations in the ER can help us better investigate the ER stress-associated pathology. In this work, a fluorescent probe ERC for HOCl detection in the ER based on phenothiazine-derived coumarin platform was developed. In the presence of HOCl, ERC exhibited an emission change from 609 nm to 503 nm within seconds. It also showed high sensitivity (0.44 μmol/L) and superb photostability. Significantly, ERC displayed low cytotoxicity, good cell membrane permeability, and appreciable ER-targetability. Ultimately, the probe was successfully utilized to image exogenous and endogenous HOCl in living cells and reveal the HOCl burst in cisplatin-treated cancer cells.
- Fluorescent probes,
- Ratiometric,
- HOCl,
- Endoplasmic reticulum,
- Cisplatin
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沈阳化工大学材料科学与工程学院沈阳 110142